A kind of mercury ion detection test strip and using method thereof
A technology for detecting test paper and mercury ions, which is applied in the field of analytical chemistry, can solve the problems of complex pretreatment, poor selectivity, cumbersome operation, etc., and achieve the effect of simple and fast operation, low cost and good selectivity
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Embodiment 1
[0036] Step 1: Mix 100 μL of Cu:Zn-In-S / ZnS quantum dots with 700 μL of isopropanol, centrifuge at 8000 r / min for 10 minutes, redissolve with ultrapure water to the original volume, and then add 300 μL of phosphate buffer to prepare Cu : Zn-In-S / ZnS quantum dot dispersion.
[0037] Step 2: Add 17.5 μL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution with a concentration of 0.80 mg / mL and 12.5 μL with a concentration of 0.80 mg / mL to the above-mentioned quantum dot solution / mL N-hydroxysuccinimide solution, react at room temperature for 40min. Then add 5 μL of mercaptoethanol solution with a concentration of 0.040 mol / L to the mixture, continue to react for 30 minutes, then add 10 μL of streptavidin solution with a concentration of 10 mg / mL, continue to react for 4 hours, and finally add 22.5 μL with a concentration of 0.70 mg / mL glycine solution, stop the reaction after continuing to react for 80min.
[0038]Step 3: Centrifuge the mixed solution prep...
Embodiment 2
[0041] Step 1. Mix 100 μL CdSe quantum dots with 300 μL isopropanol evenly, centrifuge at 4000 r / min for 5 minutes, redissolve to the original volume with ultrapure water, and then add 300 μL phosphate buffer to prepare a CdSe quantum dot dispersion.
[0042] Step 2. Add 17.5 μL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution with a concentration of 0.40 mg / mL and 15 μL of a concentration of 0.60 mg / mL to the above-mentioned quantum dot solution. mL of N-hydroxysuccinimide solution, react at room temperature for 30min. Then add 7.5 μL of mercaptoethanol solution with a concentration of 0.010 mol / L to the mixture, continue to react for 1 min, then add 10 μL of bovine serum albumin solution with a concentration of 1 mg / mL, continue to react for 0.5 h, add 22.5 μL with a concentration of 0.20 mg / mL glycine solution, stop the reaction after continuing to react for 10 minutes.
[0043] Step 3. Centrifuge the mixed solution of the above-mentioned final reac...
Embodiment 3
[0046] Step 1. Mix 100 μL fluorescent carbon nanoparticles with 500 μL isopropanol evenly, centrifuge at 6000 r / min for 7.5 minutes, redissolve with ultrapure water to the original volume, and then add 300 μL phosphate buffer to prepare fluorescent carbon nanoparticle dispersion .
[0047] Step 2: Add 17.5 μL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution with a concentration of 0.60 mg / mL and 13.8 μL with a concentration of 0.70 mg to the above-mentioned quantum dot solution / mL N-hydroxysuccinimide solution, react at room temperature for 23min. Then add 6.3 μL of mercaptoethanol solution with a concentration of 0.025 mol / L to the mixture, continue to react for 15 minutes, then add 10 μL of ovalbumin solution with a concentration of 5 mg / mL, continue to react for 2.5 hours, add 22.5 μL with a concentration of 0.45 mg / mL mL of glycine solution, stop the reaction after continuing to react for 45min.
[0048] Step 3. Centrifuge the mixed solution of th...
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