A method for measuring skullcapflavone II concentration in blood plasma

A plasma and concentration technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of simple operation, simple pretreatment method and high sensitivity

Inactive Publication Date: 2018-04-03
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are currently no literature reports on the in vivo assay of Skullcapflavone-II

Method used

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  • A method for measuring skullcapflavone II concentration in blood plasma
  • A method for measuring skullcapflavone II concentration in blood plasma
  • A method for measuring skullcapflavone II concentration in blood plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] Example: Determination of Skullcapflavone-II Concentration in Canine Plasma

[0030] 1. Experimental materials and instruments

[0031] skullcapflavone-II: provided by Zelang Pharmaceutical Group (Nanjing, China); new psoralen isoflavone (internal standard): purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China); test water: ultrapure water; methanol : Chromatographically pure (Merck Company).

[0032] Agilent ZORBAX SB-C 18 Column (150mm×2.1mm I.D., 5μm, Agilent Technologies, Wilmington, DE, USA); API4000 LC-MS / MS triple quadrupole mass spectrometer (AB Co., Ltd., USA), chromatographic workstation Analyst 1.4.2; CPA225D electronic analysis Balance (Sartorius Co., Ltd., Germany); WH-2 Micro Vortex Mixer (Shanghai Huxi Analytical Instrument Factory); Milli-Q System Water Purifier (Micropore, Bedford, MA, USA); Biofuge PrimoR freezer High-speed centrifuge (German Heraeus company).

[0033] 2. Liquid conditions ...

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Abstract

The invention discloses a method for measuring the concentration of 5,2`-dihydroxyl, 6,7,8,5`-tetramethoxyflavone (skullcapflavone II) in blood plasma. A certain amount of organic solvent is added to the sample to carry out the liquid-liquid extraction of the drug. After pretreatment, it is separated by a chromatographic column and detected by a mass spectrometer. concentration.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a method for measuring the concentration of 5,2'-dihydroxyl, 6,7,8,5'-tetramethoxyflavone (skullcapflavone II) in plasma. Background technique [0002] Skullcapflavone-II, namely 5,2'-dihydroxy, 6,7,8,5'-tetramethoxyflavone, is a new flavonoid compound isolated from the medicinal plant Scutellaria baicalensis. SKullcapflavone-II has anti-inflammatory, viral and immunomodulatory effects. In addition, many ingredients have the effects of antipruritic, regulating metabolic disorders, neuroprotection, promoting angiogenesis, anti-cancer and anti-HIV. Skullcapflavone-II has multiple biological properties such as putative role for cytotoxicity, effect on histamine release from mast cells, inhibition of plasminogen activator type 1 elevation inhibitor by trypsin and anti-asthmatic airway inflammatory response. [0003] Its structural formula is as follows: [0004] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 刘史佳张农山戴国梁居文政储继红李长印许美娟吴婷张军冯丽雯
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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