9 results about "Cell Differentiation Induction" patented technology

The invention belongs to the technical field of cell culture, and particularly relates to a method for promoting proliferation and differentiation of cervical squamous epithelial cells. The method comprises the following steps: S1, extracting and culturing primary cervix uteri squamous epithelial cells from cervix uteri tissues to obtain primary cervix uteri squamous epithelial cells; s2, adding the primary cervical squamous epithelial cells into a cell proliferation induction culture solution to induce cell proliferation, so as to obtain proliferated cervical squamous epithelial cells; and S3, uniformly mixing the proliferated cervical squamous epithelial cells with differentiation matrigel, and inducing cell differentiation by using a cell differentiation induction culture solution to obtain differentiated cervical squamous epithelial cells. Proliferation and differentiation of the cervical squamous epithelial cells are promoted by preparing a proper culture medium, and influence factors of proliferation and differentiation of the cervical squamous epithelial cells are researched by culturing the cervical squamous epithelial cells in vitro, so that the pathogenesis of cervical erosion and cervical cancer can be clarified; therefore, a direction is provided for research and development of medicines for treating cervical erosion and cervical cancer.

The present invention addresses the problem of providing novel technology for inducing differentiation of stem cells.　The present invention provides a cell differentiation induction method, said cell differentiation induction method comprising a culturing step for culturing stem cells in the presence of extracellular vesicles derived from a tissue specific cell, wherein the tissue specific cell and the cells obtained by differentiation induction are the same type of cells.

To provide methods allowing control of culture conditions that is more robust, in particular less independent from existing knowledge and / or experience, as well as means that can be suitably employed in such methods.SOLUTION: The invention relates to a scaffold for steering cells in a predetermined direction of cell functionality, preferably a cell differentiation scaffold for steering cells in a predetermined direction of cell differentiation. The scaffold comprises a polydimethylsiloxane (PDMS)-, or rubber-, or silicone-based polymeric surface, and one or more cell functionality-inducing stimuli, preferably one or more cell differentiation-inducing stimuli, coupled to the polymeric surface.SELECTED DRAWING: None

To provide a novel differentiation inducer to skeletal muscle Type I cells, which uses a component that was not previously known to induce differentiation to Type I fibers.SOLUTION: A differentiation inducer to skeletal muscle Type I cells includes R-spondin 3.SELECTED DRAWING: None

The invention relates to the technical field of bone marrow mesenchymal stem cell osteogenic differentiation, and particularly discloses a method for inducing bone marrow mesenchymal stem cell osteogenic differentiation, which is technically characterized by comprising the following steps: S1, preparing rat bone marrow mesenchymal stem cells; s2, performing osteogenesis induction; s3, cell differentiation induction: replacing the osteogenic induction culture medium every 2-3 days, continuously culturing for 14-21 days in the culture environment of 37 DEG C and 5% CO2, paying attention to observation of cell morphological change, and detecting the osteogenic differentiation condition of the rat bone marrow mesenchymal stem cells; wherein a mulberry leaf extract is added into the osteogenesis induction culture medium. According to the invention, the mulberry leaf extract is added into the osteogenic induced differentiation culture medium, so that osteogenic differentiation of bone marrow mesenchymal stem cells can be promoted, tissue regeneration is promoted, and the mulberry leaf extract is applied to preparation of the medicine for treating periodontitis; a new experimental basis and a new theoretical basis are provided for research, development and clinical application of the folium mori extract periodontitis treatment medicine.

The present invention addresses the problem of providing a therapeutic agent for frontotemporal lobe degenerative disease (FTLD), a composition for treating FTLD, and a method for treating FTLD, which have not been developed so far. The inventor of the present invention finds that: a prefrontal lobe cerebral cortex neuron is differentiated and induced by an iPS cell derived from an FTLD patient; a therapeutic agent for frontotemporal lobe degenerative disease (FTLD), comprising a compound represented by formula (1-1), a compound represented by formula (2-1), or a compound represented by formula (3-1), a pharmaceutically acceptable salt thereof, or a solvate thereof, is administered to a prefrontal cerebral cortex neuron exhibiting a pathological state of FTLD. Therefore, the pathological state of FTLD of the forehead lobe type cerebral cortical neurons is improved.

This invention provides a method for evaluating stem cell trilineage differentiation based on single-cell membrane tension measurement, comprising: measuring the membrane tension of multiple single cells in a stem cell sample to be tested, and calculating the mean membrane tension; setting a membrane tension threshold for stem cell trilineage differentiation, comparing the mean membrane tension with the threshold, and evaluating the differentiation process and efficiency of the stem cell sample to be tested. This invention also provides the application of the above evaluation method in the quality control of clinical-grade stem cells, the evaluation of stem cell differentiation induction effects, or the screening of stem cell virulence before transplantation. This invention utilizes optical tweezers technology to construct a single-cell mechanical testing platform, enabling non-destructive quantitative measurement of single-cell membrane tension. Cells can remain intact and viable after testing and can be reused. By comparing the membrane tension data of the sample to be tested with the threshold, a systematic evaluation of stem cell differentiation process and efficiency can be completed, which can be widely applied in the fields of stem cell quality control and regenerative medicine clinical translation.

This invention relates to a method for inducing corneal epithelial cells from the differentiation of pluripotent stem cells. More specifically, it relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lines in a serum-free culture medium without the use of feeder cells, and then inducing the resulting ocular surface ectodermal cell lines to differentiate into corneal epithelial cells.

The present invention addresses the problem of providing a method for forming a complex tissue structure and preparing a liver organoid that faithfully reproduces a healthy liver in which a pathological state can be mimicked by a simple process, and further providing a simple method for preparing a steatotic liver disease model organoid in which it is possible to reproduce the degeneration and complex hepatopathy that are specific to steatotic liver disease.　Provided is a method for producing a multilayered liver organoid, said method including: a cocultivation step for cocultivating source cells; a differentiation inducement step for inducing differentiation of the source cells; and a tissue construction step for constructing a tissue structure that is composed of a plurality of layers of cells, wherein the source cells include hepatoblasts, vascular endothelial progenitor cells, and mesenchymal cells.