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A kind of rapid detection method of benzopyrene in vegetable oil

A detection method, a technology of benzopyrene, which is applied in the field of rapid detection of benzopyrene in vegetable oil, and can solve problems such as low cost

Active Publication Date: 2018-11-09
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In summary, there is currently a lack of a low-cost, fast and accurate method to determine the content of benzopyrene in vegetable oil

Method used

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  • A kind of rapid detection method of benzopyrene in vegetable oil
  • A kind of rapid detection method of benzopyrene in vegetable oil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Absorbance standard curve of benzopyrene toluene solution

[0035] The benzopyrene standard is configured into a series of toluene solutions with a concentration of 0.00061ug / ml, 0.00207ug / ml, 0.0014ug / ml, 0.00373ug / ml, and 0.01047ug / ml respectively. Under the absorbance, draw a standard curve such as figure 1 shown.

[0036] figure 1 The equation of the standard curve fitted in is, R 2 =0.997, indicating that benzopyrene has a linear relationship with absorbance. Additionally, if figure 1 As shown, it can be seen that when the concentration of benzopyrene is from 0.0006 μg / mL to about 0.012 μg / mL, the absorbance is well in line with the linear relationship. Therefore, the concentration of benzopyrene in vegetable oil with a concentration of 0.0006 μg / mL to about 0.012 μg / mL can be tested by an ultraviolet spectrophotometer.

[0037] 2. The result of using neutral alumina adsorption

[0038] Take some sesame oil samples with known benzopyrene concentration, th...

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Abstract

The invention provides a method for rapidly detecting benzopyrene in plant oil. According to the method, the principle of absorbance is utilized, namely, the difference between absorbance values before and after plant oil specifically adsorbs benzopyrene is proportional to the content of benzopyrene; and thus an ultraviolet spectrophotometer is used to quantitatively detect benzopyrene in plant oil. The method is rapid and simple, the cost is low, and the using of massive organic solvent is avoided.

Description

technical field [0001] The invention belongs to the field of chemical detection, and more specifically relates to a method for rapidly detecting benzopyrene in vegetable oil. Background technique [0002] Benzopyrene is a polycyclic aromatic hydrocarbon formed by the fusion of benzene and pyrene. According to different fused positions, there can be two isomers of benzo(a)pyrene and benzo(e)pyrene, the most common benzopyrene is benzo(a)pyrene (if not specified, benzo Pyrene refers to benzo(a)pyrene). Benzopyrene is a carcinogen and mutagen. Among polycyclic aromatic hydrocarbons, benzopyrene is the most polluting and most carcinogenic. It exists widely in the environment and is relatively stable. There is a certain correlation between the content of benzopyrene and other polycyclic aromatic hydrocarbons in the atmosphere, so benzopyrene is generally regarded as a representative of atmospheric carcinogens. [0003] my country's "GB2716-2005 Edible Vegetable Oil Hygienic St...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 侯同刚王建勋王兰咸漠
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI