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Achromobacter CZ207 strain capable of resisting heavy metal and promoting plant growth and application thereof

A CZ207, a plant growth-promoting technology, applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc., can solve the problems of less restoration, slow growth speed, single absorption of heavy metals, etc.

Inactive Publication Date: 2016-07-27
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with traditional heavy metal remediation such as physical chemistry, phytoremediation has the advantages of simple operation, good treatment effect, no secondary pollution, low remediation cost, and large-scale promotion and application. It has good social and ecological comprehensive benefits, but phytoremediation The method also has bottleneck problems such as slow growth rate, small amount of restoration, easy to be limited by natural and artificial factors, and relatively simple absorption of heavy metals.

Method used

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  • Achromobacter CZ207 strain capable of resisting heavy metal and promoting plant growth and application thereof
  • Achromobacter CZ207 strain capable of resisting heavy metal and promoting plant growth and application thereof

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Embodiment Construction

[0009] The present invention will be described in detail below in conjunction with specific embodiments, but the protection scope of the present invention is not limited to the following embodiments.

[0010] Isolation and identification of strains

[0011] In this example, a plant rhizosphere microorganism was isolated from the rhizosphere soil of a plant grown in an iron mine plant in Enshi Autonomous Prefecture, and named it CZ207. The morphological, physiological and biochemical characteristics of the strain are: Observed on LB solid medium The colony is light yellow, the colony is neat, moist, and the center is convex; microscopic examination of the strain is Gram stain negative, rod-shaped, without spores.

[0012] The CZ207 strain was inoculated into 5ml LB culture medium (peptone 10.0g, yeast extract 5.0g, NaCl10.0g, H 2 O1000mL), 37°C, 200rpm shaker culture for 24 hours, centrifugation at 10000rpm for 1min, collect the bacterial cells in a 1.5ml centrifuge tube, and extract ...

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Abstract

The invention provides an achromobacter sp CZ207 strain capable of resisting heavy metal such as cadmium, copper, lead, nickel and manganese and promoting the plant growth. The achromobacter sp CZ207 strain is preserved in CCTCC (China Center for Type Culture Collection) on March 7, 2016, the address is Wuhan University, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M2016091; a 16S rDNA (Ribosomal Deoxyribose Nucleic Acid) sequence of the achromobacter sp CZ207 strain is as shown in an SEQ ID NO.1. Through observation on an LB solid culture medium, bacterial colonies are light yellow, tidy and moist, and the center parts of the bacterial colonies bulge; through microscopy observation, the bacterial colonies are in gram-negative, are rod-shaped and have no spores. The achromobacter sp CZ207 strain provided by the invention can tolerate multiple heavy metals, can secrete nutrient elements such as plant growth hormone, dissolved inorganic phosphorus and solid nitrogen and has the function of promoting the plant growth.

Description

Technical field [0001] The invention relates to a new strain of Achromobacter, which can tolerate multiple heavy metals such as chromium and cadmium, and particularly relates to the plant growth-promoting effect of the strain and the bioremediation of heavy metal polluted environments. Background technique [0002] With the development of the economy, the demand for heavy metals in all walks of life is increasing. Wastes from industrial metallurgy and electroplating, municipal sewage containing heavy metals, agricultural fertilizers, and pesticides have all become the main channels for heavy metals to enter the environment. Heavy metal pollution not only reduces soil fertility, inhibits the growth and reproduction of soil microorganisms, and affects the yield and quality of crops, but also affects animal and human health through the food chain. Traditional heavy metal remediation mainly uses physical and chemical methods, such as precipitation, ion exchange, adsorption, chemical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00B09C1/10C12R1/025
CPCA01N63/10B09C1/10C12N1/205C12R2001/025
Inventor 程国军田梦洋曾小波杜冬云何冬兰李晓华殷杰彭杨
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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