Periplaneta americana preparation and applications thereof in preparing medicines for treating gastric cancer

A technology of Periplaneta americana and its preparation, which is applied in the preparation of Periplaneta americana and its application in the preparation of drugs for the treatment of gastric cancer, to achieve the effect of inhibiting the proliferation of gastric cancer cells and having a better effect

Active Publication Date: 2016-08-24
SICHUAN GOODDOCTOR PANXI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently rare domestic reports on the study of Periplaneta americana on gastric cancer

Method used

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  • Periplaneta americana preparation and applications thereof in preparing medicines for treating gastric cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Periplaneta americana extract of the present invention

[0033] Weigh 1000g of the dried worm body of Periplaneta americana, crush it, pass through an 80-mesh sieve, add 12L of 85% ethanol, and reflux once at 80°C, 75°C, and 70°C for 3 hours, 2 hours, and 1 hour respectively. Filtrate, concentrate the filtrate under reduced pressure to a specific gravity of 1.15 (measured at 65°C), add pure water 12 times the mass of the concentrate to the concentrate, stir at 75°C for 20 minutes, refrigerate at 2°C for 24 hours, absorb the upper layer of grease with filter paper , the filtrate after filtering the lower layer was centrifuged at 13000r / min for 30 minutes, and the supernatant was concentrated under reduced pressure, dried and pulverized to obtain the Periplaneta americana extract.

Embodiment 2

[0034] Example 2 Periplaneta americana extract of the present invention

[0035] Weigh 2000g of the dried worm body of Periplaneta americana, crush it, pass through an 80-mesh sieve, add 20L of 90% ethanol and reflux once at 90°C, 80°C, and 70°C for 3.5 hours, 1.5 hours, and 1.5 hours. Filtrate, concentrate the filtrate under reduced pressure to a specific gravity of 1.10 (measured at 60°C), add pure water 12 times the mass of the concentrate to the concentrate, stir at 70°C for 20 minutes, refrigerate at 2°C for 24 hours, absorb the upper layer of grease with filter paper , the filtrate after filtering the lower layer was centrifuged at 10000r / min for 40 minutes, and the supernatant was concentrated under reduced pressure, dried and pulverized to obtain the Periplaneta americana extract.

Embodiment 3

[0036] Example 3 Periplaneta americana extract of the present invention

[0037] Weigh 1000g of the dried worm body of Periplaneta americana, crush it, pass through an 80-mesh sieve, add 12L of 70% ethanol, and reflux once at 90°C, 85°C, and 75°C for 2 hours, 2 hours, and 1 hour respectively. Filtrate, concentrate the filtrate under reduced pressure to a specific gravity of 1.20 (measured at 60°C), add pure water 12 times the mass of the concentrate to the concentrate, stir at 80°C for 20 minutes, refrigerate at 2°C for 24 hours, absorb the upper layer of grease with filter paper , the filtrate after filtering the lower layer was centrifuged at 15000r / min for 20 minutes, and the supernatant was concentrated under reduced pressure, dried and pulverized to obtain the Periplaneta americana extract.

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PUM

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Abstract

The invention provides a periplaneta americana preparation and applications of the periplaneta americana preparation in preparing medicines for treating gastric cancer. Periplaneta americana is treated by adopting an extraction process or an ultralow-temperature grinding process. The research shows that periplaneta americana extract and periplaneta americana medicinal powder can effectively inhibit the expression of VEGF in gastric cancer cells SGC-7901, and have an obvious inhibiting effect for the proliferation of the gastric cancer cells or gastric cancer tumors. The research further shows that after the periplaneta americana extract and the periplaneta americana medicinal powder are respectively mixed with 5-aza-2'-deoxycytidine for use, the inhibition ratios for the proliferation of the gastric cancer cells SGC-7901 cultured in vitro are high, and are respectively 75.1% and 83.9%; after the periplaneta americana extract and the periplaneta americana medicinal powder are mixed with TNP-470 for use, the tumor inhibition rates for the transplantation tumor of tumor-bearing mice are respectively 75.3% and 86.5%.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicines, in particular to a Periplaneta americana preparation and its application in the preparation of medicines for treating gastric cancer. Background technique [0002] Gastric cancer is one of the most common malignant tumors in my country. Its incidence rate ranks first among all kinds of tumors in my country. About 170,000 people die from gastric cancer every year, which is nearly 1 / 4 of all malignant tumor deaths. More than 10,000 new gastric cancer patients are produced. Gastric cancer is indeed a disease that seriously threatens the health of the people. Gastric cancer can occur at any age, but it is more common in 40-60 years old, and there are more men than women, about 2:1. Gastric cancer can occur in any part of the stomach, but it is more common in the gastric antrum, especially the lesser curvature of the stomach. Untreated life expectancy is about 13 months. In recent years,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P35/00A61K31/7068
Inventor 耿福能沈咏梅
Owner SICHUAN GOODDOCTOR PANXI PHARMA
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