Method for determining effective ingredients in cefalexin and trimethoprim capsules by ASE-HPLC (accelerated solvent extractor and high performance liquid chromatography)

A technology of sporexin and trimethoprim and active ingredients, which is applied in measuring devices, material separation, and analysis of materials, can solve the problems of poor sensitivity of Haemophilus influenzae, and achieve the effects of quality control, accurate quantification, and high detection efficiency

Inactive Publication Date: 2016-08-24
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This product has a good antibacterial effect on Neisseria, but the sensitivity of Haemophilus influenzae to this product is poor. This product has certain antibacterial effect on some Escherichia coli, Proteus mirabilis, Salmonella and Shigella

Method used

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  • Method for determining effective ingredients in cefalexin and trimethoprim capsules by ASE-HPLC (accelerated solvent extractor and high performance liquid chromatography)
  • Method for determining effective ingredients in cefalexin and trimethoprim capsules by ASE-HPLC (accelerated solvent extractor and high performance liquid chromatography)

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Embodiment 1

[0022] A kind of method that ASE-HPLC measures the active ingredient of sphalexin trimethoprim capsule, specifically, is to comprise that described active ingredient is cephalexin and trimethoprim, and this method has included the following steps successively:

[0023] 1) Weigh 1 g of the content of sporexin trimethoprim capsule and place it in a 10mL extraction pool, use 2% formic acid aqueous solution to extract the sample statically at a temperature of 60°C for 3 minutes, repeat the extraction twice, and remove the solvent in the extract after the extraction is completed Evaporate to dryness, transfer the extract to a 20ml volumetric flask, dilute to 20mL with 2% formic acid aqueous solution, draw 1mL into a centrifuge tube with a graphite carbon SPE column, vortex for 2min, centrifuge at 13000r / min for 3min, take the supernatant for high-efficiency liquid phase analysis;

[0024] 2) The parameters are set as follows when the high performance liquid chromatography is analyz...

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Abstract

The invention provides a method for determining effective ingredients in cefalexin and trimethoprim capsules by ASE-HPLC (accelerated solvent extraction and high performance liquid chromatography) and is intended to provide a method for determining effective ingredients in cefalexin and trimethoprim capsules by ASE-HPLC, the method being high in detection sensitivity, reliable in detection result and high in repeatability; the method comprises: weighing 1 g of contents from cefalexin and trimethoprim capsules, placing in a 10 mL extraction tank, statically extracting a sample at 60 DEG C for 3 min with 2% formic acid solution twice, drying a solvent in extract by distilling after extracting, transferring extract to a 20 ml volumetric flask, fixing volume to 20 Ml with 2% formic acid solution, sucking 1 mL into a centrifugal tube, swirling for 2 min, centrifuging at 13000 r / min for 3 min, and subjecting supernate to high performance liquid chromatography; the method belongs to the technical field of chemical detection.

Description

technical field [0001] The invention provides a method for effective components of sphalexin-trimethoprim capsules, specifically, a method for determining the effective components of sphalexin-trimethoprim capsules by ASE-HPLC; it belongs to the technical field of chemical detection. Background technique [0002] Cephalexin belongs to the first-generation cephalosporin, its main components are cephalexin and trimethoprim, its antibacterial spectrum is similar to that of cephalothin, but its antibacterial activity is worse than the latter. Except for Enterococcus and methicillin-resistant Staphylococcus, most strains of Streptococcus pneumoniae, hemolytic Streptococcus, and penicillinase-producing Staphylococcus are sensitive to this product. This product has a good antibacterial effect on Neisseria, but the sensitivity of Haemophilus influenzae to this product is poor. This product has certain antibacterial effect on some Escherichia coli, Proteus mirabilis, Salmonella and S...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027G01N2030/062
Inventor 陈学松张云平罗达龙陈启钊
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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