Preparation method of anti-hepatitis B surface antigen-antibody based on green fluorescent protein luminescence structural domain labeling

A technology of green fluorescent protein and light-emitting structure, which is applied in the field of preparation of anti-hepatitis B surface antigen antibody, can solve the problems of large size of green fluorescent protein and the influence of correct folding of the target protein, and achieve the effect of improving accuracy

Inactive Publication Date: 2016-06-29
SHENZHEN SHENGBIZHI TECH DEV CO LTD
View PDF9 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, GFP is too bulky, which will affect the c

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of anti-hepatitis B surface antigen-antibody based on green fluorescent protein luminescence structural domain labeling
  • Preparation method of anti-hepatitis B surface antigen-antibody based on green fluorescent protein luminescence structural domain labeling
  • Preparation method of anti-hepatitis B surface antigen-antibody based on green fluorescent protein luminescence structural domain labeling

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] refer to figure 1 , figure 1 It is a schematic flowchart of the preparation method of the anti-hepatitis B surface antigen antibody based on the green fluorescent protein light-emitting domain label of the present invention. The preparation method of the anti-hepatitis B surface antigen antibody labeled based on the green fluorescent protein light-emitting domain comprises the following steps:

[0037] S1: the first DNA sequence of the synthetic fusion gene GFP-F-Domain-anti-HBsAg;

[0038] Specifically, the first DNA sequence of the fusion gene GFP-F-Domain-anti-HBsAg includes the encoding green fluorescent protein GFP light-emitting domain GFP- The DNA sequence of F-Domain, the DNA sequence encoding anti-HbsAg protein located at the 3' end, and the connecting peptide DNA sequence located between the 5' end and the 3' end. The GFP-F-Domain DNA sequence is shown in the nucleotide sequence table; the GFP-F-Domain protein sequence is shown in the amino acid sequence ta...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of an anti-hepatitis B surface antigen-antibody based on green fluorescent protein luminescence structural domain labeling. The preparation method with a genetic engineering means adopted includes the following steps that a first DNA sequence of a fusion gene GFP-F-Domain-anti-HBsAg is synthesized; amplification is carried out with the first DNA sequence as a template, and a second DNA sequence is obtained; the second DNA sequence is connected to an expression vector pET22b plasmid, and first target clone groups are obtained; the first target clone groups are subjected to inducible expression, and a first bacteria colony is obtained; the first bacteria colony obtained after inducible expression is broken and purified, and the anti-hepatitis B surface antigen-antibody based on green fluorescent protein luminescence structural domain labeling is obtained. By means of the preparation method, the accuracy of quantitative detection of hepatitis B surface antigens is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of an anti-hepatitis B surface antigen antibody marked based on a green fluorescent protein light-emitting domain. Background technique [0002] According to the World Health Organization, 2 billion people worldwide have been infected with HBV. Nearly 1 million people die every year from diseases such as liver cirrhosis or liver cancer caused by chronic hepatitis B virus infection. Quantitative detection of hepatitis B surface antigen (HBsAg) is an important means to evaluate the therapeutic effect of chronic hepatitis B. The 2015 edition of the "Guidelines for the Prevention and Treatment of Chronic Hepatitis B" issued by the Chinese Medical Association pointed out: quantitative monitoring of hepatitis B surface antigen in serum can be used to predict disease progression, antiviral efficacy and prognosis, and the slowest HBsAg disappearance after drug withdrawa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/70C07K19/00C07K1/14
CPCC07K16/082C07K2319/60C12N15/62C07K1/14C07K16/00C12N15/65
Inventor 周晓莹胡立夫英格玛·恩伯格埃姆郎·纳瓦什
Owner SHENZHEN SHENGBIZHI TECH DEV CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap