Method for inducing differentiation of induced pluripotent stem cells and method for selecting induced pluripotent stem cells

A technology for pluripotent stem cells and differentiation induction, applied in the field of screening kits for induced pluripotent stem cells

Inactive Publication Date: 2016-08-10
KANEKA CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The stem cells used in Patent Document 1 are embryonic stem cells (ES cells), and there is no description about induced pluripotent stem cells (iPS cells)

Method used

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  • Method for inducing differentiation of induced pluripotent stem cells and method for selecting induced pluripotent stem cells
  • Method for inducing differentiation of induced pluripotent stem cells and method for selecting induced pluripotent stem cells
  • Method for inducing differentiation of induced pluripotent stem cells and method for selecting induced pluripotent stem cells

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Experimental program
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Embodiment

[0070] (1) Preparation of frozen sections

[0071] Normal livers, brains and spinal cords were removed from male, 6-week-old ICR mice. In addition, 1ml / kg of carbon tetrachloride was mixed into olive oil at a ratio of 1:4 to obtain a mixture, and the mixture was administered intraperitoneally to artificially induce drug-induced hepatitis in ICR mice. On the 1st, 2nd, 3rd, and 5th days afterward, hepatitis livers were removed from the ICR mice.

[0072] Embed each organ in Tissue-Tek as an OCT embedding medium R (Sakura Finethechnical) and mounted, and then immersed in liquid nitrogen to make blocks for frozen sections. Cryosections each having a thickness of 6 μm were prepared from these blocks and placed on a circular culture cover glass (poly-L-lysine-coated type, Matsunami Glass). After drying, wash with phosphate buffered saline (Phosphate Buffered Saline: hereinafter abbreviated as PBS, pH7.4) twice, wash the OCT embedding agent and use it for culturing iPS cells.

[...

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Abstract

The present invention addresses the problem of providing a method for highly efficiently inducing differentiation of induced pluripotent stem cells (iPS cells) into cells having a desired function, and a method for selecting, from among the iPS cells prepared above, iPS cells that can be highly efficiently induced to undergo differentiation into the target cells. The method for inducing differentiation of iPS cells, said method comprising culturing iPS cells on a structure that contains a cell and / or a component derived from a cell, wherein the cell in the structure is of the same cell type as the cells having been induced to undergo differentiation.

Description

technical field [0001] The invention relates to a method for inducing differentiation of induced pluripotent stem cells, a screening method for induced pluripotent stem cells, and a screening kit for induced pluripotent stem cells. Background technique [0002] Stem cells play an important role in regenerative medicine. Embryonic stem cells (ES cells), embryonic carcinomatous cells (EC cells), embryonic germ cells (EG cells), nuclear transfer ES cells, and ES cells derived from somatic cells (ntES cells) are known as stem cells capable of differentiating totipotently. and induced pluripotent stem cells (iPS cells); as stem cells having differentiation and pluripotency, somatic stem cells, tissue stem cells, and adult stem cells are known. Among the above, induced pluripotent stem cells (iPS cells) are pluripotent and artificially prepared from somatic cells, so there is no ethical problem caused by destroying embryos and egg cells, and there is no problem of suitability for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12M1/26C12Q1/68
CPCC12N5/0696C12Q1/6881C12Q2600/158C12N2502/00C12N2502/08C12N2502/14C12N2506/45C12N5/0618C12N5/0652C12N5/067
Inventor 里村一人
Owner KANEKA CORP
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