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Enterobacter ludwig with quinclorac degrading function and application thereof

A technology of Enterobacter ludwigii and Enterobacter dervii is applied in the field of degrading quinclorac, which can solve the problems of adverse effects on growth and quality of flue-cured tobacco, economic loss of tobacco leaf production, etc., and achieve high tolerance and degradation ability , the effect of strong vitality and fast growth

Inactive Publication Date: 2019-08-16
BEIJING HEHE RUNSHENG TECH CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, quinclorac-based pesticide residues in the soil have adverse effects on the growth and quality of flue-cured tobacco, and will bring serious economic losses to tobacco leaf production.

Method used

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  • Enterobacter ludwig with quinclorac degrading function and application thereof
  • Enterobacter ludwig with quinclorac degrading function and application thereof
  • Enterobacter ludwig with quinclorac degrading function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] This example is used to illustrate the tolerance and degradation of Enterobacter ludwig provided by the present invention to quinclorac in water

[0046] The bacterial strain LZ-1 is inserted into the LB liquid culture medium of 10mg / L, 20mg / L, 50mg / L, 100mg / L, and 200mg / L quinclorac after sterilization with the inoculation amount of 1 volume % middle. Cultured at 170rpm, 30±1°C for 24h, the results showed that the strain LZ-1 showed good tolerance to quinclorac, and the strains could grow in different media. The maximum OD value that the bacteria can reach and the degradation rate of quinclorac are shown in Table 1.

Embodiment 2

[0048] This example is used to illustrate the tolerance and degradation of Enterobacter ludwig provided by the present invention to quinclorac in water

[0049] Inoculate bacterial strain LZ-1 with the inoculum of 1 volume % into the sterilized inorganic salt culture of quinclorac with a concentration of 10mg / L, 20mg / L, 50mg / L, 100mg / L, and 200mg / L respectively Base. Cultivate at 170rpm, 30±1°C for 24h. The results showed that the strain LZ-1 showed good tolerance to quinclorac, and the strains could grow in different media. In the case of using quinclorac as the only carbon source, the maximum OD value that the bacteria can achieve and the degradation rate of quinclorac are shown in Table 1.

Embodiment 3

[0057] This embodiment is used to illustrate the degradability of Enterobacter ludwig provided by the present invention to quinclorac in soil

[0058] Bacterial strain LZ-1 is inoculated in 100mL LB liquid culture medium with 1 volume % strain quantity, after 175rmp, 30 ± 1 ℃ under cultivation 24h, add bacterial solution to the contamination of 5kg containing 20mg / kg quinclorac In the soil, stir evenly, cultivate for 7 days, and ensure that the water content in the soil is about 20%, and finally detect the content of quinclorac in the soil, and the results are shown in Table 2.

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Abstract

The present invention discloses Enterobacter ludwigii with preservation No. CGMCC No. 10128. The invention also discloses a composition containing the Enterobacter ludwigii live bacteria described above. The present invention further discloses the application of above Enterobacter ludwigii and compositions in the degradation of quinclorac. The present invention also discloses a method for degrading quinclorac. The method comprises the steps of: under the conditions suitable for Enterobacter ludwigii to survive, adding the above Enterobacter ludwigii or composition into an environment containing quinclorac in order to degrade quinclorac. The Enterobacter ludwigii simply requires the conditions of carbon source, nitrogen source and inorganic salts to survive and grow in an atmosphere containing quinclorac, has the advantages of strong vitality, fast growth, large biomass and high tolerance and degradation ability to quinclorac.

Description

technical field [0001] The present invention relates to a strain of Enterobacter ludwigii (Enterobacter ludwigii), in particular to a strain of Enterobacter ludwigii with the function of degrading quinclorac and its application, and an Enterobacter ludwigii containing the Enterobacter ludwigii Compositions and methods of degrading quinclorac. Background technique [0002] Quinclorac (3,7-dichloro-8-quinoline carboxylic acid) is a highly selective hormone-type herbicide developed by BASF in Germany. It is widely used in many rice-growing countries to control monocots and dicotyledons Leaf weeds, especially barnyardgrass control in rice fields, have good herbicidal effect and long residual period, and are more and more widely used. Due to lack of sufficient understanding of the herbicidal properties of quinclorac, some users blindly apply and increase the dose, resulting in soil residues causing phytotoxicity to the next crop, and within 30 days after the application of quinc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/38
Inventor 过钰梁林森张飞燕卢滇楠张杰刘铮
Owner BEIJING HEHE RUNSHENG TECH CO LTD
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