Preparation method of strawberry verticillium dahlia spore suspension

Abstract

The invention discloses a preparation method of a strawberry verticillium dahlia spore suspension. The method comprises the following steps of collecting strawberry verticillium dahlia infected plants; performing separation and purification; screening out pathogenic bacteria of the strawberry verticillium wilt; inoculating the separated verticillium dahlia into a PDA culture medium to perform plate culture and activation; taking down an agar block containing activated pathogenic bacteria; adding the agar block into a mixture formed by a liquid VL culture medium and 2.0-percent saccharose for shake culture; performing gauze filtering and filter paper vacuum pumping filtering for removing rest hyphae to obtain filter liquid for use; centrifuging filter liquid; abandoning supernate; and performing resuspension and sedimentation by 2ml of sterile water to obtain the strawberry verticillium dahlia spore suspension. The liquid culture medium inoculation and culture mode is used, so that the full growth all over the whole liquid culture medium can be fast achieved; and the labor consumption and the material consumption are reduced. The preparation method has the advantages that the inoculation amount is small; the spore generation speed is high; the growth is fast; the differences of spore quantities prepared from the same pathogenic bacteria in the same batch and different batches are small; and the method stability is high.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a method for preparing spore suspension of Verticillium dahliae of strawberry. Background technique [0002] Verticillium wilt of strawberry is a refractory soil-borne disease that commonly occurs in strawberry planting areas in my country. Once infected, it is difficult to control with pesticides. The results of continuous identification show that in Changfeng County, Anhui, a famous hometown of strawberries in my country, and Jurong City, an important planting base in Jiangsu Province, the pathogenic bacteria of strawberry verticillium wilt is mainly Fusarium oxysporum. At present, the identification of Verticillium wilt resistance of strawberry varieties generally adopts the identification method of disease gardens, but due to the large difference in the concentration of pathogens between different plots of disease gardens and often affected by the climate, the resistance to ...

AI Technical Summary

Problems solved by technology

In addition, other liquids such as Chapic and YPA medium also generally have the characteristics of less sporulation and excessive mycelium growth.

Especially when a large number of strawberry populations need to be inoculated, it takes a lot of manpower and material resources to prepare a solid plate medium, and on the other hand, it is easy to increase the contamination probability of the solid medium