Juniperus sabina total flavonoid extract as well as preparation method and application thereof

Abstract

The invention relates to the technical field of juniperus sabina, and in particular relates to a juniperus sabina total flavonoid extract as well as a preparation method and an application thereof. The juniperus sabina total flavonoid extract is obtained according to the following method: crushing juniperus sabina branches and leaves, performing degreasing after drying, performing reflux extraction after degreasing by using an ethanol solution, performing concentration after extraction, removing impurities by virtue of a silica gel column, and performing enrichment and purification by virtue of macroporous resin to obtain the juniperus sabina total flavonoid extract provided by the invention. The method, in comparison with a traditional method, has the following advantages that (1) the use of acid and lead salts is avoided, the components are stable and cannot be hydrolyzed easily, and no heavy metal is remained; (2) after removing oil-soluble impurities by using silica gel, the pollution of the macroporous resin can be effectively reduced, and the macroporous resin is easy to regenerate; and (3) the enrichment and purification of flavonoid components in juniperus sabina can be realized in a closed environment, the total flavonoid content is high, the process method is simple to operate, is low in cost and is pollution-free, and meanwhile, the juniperus sabina total flavonoid extract provided by the invention has a relatively good effect of resisting inflammation and easing pain, and can be used for treating arthritis diseases.

Description

technical field [0001] The invention relates to the technical field of Xinjiang Junbai, and relates to an extract of Xinjiang Junbai total flavonoids and a preparation method and application thereof. Background technique [0002] Xinjiang Juniperus sabina, also known as Sandy Cypress, Chazi Juniper and Stinky Cypress, is an evergreen creeping shrub of the genus Juniperaceae, widely distributed in the arid barren hills and sandy lands of Xinjiang, Gansu, Inner Mongolia and northern Shaanxi. Because of its ability to resist wind erosion, maintain water and soil, fix sand and protect slopes, and improve the environment, this plant has begun to be cultivated on a large scale in the above-mentioned areas. Xinjiang juniper branches and leaves are commonly used in the treatment of rheumatoid arthritis, rheumatoid arthritis and other diseases in my country, and are recorded in medical literature such as "Uygur Medicine Records", "Xinjiang Chinese Herbal Medicine Handbook" and "Chine...

AI Technical Summary

Problems solved by technology

[0003] The present invention provides an extract of total flavonoids from Sinica cypress and its preparation method and application, which overcomes the deficiencies in the prior art and can effectively solve the problems of low total flavonoid content and easy extraction of total flavonoids in existing methods for extracting total flavonoids from Sinica cypress. Hydrolysis of flavonoid glycosides into aglycones, cumbersome operation and the introduction of heavy metals, fat-soluble components can not be removed cleanly, affecting the quality of total flavonoid extracts, and also affecting the regeneration of macroporous adsorption resins