Periplaneta americana preparation and applications thereof in preparing medicines for treating gastric cancer
A technology of Periplaneta americana and its preparation, which is applied in the preparation of Periplaneta americana and its application in the preparation of drugs for the treatment of gastric cancer, to achieve the effect of inhibiting the proliferation of gastric cancer cells and having a better effect
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Embodiment 1
[0032] Example 1 Periplaneta americana extract of the present invention
[0033] Weigh 1000g of the dried worm body of Periplaneta americana, crush it, pass through an 80-mesh sieve, add 12L of 85% ethanol, and reflux once at 80°C, 75°C, and 70°C for 3 hours, 2 hours, and 1 hour respectively. Filtrate, concentrate the filtrate under reduced pressure to a specific gravity of 1.15 (measured at 65°C), add pure water 12 times the mass of the concentrate to the concentrate, stir at 75°C for 20 minutes, refrigerate at 2°C for 24 hours, absorb the upper layer of grease with filter paper , the filtrate after filtering the lower layer was centrifuged at 13000r / min for 30 minutes, and the supernatant was concentrated under reduced pressure, dried and pulverized to obtain the Periplaneta americana extract.
Embodiment 2
[0034] Example 2 Periplaneta americana extract of the present invention
[0035] Weigh 2000g of the dried worm body of Periplaneta americana, crush it, pass through an 80-mesh sieve, add 20L of 90% ethanol and reflux once at 90°C, 80°C, and 70°C for 3.5 hours, 1.5 hours, and 1.5 hours. Filtrate, concentrate the filtrate under reduced pressure to a specific gravity of 1.10 (measured at 60°C), add pure water 12 times the mass of the concentrate to the concentrate, stir at 70°C for 20 minutes, refrigerate at 2°C for 24 hours, absorb the upper layer of grease with filter paper , the filtrate after filtering the lower layer was centrifuged at 10000r / min for 40 minutes, and the supernatant was concentrated under reduced pressure, dried and pulverized to obtain the Periplaneta americana extract.
Embodiment 3
[0036] Example 3 Periplaneta americana extract of the present invention
[0037] Weigh 1000g of the dried worm body of Periplaneta americana, crush it, pass through an 80-mesh sieve, add 12L of 70% ethanol, and reflux once at 90°C, 85°C, and 75°C for 2 hours, 2 hours, and 1 hour respectively. Filtrate, concentrate the filtrate under reduced pressure to a specific gravity of 1.20 (measured at 60°C), add pure water 12 times the mass of the concentrate to the concentrate, stir at 80°C for 20 minutes, refrigerate at 2°C for 24 hours, absorb the upper layer of grease with filter paper , the filtrate after filtering the lower layer was centrifuged at 15000r / min for 20 minutes, and the supernatant was concentrated under reduced pressure, dried and pulverized to obtain the Periplaneta americana extract.
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