33 results about "Cell Differentiation Induction" patented technology

The novel benzamide derivative represented by formula (1) and the novel anilide derivative represented by formula (13) of this invention has differentiation-inducing effect, and are, therefore, useful a therapeutic or improving agent for malignant tumors, autoimmune diseases, dermatologic diseases and parasitism. In particular, they are highly effective as an anticancer drug, specifically to a hematologic malignancy and a solid carcinoma.

The present invention provides a compound represented by the formula:wherein R1 is an amino group which may be substituted; R2 is a hydrogen atom or a lower alkyl group which may be substituted; X is a methyne group which may be substituted or N(O)m (m is 0 or 1); a ring A is a homo- or hetero-cycle which is substituted by a halogen atom, lower alkyl, lower alkoxy or lower alkylenedioxy; and a ring B is a homo- or hetero-cycle which may be substituted; or a salt thereof, which exhibits excellent cell differentiation-inducing action and cell differentiation-inducing factor action-enhancing action, and is useful in the treatment and prevention of various nerve diseases or bone / joint diseases.

The object of the present invention is to provide a method of preparing excellent cultured muscle cells having high metabolic capacity and insulin responsiveness, and further provide a method for the measurement of sensitive metabolic capacity using the cells. Moreover, its purpose is to provide a culture system / culture apparatus that can smoothly translocate such highly advanced cultured muscle cells intact to activity evaluation systems of a number of drugs. Moreover, the object of the present invention is to provide cultured muscle cells that are very suitable for measurement of the membrane-translocation activity of GLUT4 in an extraneous stimulus-dependent manner such as insulin, etc., and to provide a method for the measurement of the membrane-translocation activity of GLUT4 using the cells. The present invention is a method of preparing myotube cells, comprising a step (1) of culturing myoblast cells, a step (2) of differentiation-inducing the myotube cells into the myoblast cells in a culture medium with a high content of amino acids, and a step (3) of applying an electric pulse to the differentiation-induced myotube cells, and a method for the measurement of insulin-dependent sugar uptake using the myotube cells prepared by said method, and relates to the method for the measurement, comprising applying insulin stimulation by culturing the cells in a culture medium containing insulin, culturing the cells in the culture medium further supplemented with sugar, and measuring the sugar uptake. Furthermore, the present invention relates to a differentiation-type culture myotube cell constitutively expressing a recombinant GLUT4 having a labeled substance at its extra-cellular site, which is prepared by co-culturing wild-type myoblast cells and recombinant myoblast cells constitutively expressing said recombinant GLUT4, and a method for the measurement of membrane-translocation activity of the recombinant GLUT4 using the cells, and particularly a method for the measurement of insulin-dependent sugar uptake activity.

By a method of inducing differentiation of cells having: a step of co-culturing amniotic epithelial cells and amniotic interstitial cells so as to induce their differentiation into predetermined tissue cells, it is possible to efficiently induce differentiation of the amniotic epithelial cells and the amniotic interstitial cells. It is preferable that the amniotic epithelial cells or the amniotic interstitial cells are prepared by being cultured by a predetermined culture method.

A compound represented by the formula: wherein X represents a sulfur atom or an oxygen atom; Y represents an optionally oxidized sulfur atom or an oxygen atom; Z represents a bond or a divalent hydrocarbon group; R1 represents an optionally substituted hydrocarbon group; R2 represents an optionally amidated or esterified carboxyl group; ring A represents an optionally substituted aromatic 5-membered heterocyclic ring; or a salt thereof. A compound of the above formula possesses cell differentiation inducing factor action-enhancing activity and anti-matrix metalloprotease activity and that is useful in the prevention and treatment of bone diseases such as osteoporosis, bone fractures, osteoarthritis and rheumatoid arthritis, arteriosclerosis, cancer metastasis, and diseases based on nerve degeneration.

