Process method for extracting tannin from dioscorea cirrhosa lour

A process method, the technology of yam yam, which is applied in the field of biological extraction, can solve the problem of low extraction rate of tannin, achieve the effect of shortening the extraction time and increasing the extraction rate

Active Publication Date: 2016-11-09
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few studies on the extraction process of the effective components of Panicum yam. Most of them use the traditional solvent extraction method, and the extraction rate of tannin is not high. At the same time, there are some shortcomings in the optimization process.

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  • Process method for extracting tannin from dioscorea cirrhosa lour
  • Process method for extracting tannin from dioscorea cirrhosa lour
  • Process method for extracting tannin from dioscorea cirrhosa lour

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Weigh 2g of 40 mesh yam powder in a beaker, add 60% acetone at a material-to-liquid ratio of 1:20, set the temperature to 60°C, set the power to 300W, ultrasonically extract for 40 minutes, and filter after cooling to obtain Tannin extract.

[0040] (2) Take 1 mL of the extract and place it in a 100 mL volumetric flask and dilute to volume with methanol. Take 1mL of the diluted extract, add 6mL of 4% vanillin solution and 3mL of concentrated hydrochloric acid in sequence, cover and shake well, and after reacting for 30min in the dark at (25±1)℃, measure its maximum absorption wavelength at 500nm Absorbance, the calculated tannin extraction rate was 17.56%.

[0041] (3) Centrifuge the tannin extract for 10 minutes, take the supernatant, distill under reduced pressure, and dry in vacuum. Take 100 mg of the extract and dissolve it with 1 mL of 95% ethanol. Use Sephadex LH-20 column for separation and purification. First, use 400 mL of ethanol at a flow rate of 24 mL / h. Af...

Embodiment 2

[0043] (1) Weigh 2g of 100-mesh yam powder in a beaker, add 80% acetone at a material-to-liquid ratio of 1:15, set the temperature to 40℃, set the power to 180W, ultrasonically extract for 20 minutes, and filter after cooling to obtain Tannin extract.

[0044] (2) Take 1 mL of the extract and place it in a 100 mL volumetric flask and dilute to volume with methanol. Take 1mL of the diluted extract, add 6mL of 4% vanillin solution and 3mL of concentrated hydrochloric acid in sequence, cover and shake well, and after reacting for 30min in the dark at (25±1)℃, measure its maximum absorption wavelength at 500nm The absorbance, the calculated tannin extraction rate was 20.08%.

[0045] (3) Centrifuge the tannin extract for 10 minutes, take the supernatant, distill under reduced pressure, and dry in vacuum. Take 100 mg of the extract and dissolve it with 1 mL of 95% ethanol. Use Sephadex LH-20 column for separation and purification. First, use 400 mL of ethanol at a flow rate of 24 mL / h....

Embodiment 3

[0047] (1) Weigh 2g of 60 mesh yam powder in a beaker, add 70% acetone at a material-to-liquid ratio of 1:10, set the temperature to 50℃, set the power to 240W, ultrasonically extract for 30 minutes, and filter after cooling to obtain Tannin extract.

[0048] (2) Take 1 mL of the extract and place it in a 100 mL volumetric flask and dilute to volume with methanol. Take 1mL of the diluted extract, add 6mL of 4% vanillin solution and 3mL of concentrated hydrochloric acid in sequence, cover and shake well, and after reacting for 30min in the dark at (25±1)℃, measure its maximum absorption wavelength at 500nm The absorbance, the calculated tannin extraction rate was 31.45%.

[0049] (3) Centrifuge the tannin extract for 10 min, take the supernatant, distill under reduced pressure, and dry in vacuo. Take 100 mg of the extract and dissolve it with 1 mL of 95% ethanol. Use Sephadex LH-20 column for separation and purification. First, use 400 mL of ethanol at a flow rate of 24 mL / h. Afte...

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Abstract

The invention belongs to the technical field of biological extraction, and discloses a process method for extracting tannin from dioscorea cirrhosa lour. The process method comprises: crushing dioscorea cirrhosa lour, adding acetone, heating, carrying out ultrasonic extracting, cooling, filtering to obtain a tannin extraction liquid, purifying, and separating to obtain tannin. According to the present invention, on the basis of the single factor experiment, the response surface method optimization design is performed to obtain the influence degree of various factors on the dioscorea cirrhosa lour tannin extraction rate, wherein the influence degree sequentially is the temperature, the acetone volume fraction and the time from high to low; in the extraction process, the temperature is preferentially determined; the optimal extraction process conditions comprise that the dioscorea cirrhosa lour particle size is 60 mesh, the ratio of the material to the liquid is 1:10, the acetone volume fraction is 74%, the ultrasonic power is 240 W, the extraction time is 31 min, and the extraction temperature is 54 DEG C, wherein the experiment results show that the tannin content determined under the conditions is 32.27%; with the method, the extraction time is shortened, the extraction rate is increased, and the development and the application of the dioscorea cirrhosa lour are easily achieved.

Description

Technical field [0001] The invention belongs to the technical field of biological extraction, and particularly relates to a process method for extracting tannins from yam. Background technique [0002] Dioscorea, Dioscoreaceae, a perennial twining vine wild plant, mainly distributed in my country's Guangdong, Guangxi, Yunnan, Indochina, and the Philippines. The tannins rich in dioscorea are a kind of complex polyphenol compounds that are widely found in plants. They can not only increase color, but also have medicinal values ​​such as hemostasis and astringent, anti-oxidation, lowering blood fat, lowering blood pressure, and anti-tumor. At present, more researches on dioscorea are its application in dyeing and medical treatment. For example, invention CN101046067A discloses a method for dyeing silk with dioscorea extract, and inventions CN104971190A and CN1736457A respectively disclose dioscorea in the preparation of prevention or treatment The application of diabetes and its co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
CPCC07H1/08C07H13/08
Inventor 章莉娟钟曼丽
Owner SOUTH CHINA UNIV OF TECH
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