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Functionalized substrate to manipulate cell function and differentiation

a functionalized substrate and cell technology, applied in the field of bioinformatics and biotechnology, can solve the problem of relying primarily on the control of culture conditions

Pending Publication Date: 2022-03-03
KONINKLJIJKE PHILIPS NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a method for controlling cell behavior during cultivation in a way that is less dependent on current knowledge and experience. This allows for the direct determination of whether cells respond to the culture conditions in the expected manner and, thus, enables cell cultivation in a more controllable fashion. The method can be used to facilitate the acquisition of desired cell behavior and minimize deviations from the desired pathway activity, thus guiding cells into the desired direction. The method can also provide quantitative information on the activity of signaling pathways that mediate cell behavior, which can be used to safeguard that the respective pathway(s) is / are indeed active and minimize deviations from the desired cell behavior. Overall, the present invention provides a more robust and effective method for controlling cell behavior during cultivation.

Problems solved by technology

A disadvantage of known techniques, whether carried out in conventional culture systems or microfluidic devices, is that they rely primarily on control of culture conditions.

Method used

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  • Functionalized substrate to manipulate cell function and differentiation
  • Functionalized substrate to manipulate cell function and differentiation
  • Functionalized substrate to manipulate cell function and differentiation

Examples

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Embodiment Construction

[0046]The following embodiments merely illustrate particularly preferred methods and selected aspects in connection therewith. The teaching provided herein may be used for constructing several test methods, test systems and / or test kits, e.g., to produce a cell culture, tissue and / or an organ. The following embodiments are not to be construed as limiting the scope of the present invention.

[0047]The extracellular microenvironment plays a significant role in influencing cellular behavior including maintenance of potency, cellular attachment, proliferation, metabolization, lineage-specific differentiation and remodeling. By manipulating the culture conditions in which cells including stem cells are cultivated, it is possible to influence cell behavior, restrict differentiation pathways and thereby produce cell cultures enriched in lineage-specific precursors, particular tissues or organs in vitro among others. Based on this understanding, one aspect of the invention relates to a substr...

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Abstract

The invention relates to a scaffold for steering cells into a predetermined direction of cell functionality, preferably a cell differentiation scaffold for steering cells into a predetermined direction of cell differentiation. The scaffold comprises a polydimethylsiloxane (PDMS)-, or rubber-, or silicone-based polymeric surface, and one or more cell functionality-inducing stimuli, preferably one or more cell differentiation-inducing stimuli, coupled to the polymeric surface.

Description

FIELD OF THE INVENTION[0001]The present invention generally relates to the field of biotechnology, bioinformatics and related arts. More particularly, the present invention relates to a substrate or scaffold for steering cells into a predetermined direction of cell functionality and also to a culturing unit comprising such substrate or scaffold. The invention also pertains to an in vitro method of producing a cell culture, tissue or organ by steering cells into a predetermined direction of cell functionality. The present invention further relates to a non-transitory storage medium storing instruction that are executable by a digital processing device to perform the aforementioned method. The present invention further relates to a computer program comprising program code means for causing a digital processing device to perform the aforementioned method, when the computer program is run on a digital processing device. The present invention further relates to a system for performing th...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12M1/12C12M3/00C12Q3/00C12M1/00C12M1/42G16B5/20C12N5/071
CPCC12M25/14C12M21/08C12Q3/00C12M23/26C12M23/34C12N2506/45G16B5/20C12N5/068C12N2533/30C12N2537/10C12N2506/02C12M35/08C12N5/0068G01N33/5008C12M25/04C12N2533/52C12N2533/54C12N2533/50C12N2533/40C07K14/4702C12N5/00G16B25/10C12M21/00
Inventor VAN DE STOLPE, ANJABECKERS, LUCAS JOHANNES ANNA MARIA
Owner KONINKLJIJKE PHILIPS NV
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