Achromobacter CZ207 strain capable of resisting heavy metal and promoting plant growth and application thereof
A CZ207, a plant growth-promoting technology, applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc., can solve the problems of less restoration, slow growth speed, single absorption of heavy metals, etc.
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[0009] The present invention will be described in detail below in conjunction with specific embodiments, but the protection scope of the present invention is not limited to the following embodiments.
[0010] Isolation and identification of strains
[0011] In this example, a plant rhizosphere microorganism was isolated from the rhizosphere soil of a plant grown in an iron mine plant in Enshi Autonomous Prefecture, and named it CZ207. The morphological, physiological and biochemical characteristics of the strain are: Observed on LB solid medium The colony is light yellow, the colony is neat, moist, and the center is convex; microscopic examination of the strain is Gram stain negative, rod-shaped, without spores.
[0012] The CZ207 strain was inoculated into 5ml LB culture medium (peptone 10.0g, yeast extract 5.0g, NaCl10.0g, H 2 O1000mL), 37°C, 200rpm shaker culture for 24 hours, centrifugation at 10000rpm for 1min, collect the bacterial cells in a 1.5ml centrifuge tube, and extract ...
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