The invention relates to a valeriana jatamansi jones breeding method, which is characterized in that the field culture transplanting is carried out after tissue culture breeding or seed breeding; the tissue culture breeding seedling comprises measures including sterile system establishing, culture medium adoption as callus culture, propagation culture and rooting culture, exercising seedling and acclimation seedling treatment, and seedling bed and young seedling disease prevention after the planting; and the seed breeding seedling comprises measures including seed collection time, dormancy stage breaking, bud cell differentiation induction and seedling period disease and pest prevention. The method provided by the invention mainly solves the problem of valeriana jatamansi jones breeding industrialization, the reliable guarantee is provided for the planting, the endangered species are effectively protected, and particularly, the obvious progress is realized in the field of endangered medicinal material rescue.

A therapeutic agent and prophylactic agent for a disease accompanying an abnormality in an amount of insulin or insulin response, an agent for an insulin-mimetic action, a food, beverage and feed, an agent for enhancing glucose uptake into a cell, and an agent for inducing differentiation into an adipocyte, characterized in that each comprises as an effective ingredient at least one compound selected from the group consisting of a chalcone compound, an acetophenone compound, a coumarin compound, a phthalide compound, derivatives thereof, and pharmacologically acceptable salts thereof.

A compound represented by the formula:wherein X represents a sulfur atom or an oxygen atom; Y represents an optionally oxidized sulfur atom or an oxygen atom; Z represents a bond or a divalent hydrocarbon group; R1 represents an optionally substituted hydrocarbon group; R2 represents an optionally amidated or esterified carboxyl group; ring A represents an optionally substituted aromatic 5-membered heterocyclic ring; or a salt thereof.A compound of the above formula possesses cell differentiation inducing factor action-enhancing activity and anti-matrix metalloprotease activity and that is useful in the prevention and treatment of bone diseases such as osteoporosis, bone fractures, osteoarthritis and rheumatoid arthritis, arteriosclerosis, cancer metastasis, and diseases based on nerve degeneration.

The invention discloses a method for promoting brown adipose tissue differentiation through SFRP4 and an application. The method comprises treating precursor adipose cells for differentiation by usingSFRP4 active proteins (with different concentrations of 0, 1, 10, 100ng / mL), when the cells grow to 80%, culturing with a cell differentiation induction culture medium I until the cells are fused, adding an induction culture medium II into the cells, inducing for 2-8 days, and replacing the culture medium in the culture bottle with an induction culture medium III, inducing for 2-8 days, observingunder a microscope, after dyeing with oil red O, observing under the microscope again, collecting cells with different differentiation days, extracting total RNA of the cells, detecting expression levels of marker genes UCP-1 and SFRP4 of brown adipose cells by using a real-time quantitative PCR analysis technology after the purity and integrity of the RNA are detected to be qualified, and finally, carrying out statistical analysis. The experimental process is uniform in sampling, and the result reliability is high.

The present invention addresses the problem of providing a method for highly efficiently inducing differentiation of induced pluripotent stem cells (iPS cells) into cells having a desired function, and a method for selecting, from among the iPS cells prepared above, iPS cells that can be highly efficiently induced to undergo differentiation into the target cells. The method for inducing differentiation of iPS cells, said method comprising culturing iPS cells on a structure that contains a cell and / or a component derived from a cell, wherein the cell in the structure is of the same cell type as the cells having been induced to undergo differentiation.

The invention provides an experimental device for high-throughput electrical-stimulation cell differentiation induction and drug controlled release. The device comprises an electrical stimulation signal generator and a cell culture apparatus, wherein the electrical stimulation signal generator comprises a single-chip microcomputer minimum system, a digital-analog converter and a signal regulating module; the output end of the signal regulating module is connected with the running end of a single-pole double-throw switch; an upper cover plate is arranged at the top of the cell culture apparatus; one end, connected with an electric wire, of the upper cover plate is connected with a titanium alloy electrode which is suspended in the cell culture apparatus, while the other end of the upper cover plate is connected with a first static end of a switch; the bottom plate of the cell culture apparatus is a drug release plate which is simultaneously connected with the second static end of the switch and the ground end of the signal regulating module; and a drug-loading conductive high-molecular film is arranged on the drug release plate. The experimental device can be used for generating electrical stimulation signals with random frequencies and amplitudes, and can be added with drugs to carry out high-throughput electrical stimulation signal and drug screening in the process of peripheral stem cell differentiation, so that a wide and reliable experiment basis is provided to stimulation therapy.

