Preparation method of aflatoxin b1 gold nanowell array immune electrode

Abstract

The invention relates to a preparation method of an aflatoxin B1 gold nanowell array immunoelectrode, which adopts a chemical deposition method to deposit gold on a polycarbonate filter membrane with a die hole diameter of 400-800nm ​​to obtain a gold nanotube array body, which is used in Deposit gold on a polycarbonate filter membrane with a die hole diameter of 80-200nm to obtain a gold nanocolumn array negative, which is then assembled to form a gold nanowell array electrode; drop protein A solution on the surface of the gold nanotube array electrode to form a protein A / gold Nanowell array electrode; then put into unlabeled AFB1 antibody solution to make AFB1 antibody / protein A / gold nanowell array electrode; and then seal to obtain AFB1 immune reaction electrode. The invention is simple to manufacture, has a three-dimensional structure and a large surface area, and effectively avoids the interference of electrochemical response signals caused by different materials; the antibody is firmly and effectively fixed, has stable and reliable performance, and can realize sensitive and rapid determination of AFB1.

Description

technical field [0001] The invention relates to a preparation method of an electrode, in particular to a preparation method of an aflatoxin B1 gold nanowell array immune electrode. Background technique [0002] Aflatoxin B1 (AFB1) is the most toxic class of biological toxins found so far, which can induce mutations, suppress immunity and cause cancer. Rapid, sensitive and accurate analysis is an effective means to avoid and reduce the harm of AFB1. At present, the analysis and detection methods of AFB1, such as high performance liquid chromatography, enzyme-linked immunoassay, radioimmunoassay, gold standard method, immunoaffinity column purification-fluorescence rapid detection technology, etc., have made great progress, but there are still operational problems. Different defects such as high requirements, cumbersome steps, poor sensitivity, high false detection rate, and expensive instruments. [0003] Electrochemical bioimmune electrodes are based on the recognition and...

AI Technical Summary

Problems solved by technology

However, the immune electrodes prepared by the above methods have deficiencies in varying degrees.

(1) The preparation process of electrodes prepared by electrodeposition is complicated, and it is difficult to ensure the length, wall thickness and uniformity of nanotubes; (2) Due to the nanometer size, the exposed nanotubes are easy to break; (3) Regardless of the electrodeposition method It is also a gold nanotube array electrode prepared by chemical deposition. The bottom of the nanotube is open, and the connection part with the surface of the substrate electrode is the surface of the substrate electrode, or an adhesive, and its material is different from that of the gold nanotube wall ( Even if the surface material of the base electrode is gold, the surface state of the two is different due to the different preparation process and the gold tube wall)

Therefore, when the electrode is placed in the solution, the solution will not only contact the wall of the gold nanotube but also the surface of the substrate electrode or the adhesive. The inconsistency between the electrode functionalization and the surface state of the immunomodification will cause the electrochemical response signal to be interfered to a certain extent, thus affecting to the accuracy of AFB1 immunoassay