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Multifunctional intimal stem cell hematopoietic molecular materials and methods

A technology of pluripotent stem cells and molecular materials, applied in the field of biopharmaceuticals, can solve the problems of inability to achieve differentiation induction, high technical requirements, and high differentiation costs, and achieve the effects of low cost of biological research and development, high differentiation efficiency, and high safety

Pending Publication Date: 2022-07-05
深圳医爱健康管理有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] However, most of the existing stem cell differentiation methods use the introduction of KLF4, OCT4, SOX2 and C-MYC four genes to induce differentiation, which not only requires high technical requirements, high differentiation costs, but also has a high failure rate and a high fault tolerance rate, which makes it impossible to achieve large quantities. Differentiation induction is beneficial to stable high-quality cells in the field of biopharmaceuticals, and in order to solve the above technical problems, materials and methods for preparing multifunctional endometrial stem cell hematopoietic molecules are now proposed

Method used

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  • Multifunctional intimal stem cell hematopoietic molecular materials and methods
  • Multifunctional intimal stem cell hematopoietic molecular materials and methods

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Embodiment 1

[0042] Example 1: According to traditional culture methods, multi-functional endometrial stem cell hematopoietic molecular materials and methods, mammalian body fluids were extracted by centrifugation and ultrafiltration to extract somatic cells and injected into bFGF cell proliferation and differentiation regulator with a final concentration of 39ng / ml. Encapsulated in medium No. 1 for culture, senescent or damaged pluripotent hematopoietic stem cells in mammals were used to access medium No. 2 for culture, and the final concentration of 50ng / ml of L-Valley was injected into the medium. Aminoamide reagent, take the osteoblasts or sinusoidal endothelial cells on the surface of the endosteum and trabecular bone of mammals and inject them into culture medium No. 3, and inject insulin with a final concentration of 60ng / ml. Oct4, Sox2, Nanog and The retrovirus of the four transcription factors of LIN28 was transferred into medium No. 4, and the final concentration of 30ng / ml vitami...

Embodiment 2

[0043] Embodiment 2: On the basis of Embodiment 1, the sodium pyruvate solution reagent with a final concentration of 40ng / ml is added dropwise on the basis of the original materials of each culture medium, and then the multifunctional endometrial stem cell hematopoietic molecular material and method are obtained. , take mammalian body fluids, extract somatic cells by centrifugation and ultrafiltration, inject bFGF cell proliferation and differentiation regulator with a final concentration of 39ng / ml, and then seal them into medium No. 1 for culture. Pluripotent hematopoietic stem cells were cultured in culture medium No. 2, and L-glutamine reagent with a final concentration of 50ng / ml and sodium pyruvate solution reagent with a final concentration of 40ng / ml were injected into the medium. The osteoblasts or sinusoidal endothelial cells on the surface of the endosteal and trabecular bone were injected into the medium No. 3, and the final concentration of 60ng / ml of insulin and ...

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Abstract

The invention discloses a multifunctional intimal stem cell hematopoietic molecular material and a method, relates to the field of biological pharmacy, and provides the following scheme aiming at the problems of low cell differentiation induction efficiency, poor cell functionality and the like in the prior art: the multifunctional intimal stem cell hematopoietic molecular material comprises a pluripotent hematopoietic stem cell, a specific gene, a somatic cell, a niche, a lentiviral vector and four transcription factors of Oct4, Sox2, Nanog and LIN28. According to the method provided by the invention, by independently adding the raw materials and the additives into the separated culture medium, the differentiation after the combination of the pluripotent hematopoietic stem cells and the somatic cells under the conditions of constant temperature and normal oxygen concentration can be simulated and realized; and homing, positioning and self-replication of the pluripotent hematopoietic stem cells are induced by utilizing niche obtained on the surfaces of endoosseous membranes and bone trabecula in a marrow cavity of a mammal or from a commercial mammal blood sinus to form and proliferate the APSC pluripoietic stem cells so as to achieve the purposes of repairing aged and diseased cells and reconstructing functional normal cells and tissues.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to multifunctional endometrial stem cell hematopoietic molecular materials and methods. Background technique [0002] Pluripotent stem cells (Pluripotent Stem Cells) are a kind of pluripotent cells with self-renewal and self-replication ability. Under certain conditions, they can differentiate into a variety of APSC pluripotent cells. Pluripotent stem cells (PSC) have the ability to differentiate into a variety of The potential of cells and tissues, but the ability to develop into a complete individual is lost, and the developmental potential is limited to a certain extent. Pluripotent stem cells are the hot spot and focus of current stem cell research. They can differentiate into all cells in the body, and then form all tissues and tissues of the body. Therefore, the study of pluripotent stem cells not only has important theoretical significance, but also has great application val...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N5/0789C12N5/074C12N15/867
CPCC12N5/0696C12N15/86C12N2501/603C12N2501/602C12N2501/608C12N2501/605C12N2740/15043C12N2510/00C12N2800/107C12N2506/11
Inventor 卓成柳李丁
Owner 深圳医爱健康管理有限公司
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