A novel method of inducing or producing hepatocytes from human induced pluripotent stem cells at an unprecedented efficiency and functionality. The core of the invention is the use of experimentally discovered mRNAs at multiple critical differentiation decision points along a pluripotent to mesendoderm to endoderm to hepatocytes pathway in a previously unknown manner.

Th invention discloses a stem cell based mammal artificial embryo constructing and animal cloning breeding method and a model thereof. With a mammal ovum zona pellucida or an artificially synthesized structure equivalent to the zona pellucida as a stem cell receptor of an artificial embryo, the stem cell receptor is implanted into stem cells, and differentiation of the stem cells is induced under an in-vitro condition, so that a mammal preimplantation embryo structure is obtained. Two key technical designs include differentiation capacity application of stem cell pluripotency to a primary embryo and early embryo differentiation inducing of a constructed stem cell-ovum zona pellucida complex. Four major technical steps include preparation of the stem cells, preparation of animal ovum or early embryo zona pellucida, artificial embryo construction and cell differentiation inducing, and animal cloning breeding. The method is expected to become an important technical means in the industrialization application animal cloning breeding technology in the future; and meanwhile, the method has an important medical application value.

[Problem]To provide a screening method for a substance having an anti-obesity action and an anti-obesity drug.SolutionA screening method including: a step for contacting a test substance and a synoviolin-gene-expressing cell; and a step for verifying the effect of the test substance on the synoviolin gene expression, or the effect thereof on synoviolin protein activity. An action which reduces the amount of adipose tissue and an action which inhibits induction of adipocyte differentiation are examples of an anti-obesity action. An anti-obesity drug containing, as an active ingredient thereof, an siRNA of synoviolin, a decoy nucleic acid of synoviolin, or an antisense nucleic acid of synoviolin.

Bone marrow erythroid progenitor cell(s) differentiation may be induced and anemia may be treated by administering arginine. Arginine is an excellent differentiation inducer, because it has high safety, can be orally administered, and is widely used.

The present invention relates to a therapeutic agent or prophylactic agent for a disease accompanying an abnormality in an amount of insulin or insulin response, an insulin-mimetic action agent, a food, beverage, or feed, an agent for enhancement of glucose uptake into a cell, and an agent for inducing differentiation into an adipocyte, each comprising as an effective ingredient at least one compound selected from the group consisting of specified compounds having an insulin-mimetic action, derivatives thereof, and pharmacologically acceptable salts thereof.

This invention provides a substituted isoxazolylthiophene compound represented by the formulawherein R1 and R2 individually represent an alkyl group of 1-5 carbon atoms, R3 represents a cyano group or a group CONR5R6 (in which R5 and R6 individually represent a hydrogen atom or an alkyl group of 1-10 carbon atoms), R4 represents an alkyl group of 1-5 carbon atoms or a phenyl group, and n is an integer of 0-2, or a salt thereof.The compounds of the invention are useful for the treatment or prevention of various bone diseases or nerve diseases, because they specifically enhance the action of the cell differentiation induction factors found in a living body.

The invention relates to a scaffold for steering cells into a predetermined direction of cell functionality, preferably a cell differentiation scaffold for steering cells into a predetermined direction of cell differentiation. The scaffold comprises a polydimethylsiloxane (PDMS)-, or rubber-, or silicone-based polymeric surface, and one or more cell functionality-inducing stimuli, preferably one or more cell differentiation-inducing stimuli, coupled to the polymeric surface.

A novel method of inducing or producing pancreatic beta cells from human induced pluripotent stem cells at an unprecedented efficiency and functionality. The core of the invention is the use of experimentally discovered mRNAs at multiple critical differentiation decision points along a pluripotent to mesendoderm to endoderm to pancreatic endocrine cells to pancreatic beta cells pathway in a previously unknown manner.

