Experimental device and method for high-throughput electrical stimulation to induce cell differentiation and controlled drug release

Abstract

An experimental device for high-throughput electrical stimulation to induce cell differentiation and drug controlled release, the device includes an electrical stimulation signal generator and a cell culture device. The electrical stimulation signal generator is composed of: the minimum system of the single chip microcomputer, the digital-to-analog converter, and the moving ends of the single-pole double-throw switch of the signal conditioning module are connected in sequence; the top of the cell culture device has an upper cover, and the upper cover is connected to the wire. One end is connected to the titanium alloy electrode suspended in the cell culture device, and the other end is connected to the first fixed end of the switch; the bottom plate of the cell culture device is a drug release plate, and the drug release plate is simultaneously connected to the second non-movable end of the switch. The moving end and the ground end of the signal conditioning module are connected; the drug release plate is provided with a drug-loaded conductive polymer film. It can generate electrical stimulation signals of any frequency and amplitude, and can also add drugs at the same time, so as to carry out high-throughput electrical stimulation signals and drug screening in the process of in vitro stem cell differentiation, and provide more extensive and reliable experimental basis for stimulation therapy .

Description

technical field [0001] The invention relates to an experimental device and method for electric stimulation to induce cell differentiation and drug controlled release. Background technique [0002] In tissue engineering, the in vitro functional culture of cells and tissues has become the core of tissue engineering. On the premise of providing a living environment similar to that of cells in vivo, how to induce cells to differentiate in a set direction by controlling external stimuli or drug release , is essential for the three-dimensional culture and functionalization of cells and tissues. In recent years, electrical stimulation therapy has developed rapidly, from a single electrical stimulation of nerves to solve muscle movement problems to regulating stem cell differentiation, initiating limb and spinal cord regeneration, inducing ectopic eyeballs, and controlling tumor growth. [0003] Through the in vitro electrical stimulation cell culture test device, the effect and re...

AI Technical Summary

Problems solved by technology

However, the existing in vitro electrical stimulation cell culture test devices usually provide electrical stimulation signals with a constant voltage of direct current, and it is impossible to add drugs to the culture environment, so that it is impossible to analyze the relationship between alternating current signals of various frequencies and amplitudes and cell growth. Speed, regenerative ability, and the role and relationship of differentiation in a specific direction; it is even more impossible to test and study the synergistic and hindering effects and relationship between AC signals and drugs on cell growth speed, regenerative ability, and differentiation in a specific direction

Holds back further development of electrical stimulation therapy

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