Small molecular peptide composition of Dendrobium officinale Kimura et Migo as well as extraction method and application of small molecular peptide composition

Abstract

The invention relates to an extraction method of small molecular peptide composition of Dendrobium officinale Kimura et Migo. The extraction method comprises steps as follows: (1), pretreatment of raw materials; (2), denaturation of Dendrobium officinale Kimura et Migo protein; (3), composite enzymatic hydrolysis of the Dendrobium officinale Kimura et Migo protein; (4), filtering and impurity removing; (5), dewatering and drying. The small molecular peptide composition of Dendrobium officinale Kimura et Migo contains small molecular peptides which are obtained through hydrolysis of the Dendrobium officinale Kimura et Migo protein, the molecular weight of the small molecular peptides is mainly distributed in a range from 100 D to 700 D, and the small molecular peptide composition further contains polysaccharide, dendrobine, amino acid, stilbenoid, stilbenoid derivatives, vitamins and trace substances and can be applied to medicine raw materials, healthcare food and cosmetics. The molecular weight of a product is mainly distributed in a range from 100 D to 700 D, most of the obtained small molecular peptides are in micromolecular structures such as dipeptides, tripeptides, tetrapeptides and the like which are quite easily absorbed by a human body, and the small molecule peptides with the molecular weight distributed in 60-700 D accounts for 90% or higher of the total weight of the protein.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for extracting a small molecule peptide composition from Dendrobium officinale, the small molecule peptide composition extracted by the method, and the small molecule peptide composition used in medical raw materials, health care, etc. Applications in food and beauty cosmetics. Background technique [0002] Dendrobium officinale (Latin name: Dendrobium officinale Kimura et Migo), also known as black knot grass, Yunnan iron, iron bucket. It is the dry stem of Dendrobium candidum, which is rich in polysaccharides, dendrobine, amino acids, stilbenes and their derivatives, and various volatile components. Research by He Tieguang and others found that Dendrobium candidum contains 17 kinds of amino acids, and the wild product of Dendrobium candidum Amino acid content reaches 133.1mg / g, among which, the content of essential amino acids such as glutamic acid and asparti...

AI Technical Summary

Problems solved by technology

But the Dendrobium candidum preparations obtained by these methods contain a large amount of unnecessary substances, and even have a special odor. In addition, the purity of its active ingredients is still not enough, especially the protein usually retains almost complete macromolecular forms

The bioavailability of proteins in the form of macromolecules is generally low, which is unfavorable in the health care and cosmetic industries

[0004] Therefore, people need to use various hydrolysis methods to convert proteolysis into peptides that can be directly absorbed in the human body without digestion, so as to improve its bioavailability. The methods of proteolysis mainly include acid method, alkali method and enzymatic method. Among them, the acid method and the alkali method have the disadvantages of high cost, complicated process, difficult control of the degree of hydrolysis, and serious environmental pollution, and in the case of high alkali or high acid, proteins react with impurities such as sugar and oil to produce brown substances. , and even produce harmful substances, which will affect the quality of the final peptide product

The reaction of enzymatic protein hydrolysis is relatively mild, and no adverse side reactions occur, but the singleness of enzyme action makes it difficult to achieve the ideal hydrolysis effect by one enzyme, and the obtained products have disadvantages such as wide molecular weight distribution and low proportion of active peptides

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