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A method for preparing glyceride rich in epa and dha by enzymatic catalysis

A technology for the catalytic preparation of glycerides, which is applied in the deep processing and modification of oils and fats. It can solve the problems of unmentioned recycling, unfavorable production safety, waste of by-products, etc., to increase the content of triglycerides and the content of EPA and DHA, The effect of saving investment and high reaction efficiency

Active Publication Date: 2019-07-19
广东至润油脂食品工业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the hydrolysis method, the cost of using ethanol is obviously higher than that of water, and ethanol is a flammable and volatile solvent, which is not good for production safety
In addition, the by-products of fatty acid ethyl esters obtained from alcoholysis of fish oil still contain a certain amount of EPA and DHA in the form of ethyl esters, but none of these documents mention their recycling, resulting in waste of by-products

Method used

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  • A method for preparing glyceride rich in epa and dha by enzymatic catalysis
  • A method for preparing glyceride rich in epa and dha by enzymatic catalysis
  • A method for preparing glyceride rich in epa and dha by enzymatic catalysis

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A kind of method that utilizes enzyme catalysis to prepare rich in EPA and DHA glyceride, technological process is as follows figure 2 shown, including the following steps:

[0046] (1) Add 30.0g of refined fish oil from Noah Sano Biological Co., Ltd., 30.0mL of potassium phosphate buffer solution (0.1mol / L, pH=6) and 6.0mg of Lipase AY "Amano" 400SD from Amano Enzyme Co., Ltd. To a 100mL Erlenmeyer flask, fill the flask with nitrogen to exclude air, and seal it. Put the Erlenmeyer flask into a shaker, set the shaking rate to 200r / min, shaker temperature to 35°C, and maintain the reaction for 10h. After the reaction was completed, the resulting mixture was centrifuged to separate liquids. The resulting upper oil phase is a mixture of glycerides rich in EPA and DHA and free fatty acids, and the lower layer is an aqueous phase containing lipase.

[0047] (2) A total of 200 g of the upper layer oil phase obtained in the aforementioned step (1) was accumulated, which wa...

Embodiment 2

[0055]This implementation example is the same as embodiment 1 except the following technical features:

[0056] The buffer solution used in step (1) is 0.1mol / L potassium phosphate solution (pH 7), the buffer solution volume used is 45mL; the amount of enzyme added is 15mg; the reaction temperature is 50°C, and the reaction time is 8h.

[0057] The temperature of the second stage molecular distillation used in step (2) is 140°C. After molecular distillation, the gained heavy phase quality is 106.6g, and corresponding productive rate is 53.3%; Contain 69.0% triglyceride, 30.0% diglyceride, 0.80% monoglyceride, 0.21% free fatty acid in the heavy phase; Among fatty acids, EPA accounts for 25.0%, and DHA accounts for 20.2%. The light phase obtained by molecular distillation is mainly free fatty acids mainly saturated fatty acids, with a mass of 81.2 g and a corresponding yield of 40.6%, in which EPA content is 12.4%, and DHA content is 1.95%.

[0058] The quality of 95% ethanol ...

Embodiment 3

[0062] This implementation example is the same as embodiment 1 except the following technical features:

[0063] The buffer solution used in step (1) is 0.1 mol / L potassium phosphate solution (pH 6.5), the volume of the buffer solution used is 90 mL; the amount of enzyme added is 3.0 mg; the reaction temperature is 25° C., and the reaction time is 24 h.

[0064] The temperature of the second stage molecular distillation used in step (2) is 160°C. After molecular distillation, the gained heavy phase quality is 100.1g, and corresponding productive rate is 50.1%; Contain 64.5% triglyceride, 34.0% diglyceride, 1.20% monoglyceride, 0.24% free fatty acid in the heavy phase; Among the fatty acids, EPA accounts for 23.2%, and DHA accounts for 20.1%. The light phase obtained by molecular distillation is mainly free fatty acids mainly saturated fatty acids, with a mass of 85.2 g, wherein the EPA content is 13.3%, and the DHA content is 2.53%.

[0065] The mass of 95% ethanol (95% etha...

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Abstract

The invention belongs to the technical field of deep processing and modification of oil, and particularly discloses a method for preparing glyceride rich in EPA and DHA through enzyme catalysis .Fish oil rich in EPA and DHA is used as the raw material, efficient selective catalysis of fish oil hydrolysis is conducted with lipase, and glyceride rich in EPA and DHA is obtained through molecular distillation separation purification by strictly controlling hydrolysis conditions including enzyme adding amount, pH, water-oil ratio, temperature and time .To increase the utilization rate of raw materials, EPA and DHA in a free fatty acid product obtained through molecular distillation are recovered with the urea coating method, so that transesterification can be conducted between EPA and DHA subjected to ethyl esterification and glyceride obtained through molecular distillation and rich in EPA and DHA, and the content of triacylglycerol, the content of EPA and the content of DHA in glyceride are further increased .The content of EPA and the content of DHA in triacylglycerol type fish oil obtained with the method can be increased by 25% and 15% or more respectively.

Description

technical field [0001] The invention belongs to the technical field of oil deep processing and modification, and in particular relates to a method for preparing glyceride rich in EPA and DHA by enzymatic catalysis. Background technique [0002] Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are polyunsaturated fatty acids, which are characteristic fatty acids of marine animals and plants. As essential fatty acids that the human body cannot synthesize by itself, they can prevent and treat various diseases such as atherosclerosis, coronary heart disease, cancer, asthma, fatty liver, Alzheimer's disease, and promote the normalization of the central nervous system and sensory organs of infants. Health functions such as development. The content of EPA and DHA in the fish oil glycerides currently on the market is generally on the low side, can only reach about 18% and 12% respectively, can not satisfy people's health care and medicinal needs. Therefore, increasing th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64
CPCC12P7/6454C12P7/6472
Inventor 汪勇刘芳滕英来
Owner 广东至润油脂食品工业有限公司
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