A method for early sex identification of open-pollination progeny of 'hort 16A' kiwifruit

Abstract

The invention provides a method for realizing early sex identification on open-pollinated progenies of the 'Hort 16A' kiwi fruit, belonging to a method for identifying the sex of plants. According to the method, through the screening for the SNP markers, a primer 2-1 capable of realizing stable amplification and realizing differences among male and female plants of the amplification products is determined; the primer is adopted for carrying out PCR amplification on the open-pollinated progenies of the 'Hort 16A' kiwi fruit with the known sex, the sequences among the male and female plants are compared, and the SNP markers capable of identifying the sex are determined; the male No.6 sample (X6) is taken as a contrast, the plants having the t homozygosis at the 59bp position are female individuals, and the plants having the t / c heterozygosis at the 59bp position are male individuals; the plants having the g homozygosis at the 71bp position are the female individuals, the plants having the g / a heterozygosis at the 71bp position are the male individuals. The method is simple, efficient and rapid, and extremely great convenience is provided for the production practice of the 'Hort 16A' kiwi fruit.

Description

technical field [0001] The invention belongs to genetic breeding of plants, and relates to a method for early identification of sex of plant offspring. Background technique [0002] Actinidia, vines, perennial, mostly dioecious, is a general term for a class of plants. Among the genus Actinidia, the flesh of Actinidia chinensis 'Hort 16A' is golden yellow, rich in unsaturated fatty acids and a large amount of linolenic acid. The flesh is crispy, sweet, refreshing and juicy. 1200mg, is recognized as an excellent variety, widely cultivated in subtropical regions, and its gender performance has three types: female plants, male plants, and monoecious plants. Some female plants can produce vigorous pollen, while some male plants cannot pollinate normally. There are male plants capable of bearing fruit (McNeilage M A. Progress inbreeding hermaphrodite kiwifruit cultivars and understanding the genetics of sex determination [J]. Biologia Plantarum, 1997, 39 (2): 271-280). For seed...

AI Technical Summary

Problems solved by technology

During this period, male plants with little production significance not only increase the cost of cultivation, but also prolong the period of hybrid breeding, improved varieties, fruit acquisition, female plant selection, etc.

Images