Sugarcane polyphenols extract and preparation method and application thereof
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An extract and technology of sugarcane, applied in the field of sugarcane polyphenol extract and its preparation, can solve problems such as myocardial infarction and cancer induction, and achieve the effects of low cost, strong hypoglycemic activity, and simple production process
Active Publication Date: 2016-08-03
SOUTH CHINA UNIV OF TECH
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The current hypoglycemic drugs play an important role in the treatment of type 2 diabetes, but long-term use of these drugs will pose potential threats to human health, such as inducing cancer, myocardial infarction, and fractures
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Embodiment 1
[0034] The preparation method of a kind of sugarcane polyphenol extract of the present embodiment, its extraction technology roadmap is as follows figure 1 As shown, the specific extraction steps are as follows:
[0035] (1) Purchase fresh raw sugar cane, crush the sugar cane after peeling, and dry at 40°C. After passing through a 40-mesh sieve, the coarse powder was obtained and stored at -20°C. Take 300g of the above-mentioned sugarcane coarse powder, use 3000mL of 60% ethanol aqueous solution with a volume ratio of 60%, extract for 90min, filter, extract 3 times, and vacuum filter, combine the filtrates, and concentrate under reduced pressure to extract A 1 ;
[0036] (2) Extraction A obtained from step (1) 1 Add 100 mL of water to disperse, extract 3 times with petroleum ether, add 100 mL of petroleum ether each time, collect the petroleum ether extract and water phase, extract the water phase with 100 mL of ethyl acetate for 3 times, collect the ethyl acetate phase, an...
Embodiment 2
[0039] The preparation method of a kind of sugarcane polyphenol extract of the present embodiment, its extraction technology roadmap is as followsfigure 1 As shown, the specific extraction steps are as follows:
[0040] (1) Take 300g of dried bagasse, crush it and pass it through a 40-mesh sieve to obtain a coarse powder, use 1500mL of 95% ethanol aqueous solution at 50°C for 90min with ultrasonic assistance, and extract 3 times at 350w ultrasonic power. After vacuum filtration, the combined filtrates were concentrated under reduced pressure to extract B 1 ;
[0041] (2) Extraction B obtained from step (1) 1 Add 100 mL of water to disperse, extract 3 times with petroleum ether, add 100 mL of petroleum ether each time, collect the petroleum ether extract and water phase, extract the water phase with 100 mL of ethyl acetate for 3 times, collect the ethyl acetate phase, and concentrate under reduced pressure to the extract to obtain the extract B 2 ;
[0042] (3) The concentr...
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Abstract
The invention belongs to the technical field of health care food and discloses sugarcane polyphenols extract and a preparation method and application thereof.The preparation method includes the steps that sugarcane or bagasse is taken, crushed and then sieved, coarse powder is obtained, then the coarse powder is extracted with an ethanol solution, filtering is conducted to remove filter residues, and filtrate is subjected to decompression and concentrated to extractum; water is added to the obtained extractum for dispersion, petroleum ether is adopted for extraction, a petroleum ether phase and a water phase are obtained, the water phase is extracted with ethyl acetate, an ethyl acetate phase is collected, and the ethyl acetate phase is subjected to decompression and concentrated to extractum; then chromatographic separation is conducted with a polyamide column, ethyl alcohol is subjected to gradient elute, eluate with the alcohol volume concentration being 30% is collected, the eluate is concentrated and dried, and the sugarcane polyphenols extract is obtained.The sugarcane polyphenols extract is made of staple crops, namely sugarcane, multistage extraction and column chromatography gradient elution separation and purification are conducted, and a product has higher hypoglycemic activity and can be used for food additives and functional food.
Description
technical field [0001] The invention belongs to the technical field of health food, and in particular relates to a sugarcane polyphenol extract and its preparation method and application. Background technique [0002] Diabetes is a multifactorial endocrine disorder syndrome, including carbohydrate, protein and fat metabolism disorders. Diabetes patients are distributed in various countries all over the world, and the number of patients is also increasing year by year. Diabetes and its complications not only seriously affect the quality of life of patients, but also bring a heavy economic burden to individuals and society. Type 2 diabetes accounts for about 90-95% of all diagnosed cases of diabetes. The occurrence of type 2 diabetes is largely due to unreasonable living habits and dietary patterns, and excessive intake of sugar, which leads to the loss of function of insulin and its related receptors. Current hypoglycemic drugs play an important role in the treatment of ty...
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