The invention discloses a multifunctional intimal stem cell hematopoietic molecular material and a method, relates to the field of biological pharmacy, and provides the following scheme aiming at the problems of low cell differentiation induction efficiency, poor cell functionality and the like in the prior art: the multifunctional intimal stem cell hematopoietic molecular material comprises a pluripotent hematopoietic stem cell, a specific gene, a somatic cell, a niche, a lentiviral vector and four transcription factors of Oct4, Sox2, Nanog and LIN28. According to the method provided by the invention, by independently adding the raw materials and the additives into the separated culture medium, the differentiation after the combination of the pluripotent hematopoietic stem cells and the somatic cells under the conditions of constant temperature and normal oxygen concentration can be simulated and realized; and homing, positioning and self-replication of the pluripotent hematopoietic stem cells are induced by utilizing niche obtained on the surfaces of endoosseous membranes and bone trabecula in a marrow cavity of a mammal or from a commercial mammal blood sinus to form and proliferate the APSC pluripoietic stem cells so as to achieve the purposes of repairing aged and diseased cells and reconstructing functional normal cells and tissues.

The invention relates to a method for inducing mesenchymal stem cells to differentiate into islet-like cells, and aims at providing the method for differentiating the mesenchymal stem cells coming different sources into the islet-like cells to obtain a large number of the islet-like cells. In order to achieve the aim, the method particularly comprises the following steps: 1) carrying out separation and primary culture on the mesenchymal stem cells of umbilical cord, placenta or fat sources; 2) preparing a differentiation medium: preparing BTCM, cell differentiation induced liquid and SCM; 3) inducing the mesenchymal stem cells to differentiate into the islet-like cells in the third stage; 4) obtaining identification of the islet-like cells. The islet-like cells obtained through the method are high in maturity, and less number of the cells can reach a fast hypoglycemic effect, so that the method for inducing the mesenchymal stem cells to differentiate into the islet-like cells has wide application prospects and is applied to the field of biomedicine.

In order to allow differentiation induction of cancer cells into normal cells, the present invention provides an activator of mitochondria containing a component expected to have an epigenetic action, or provides a cell differentiation inducer having a novel composition that has an action to activate mitochondria. The activator of mitochondria contains, as an effective component, 0.03 to 300 μg / mL of a single component of kaempferol, or a mixture component of kaempferol and glycerol. These agents can promote DNA methylation, activate resting genes in cancer cells such as leukemia cells to promote their normal cell division according to the normal cell cycle, and activate mitochondrial functions, to thereby allow continuous differentiation induction into normal cells without arresting the cell differentiation.

The invention relates to a valeriana jatamansi jones breeding method, which is characterized in that the field culture transplanting is carried out after tissue culture breeding or seed breeding; the tissue culture breeding seedling comprises measures including sterile system establishing, culture medium adoption as callus culture, propagation culture and rooting culture, exercising seedling and acclimation seedling treatment, and seedling bed and young seedling disease prevention after the planting; and the seed breeding seedling comprises measures including seed collection time, dormancy stage breaking, bud cell differentiation induction and seedling period disease and pest prevention. The method provided by the invention mainly solves the problem of valeriana jatamansi jones breeding industrialization, the reliable guarantee is provided for the planting, the endangered species are effectively protected, and particularly, the obvious progress is realized in the field of endangered medicinal material rescue.

To provide a screening method for a substance having an anti-obesity action and an anti-obesity drug. A screening method including: a step for contacting a test substance and a synoviolin-gene-expressing cell; and a step for verifying the effect of the test substance on the synoviolin gene expression, or the effect thereof on synoviolin protein activity. An action which reduces the amount of adipose tissue and an action which inhibits induction of adipocyte differentiation are examples of an anti-obesity action. An anti-obesity drug containing, as an active ingredient thereof, an siRNA of synoviolin, a decoy nucleic acid of synoviolin, or an antisense nucleic acid of synoviolin.