Stem cell culture supernate gel and preparation method thereof

A stem cell culture and stem cell technology, applied in medical science, bandages, etc., can solve the problems of high risk, poor stability of stem cell culture supernatant, difficult purification of stem cell culture supernatant, etc., and achieve the effect of leaving no scar and remarkable effect

Active Publication Date: 2016-07-27
江苏迈健生物科技发展股份有限公司
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Problems solved by technology

[0005] Technical problem to be solved: In order to overcome the problems of high risk of traditional fetal bovine serum-containing cell culture system, difficulty in purification of stem cell culture supernatant, and poor stability of stem cell culture supernatant, the present invention provides a stem cell serum-free culture supernatant gel and Its preparation method, the gel is used for skin wound repair, the effect is remarkable and no scar

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  • Stem cell culture supernate gel and preparation method thereof
  • Stem cell culture supernate gel and preparation method thereof
  • Stem cell culture supernate gel and preparation method thereof

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Embodiment 1

[0024] Preparation of mesenchymal stem cells: take fresh and healthy newborn umbilical cord, rinse the umbilical cord with PBS, remove the blood vessels, peel off the Wahrenheit tissue inside, and cut the obtained tissue to 1mm 3 size, added serum-free medium, at 37°C, 5% CO 2 Cultured in an incubator, after the cells were confluent, they were digested and passaged with 0.25% trypsin. The third passage of umbilical cord-derived mesenchymal stem cells was used for preparation of gel.

[0025] The main formula of serum-free medium: human serum albumin: 4g / L-10g / L, glycine: 30.00mg / L, non-essential amino acid: 1mg / L-50mg / L, recombinant human insulin: 1mg / L-10mg / L , human transferrin: 5mg / L-20mg / L.

[0026] The preparation method of stem cell serum-free culture supernatant gel comprises the following steps:

[0027] Step 1: Take the third generation of umbilical cord-derived mesenchymal stem cells and culture them in serum-free medium. When the culture flask is 70% full, cultur...

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Abstract

The invention provides a stem cell culture supernate gel and a preparation method thereof.The gel is prepared from stem cell culture supernate and methylcellulose, and water servers as a solvent.The stem cell culture supernate is obtained by culturing mesenchymal stem cells with a stem cell culture medium containing no serum and then performing collecting and purifying.The preparation method of the gel includes the steps that firstly, the mesenchymal stem cells are cultured with the stem cell culture medium containing no serum, supernate is collected, and supernate I is obtained; the supernate I is centrifuged, ammonium sulfate is added into obtained supernate II, stirring, standing and centrifuging are performed, precipitate is taken, water is added for dissolving, and filtering is carried out; then, obtained protein filter liquor is purified with a gel column, and purified supernate is freeze-dried; finally, freeze-dried supernate is dissolved with sterile water, an obtained water solution is mixed with a methylcellulose water solution in an isopyknic mode, and the stem cell culture supernate gel is obtained.The stem cell culture supernate gel is used for repairing skin wounds and is significant in effect, and no cicatrix remains.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a stem cell culture supernatant gel and a preparation method thereof. Background technique [0002] The wound healing process is a complex process, which involves re-epithelialization of skin defects, hyperplasia of granulation tissue, inflammation, angiogenesis, and wound remodeling. Any problem in any of these processes will lead to wound healing. obstacle. At present, the main method of repairing wounds is surgery, which is to convert open wounds into closed wounds through surgical suturing, so as to accelerate wound healing. However, there are complications such as postoperative scars, excessive wound tension, cracking, or dysfunction after healing. In clinical work, we often encounter some relatively complicated wounds, such as radiation wounds, diabetic feet, wounds after long-term use of hormones, etc. These wounds often have local blood circulati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/0023A61L26/0066A61L26/008A61L2300/252A61L2300/412C08L1/02
Inventor 常菁虞强王丹冷晓燕季夏芸
Owner 江苏迈健生物科技发展股份有限公司
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