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Tissue culture method for improving survival rate of bananas

A technology of tissue culture and survival rate, which is applied in the field of plant tissue culture, can solve problems such as improvement of disinfectants, achieve the effects of promoting growth, reducing tissue culture costs, and promoting plant growth

Active Publication Date: 2016-12-14
广西陆川县乌坭坡珍珠番石榴专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Chinese patent CN 105284618A discloses a method for tissue culture of bananas without MS. This invention provides a method for tissue culture of bananas without MS, using cassava alcohol anaerobic fermentation broth to replace the basic medium MS, which can reduce production cost, turning waste into treasure, but this scheme does not improve the disinfectant. Therefore, it is necessary to provide an ideal disinfectant in the current tissue culture, which can not only disinfect the surface of the explant, but also prevent the explant Tissue culture methods that allow invasion while reducing production costs for tissue culture

Method used

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  • Tissue culture method for improving survival rate of bananas
  • Tissue culture method for improving survival rate of bananas
  • Tissue culture method for improving survival rate of bananas

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Spray lime powder on the ground of the cultivation room with closed doors and windows 20 hours before the cultivation, spray concentration in the air and be 75% medical alcohol, and carry out sterilization; select the banana sucking bud without damage by diseases and insect pests to be the explant, long 2cm, diameter 1cm; Rinse the explants with tap water for 9 minutes, remove them and soak them in 75% alcohol solution for 5 minutes, and soak them for 10 minutes in a plant treatment solution with a mass concentration of 5% after the explants are drained (the composition and weight of the plant treatment solution The percentages are as follows: 37% Ganoderma lucidum extract, 10% Polygonum officinalis juice, 17% garlic juice, 20% onion juice, 8% Huoxiang juice, 8% cactus juice); Inoculate the body in the induction medium and carry out the induction culture in the incubator (the induction medium consists of: COD content is 15% cassava alcohol waste liquid of 300mg / L, 2.0g / L...

Embodiment 2

[0035] 22 hours before the cultivation, spray lime powder on the ground of the cultivation room with doors and windows closed, spray concentration in the air and be 75% medical alcohol, and carry out sterilization; select the banana sucking buds without damage by diseases and insect pests as explants, with a length of 3 cm and a diameter of 2 cm; Rinse the explants with tap water for 12min, remove them and soak them in 75% alcohol solution for 7min, after the explants are drained, soak them in 8% plant treatment solution for 20min (composition and weight of the plant treatment solution) The percentages are as follows: 50% Ganoderma lucidum extract, 15% Polygonum officinalis juice, 10% garlic juice, 10% onion juice, 5% Huoxiang juice, 10% cactus juice); Inoculation of the body was carried out in an incubator in an induction medium for induction cultivation (induction medium consists of: 20% cassava alcohol waste liquid with a COD content of 800mg / L, 4.0g / L sucrose solution, 8.0g...

Embodiment 3

[0037] Spray lime powder on the ground of the cultivation room with doors and windows closed 24 hours before the cultivation, and spray the medical alcohol with a concentration of 75% in the air to carry out sterilization; select the banana sucking buds without damage by diseases and insect pests as explants, with a length of 2.5 cm and a diameter of 1.5 cm. cm; Rinse the explants with tap water for 11 minutes, take them out and soak them in 75% alcohol solution for 6 minutes, and after the explants are drained, soak them for 15 minutes in a plant treatment solution with a mass concentration of 5%-8% (plant treatment The composition and percentage by weight of liquid are as follows: 30% Ganoderma lucidum extract, 20% Polygonum officinalis juice, 20% garlic juice, 15% onion juice, 10% Huoxiang juice, 5% cactus juice); The inoculation of the explants after the bacteria is carried out induction culture in the incubator in induction medium (induction medium consists of: COD content...

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Abstract

The invention relates to the field of plant tissue culture, and discloses a tissue culture method for improving the survival rate of bananas. The tissue culture method comprises the following steps: (1) disinfecting a culture room; (2) selecting explants; (3) sterilizing the explants; (4) carrying out initial induced culture; (5) performing subculture; (6) hardening seedlings. The tissue culture method disclosed by the invention uses a plant treatment liquid to disinfect the explants so as to improve the survival rate of the explants in the tissue culture process, and further uses cassava alcohol wastewater to replace the common MS medium, thereby being capable of effectively lowering the tissue culture cost. The invention further provides a tissue culture method which can improve the survival rate of the banana explants and has no damage to the explants, and further can lower the cost.

Description

【Technical field】 [0001] The invention relates to the field of plant tissue culture, in particular to a tissue culture method for improving the survival rate of bananas. 【Background technique】 [0002] Banana belongs to the Musaceae Musa genus for many years, originated in tropical Asia, and is one of the main fresh fruit species in the world. Banana belongs to triploid and has high sterility. The fruit has no seeds and can only be passed on through asexual reproduction. The commonly used means of reproduction is tissue culture. Since the 1960s, plant tissue culture technology has been continuously developed and gradually matured. Although the theoretical research on plant tissue culture has been quite profound, there are still many problems in the technical link. For example, in the tissue culture disinfection process, the commonly used disinfectants are chemical agents, pesticides, ultraviolet rays, etc. , It is very important to master the disinfection time. If the time ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G7/04A01N65/42A01P1/00
CPCA01G7/045A01H4/001A01H4/008A01N65/00A01N65/42A01N2300/00Y02P60/14
Inventor 朱万勇
Owner 广西陆川县乌坭坡珍珠番石榴专业合作社
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