Method For Separating And Purifing Functional Ingredients From Placenta By Using Supercritical Fluid Technology

Abstract

The present invention provides a method to separate and purify functional ingredients in placenta by using supercritical fluid technology, wherein, placenta extract liquid and supercritical CO2 solvent are guided into a fractionation tank constantly in a preset velocity at a preset temperature and pressure to extract peptides, proteins and other functional ingredients. And then, the solution is conveyed through the three fractionation tanks at a preset temperature and depressurized gradually to separate, fractionate and purify the content of placenta peptide and protein, so as to obtain the high purity of peptides, proteins, active factors and other functional ingredients. The method provided by the present invention adopts a safe and non-toxic supercritical solvent, does not have the safety doubt, such as the residual solvent, etc., and is environment friendly and safe. The supercritical solvent can be recycled and reutilized, and compared with a known method of extracting and separating the placenta peptides and proteins, the method of the present invention can be in mass production and continuous operation, and has great practical value.

Description

technical field [0001] The present invention is related to the technology of separating active components in placenta, more specifically, it refers to a method for separating and purifying functional components in placenta with supercritical fluid technology. Background technique [0002] The placenta is the combined structure between the fetus and the maternal tissue, providing all the nutrients and protection for the embryo. Scientific research has found that the placenta is rich in growth factors, hormones, proteins, peptides, nucleic acids, antibodies and other concentrated nutrients, which can promote The health of body tissues. It contains rich growth factors such as hepatocyte growth factor, nerve growth factor, epidermal fibrocytokine, insulin growth factor, platelet-derived growth factor, hematopoietic cell growth factor, vascular endothelial cytokine and other growth factors that promote stem cells. These growth factors have Regulates the autonomic nervous system,...

AI Technical Summary

Problems solved by technology

[0004] Existing methods for extracting and purifying placenta-related functional components are complicated, and the separation and purification of functional components such as peptides, proteins, and activating factors in placenta are time-consuming and require the use of a large amount of organic solvents, which may interact with the placenta during the production process. In the case of chemical reactions of the functional components, not only the solvent removal and concentration process after the production is completed, the heating and evaporation of the solvent will easily cause partial damage to the functional components, and the solvent will easily remain in the isolate or purified product, resulting in insufficient purity of the functional components. There are improvements

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