Standard gene for molecular identification of pterocarpus santalinus and molecular identification method

A Hainan huanghuali, standard gene technology, applied in the field of molecular biology, can solve the problems of unguaranteed identification accuracy, complicated operation process, and sample damage

Inactive Publication Date: 2016-07-20
THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, microscopic anatomy is mostly used to identify Hainan huanghuali. The operation process is complicated and the sample is seriously damaged. Moreover, with the advancement of counterfeiting technology, the identification accuracy of these methods cannot be guaranteed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] In the experiment, 4 Hainan huanghuali samples from different sources were used to extract the sample DNA with the DNA extraction kit from TIANGENE Company. Forward primer used: TTAGCCTTTGTTTGGCAAG, reverse primer: AGAGTTTGAGAGTAAGCAT, PCR amplification conditions were pre-denaturation at 94°C for 4 minutes, followed by denaturation at 94°C for 45 seconds, annealing at 50°C for 45 seconds, and extension at 72°C for 1.5 minutes. A total of 35 cycles were performed. Extend at 72°C for 10 min. The obtained products were compared after sequencing, and the homology among the samples was maintained at more than 99%, and the obtained sequences were compared with other species online, and the homology was below 78%.

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Abstract

Pterocarpus santalinus is also called dalbergia odorifera and is a papilionaceae dalbergia plant. Due to the good quality, small distribution area, long maturing period and small existing resource quantity, the market price keeps rising. Under the motivation of huge benefit, the counterfeits of pterocarpus santalinus spread unchecked, and how to accurately identify pterocarpus santalinus becomes the most challenge in the pterocarpus santalinus trading market. The invention provides a standard gene for molecular identification of pterocarpus santalinus and a molecular identification method; and by adopting the method to identify pterocarpus santalinus, the method has the characteristics of quickness, accuracy, low sample damage and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a standard gene for plant molecular identification and a molecular identification method. Background technique [0002] Hainan Huanghuali ( Dalbergia dorifera T.Chen), also known as Dalbergia Dalbergia, is a plant of the genus Dalbergia in the family Papilionaceae, native to the low-altitude plains and hilly areas of Hainan Island in my country. Because of its solid wood, beautiful patterns and fragrance, huanghuali wood, rosewood, chicken wing wood, and iron wood are also known as the four famous woods in ancient China, and it is one of the two mahogany tree species natively produced in my country. Because the material is hard, beautiful and has a fragrance, it has always been regarded as a high-grade wood. Because of the narrow distribution area and excessive felling, the existing number is very small, and it is listed as a national second-class protected plant. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/166
Inventor 王兵益杨时宇廖礼彬李体初
Owner THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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