Cross-scale blood vessel and three-dimensional printing method thereof

A 3D printing, cross-scale technology, applied in the fields of medical science, prosthesis, additive processing, etc., can solve the problems such as the inability to realize the dynamic loading requirements of the vascular mechanism and the inability to meet the strength of the vascular cell mechanics.

Inactive Publication Date: 2016-10-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main purpose of the tubular structure constructed based on the principle of extrusion is to construct the nutrient channel of the solid structure, which cannot realize the dynamic loading requirements required for the investigation of the vascular mechanism.
The real vascular st

Method used

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  • Cross-scale blood vessel and three-dimensional printing method thereof
  • Cross-scale blood vessel and three-dimensional printing method thereof
  • Cross-scale blood vessel and three-dimensional printing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0078] In the 3D printing method of this embodiment, mouse fibroblast L929 was selected as fibroblast, rat aortic smooth muscle cell A7r5 was selected as smooth muscle cell, and HUVEC was selected as human umbilical vein endothelial cell as vascular endothelial cell. The average outer diameter of the hollow fiber formed under this condition was 1113 μm, the inner diameter was 817 μm, and the wall thickness was 148 μm. The diameter of the rotor is 6 mm.

[0079] In order to print the blood vessels of the above structure, the specific implementation steps are as follows:

[0080] (1) Prepare a sodium alginate aqueous solution with a mass concentration of 8%: weigh a certain amount of biochemical grade sodium alginate powder, sterilize it under ultraviolet light for half an hour, and mix the sodium alginate powder and deionized water in proportion , a magnetic stirrer was stirred for 24 hours, the temperature was set at 60° C., and the rotation speed was 120 rpm. After being mi...

Embodiment 2

[0098] Such as Figure 5 As shown, the method of the present invention is used to print cross-scale vascular structures with different shapes. When printing these vascular structures, the difference lies in that the rotational speed of the rotator 7 and the translational speed of the spray head should be changed accordingly according to the shape of the vascular structures, and the rest of the steps are the same as in Embodiment 1.

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Abstract

The invention discloses a cross-scale blood vessel. The cross-scale blood vessel sequentially comprises an inner lining layer, an inner tube wall formed by spirally winding in a hollow fiber form and an outer tube wall formed by spirally winding in a hollow fiber form from inside to outside. The invention also discloses a three-dimensional printing method of the cross-scale blood vessel. The three-dimensional printing method comprises the following steps of performing three-dimensional printing by using an inner layer printing material containing smooth muscle cells, and spirally winding in the hollow fiber form to form the inner tube wall; performing three-dimensional printing by using an outer layer printing material containing fibroblasts, and spirally winding on the inner tube wall in the hollow fiber form to form the outer tube wall; performing curing treatment on the inner tube wall and the outer tube wall, and using an inner lining material containing vascular endothelial cells to from the inner lining layer inside the inner tube wall. A blood vessel structure printed by the three-dimensional printing method disclosed by the invention not only contains three layers of blood vessel cells but also has a macro-micro two-stage flow channel, thereby facilitating the follow-up culture of cells and research on chemical and mechanical stimuli.

Description

technical field [0001] The invention belongs to the technical field of biomanufacturing, and in particular relates to a cross-scale blood vessel structure and a 3D printing method thereof. Background technique [0002] The risk factors of cardiovascular disease in my country continue to increase, the morbidity and mortality of cardiovascular disease remain high, and the burden of cardiovascular disease is increasing, becoming an important public health problem. At present, the pathogenic mechanism of cardiovascular diseases is mainly discussed around animal model experiments and two-dimensional cell culture experiments. The construction of a three-dimensional vascular cell model facilitates a more realistic simulation of the in vivo environment, which can speed up the study of cardiovascular pathogenic mechanisms. [0003] There are currently two methods to simulate the real organ structure through three-dimensional clustering of cells. One is to use traditional tissue engi...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/50B29C67/00B33Y10/00B33Y40/00B33Y80/00
CPCA61L27/3808A61L27/3804A61L27/3826A61L27/507B33Y10/00B33Y40/00B33Y80/00
Inventor 贺永高庆傅建中
Owner ZHEJIANG UNIV
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