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A method for directly sowing dendrobium dendrobium seeds on tea trees to cultivate seedlings
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Dendrobium dendrobium seedling cultivation technology, applied in botany equipment and methods, cultivation, soilless cultivation, etc., can solve the problems of immature ecological seedling cultivation technology in the field, simplify the production process of seedlings, reduce the difficulty of cultivation management and The effect of investing and reducing the intensity of collection
Active Publication Date: 2019-03-05
JINGHONG HONGZHEN AGRI TECH CO LTD
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Problems solved by technology
However, the field ecological seedling raising technology about Dendrobium dendrobii in the prior art is still immature
Method used
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Embodiment 1
[0028] 1. Acquisition and preservation of tooth lobe germplasm
[0029] The seeds of Dendrobium dendrobii that are naturally strong and mature but not cracked in the field are collected and preserved for future use. Dendrobium dendrobium seeds used were collected from wild populations in Longling County, Baoshan City, Yunnan Province.
[0030] 2. Preparation of fungal suspension
[0031] 2.1 Production of medium: PDA liquid medium is conventionally used, with an appropriate amount. Put 50ml of each bottle into 70 bottles of 500ml Erlenmeyer flasks, wrap the mouth of the bottle with newspaper after sealing, put it into a sterilizer and sterilize it at 121°C and 115KPa for 20min, and set it aside.
[0032] 2.2 Inoculate the Tulasnella sp. fungus with the preservation number CGMCC No.9551 that has been activated and cultivated for 7 days in the above-mentioned sterilized Erlenmeyer flask containing 50ml of liquid medium, and ferment at 28°C and 150rpm. Cultured for 7 days.
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Embodiment 2
[0045] Repeat Example 1, with the following differences:
[0046] On June 17, 2014, sowing was carried out in the Longpa Ancient Tea Garden of Yanuo Village, Jinuo Township, Jinghong City, Yunnan Province as a test site. Seed-fungus suspension The seed density was 118 / ml, totaling 136 ml.
[0047] Most of the protocorms developed into seedlings 45-50 days after sowing. A total of 34 sowing points were selected for this sowing, 18 of which had seeds germinated to form seedlings, and the group germination rate was 52.94%. A total of 128 seedlings were obtained, and an average of 3.76 seedlings were obtained at each point.
Embodiment 3
[0049] Repeat Example 1, with the following differences:
[0050] On July 5, 2014, sowing was carried out in Yibigu Tea Garden, Yiwu Township, Mengla County, Yunnan Province as a test site. Seed-fungus suspension The seed density is 88 / ml, 180ml in total.
[0051] Most of the protocorms developed into seedlings 45-50 days after sowing. A total of 45 sowing points were selected for this sowing, 10 of which had seeds germinated to form seedlings, and the group germination rate was 22.22%. A total of 49 seedlings were obtained, and an average of 1.09 seedlings were obtained at each point.
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Abstract
The invention discloses a method for directly sowing seeds of dendrobium devonianum and cultivating seedlings on tea trees. The method includes preparing fungus suspension from Tulasnella sp. and uniformly mixing the fungus suspension with the seeds of the dendrobium devonianum to obtain seed-fungus suspension; winding preservative films on each trunk of each tea tree by 1-3 circles, uniformly dispersing and injecting the seed-fungus suspension in gaps between the preservative films and the trunks and forming holes in positions near seed sowing points by means of pricking. The seeds of the dendrobium devonianum are collected from fields. The method has the advantages that seedling production procedures can be simplified, the production cost can be obviously lowered, the seedling quantities can be increased, the survival rate and the growth speeds of the seedlings can be increased after the seedlings return natural environments, and accordingly the seedlings are excellent in environmental adaptability; dendrobium devonianum products can be harvested by local residents while tea leaves are collected, and accordingly income increasing effects can be realized; extra land occupation, cultivation facility construction and chemical fertilizer and pesticide usage can be omitted, only the extremely low cost input for preparing the seed-fungus suspension is required, and accordingly the method has extremely high popularization and application value.
Description
technical field [0001] The invention belongs to the technical field of dendrobium cultivation, and in particular relates to a method for directly sowing seeds of dendrobium dendrobium on tea tree trunks based on symbiotic germination technology to obtain seedlings of dendrobium dendrobium. Background technique [0002] The stems of Dendrobium devonianum contain high polysaccharides. As a traditional Chinese medicinal material, it has extremely high medicinal value. It is widely cultivated in many areas of Yunnan, especially in Longling County, which has become its pillar industry. The existing cultivation mode is mainly intensive planting, mostly in the form of "company + base + farmer", using tissue cultured seedlings as seedlings. Due to the need to build greenhouses, sprinkler irrigation systems, and land rent, the cost of planting remains high. Simultaneously, in the growth process of dendrobium seedlings, in order to promote the rapid growth of dendrobium seedlings, a l...
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