SSR primer and method for rootstock pumpkin "No. 2 Huang Chenggen" hybrid seed purity identification

Abstract

The invention discloses an SSR primer and method for rootstock pumpkin "No. 2 Huang Chenggen" hybrid seed purity identification. The SSR primer includes a forward primer: 5'-GGCATTTCTGAGAACAGCTT-3' and a reverse primer 5'-ACGTTAGTTATGCTATTTTGTAGGC-3'. The identification method includes: 1) extracting the genomic DNA of rootstock pumpkin seedlings; 2) using the rootstock pumpkin genomic DNA as the template, and using the SSR primer to conduct PCR amplification; 3) carrying out polyacrylamide gel electrophoresis on the amplification product; and 4) analyzing the electrophoresis result to conclude that a single plant having the specific bands of male parent and female parent simultaneously is a true hybrid, and the offspring only having the female parent characteristic band without male parent characteristic band is a false hybrid or self-bred seed. The primer and the method provided by the invention can effectively and rapidly distinguish "No. 2 Huang Chenggen" hybrid seeds and the female parent seeds and male parent seeds thereof, and accurately detect the hybrid seed purity. The method has the advantages of rapidity, accuracy, low cost, and simple operation, etc.

Description

Technical field: [0001] The invention belongs to the field of molecular detection, and in particular relates to a primer and a method for identifying the purity of seeds of a pumpkin hybrid variety "Huang Chenggen No. 2". Background technique: [0002] The quality of seeds is related to the safety of agricultural production, and one of the important indicators to measure the quality of seeds is purity, so it is particularly important to test the purity of seeds in the process of agricultural production and quality supervision. Identifying the purity and authenticity of varieties with morphological markers takes a long time, is limited by seasons, and has poor polymorphism. With the widespread application of DNA molecular marker technology, the identification of purity from the genome level has become an important direction for the identification of seed purity. Currently commonly used molecular marker technologies include AFLP (Amplified fragment length polymorphism), RAPD ...

AI Technical Summary

Problems solved by technology

In order to ensure the promotion of excellent hybrid varieties and produce the greatest economic benefits, SSR primers and methods for accurately identifying the purity of hybrid seeds of pumpkin "Huangchenggen No. 2" were screened out to solve the problem of "Huangchenggen No. The problem of low purity of seed production provides an accurate, stable, fast and practical method for the purity identification of "Huang Chenggen No. 2" hybrid seeds for seed production and operation enterprises

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