Preparation method of carboxyl magnetic beads for magnetic particle chemiluminescence immunoassay reagent
A technology of chemiluminescence immunity and carboxyl magnetic beads, which is applied in the direction of microsphere preparation, analytical materials, microcapsule preparations, etc., can solve the inconvenient activation rate and coupling rate of immunomagnetic beads, and cannot guarantee the cross-linking batch of magnetic beads Stability, high cost of raw materials for the preparation of magnetic microspheres, etc., to achieve uniform magnetic field response, uniform size, and reduced risk
Active Publication Date: 2018-08-17
JIANGSU ZECEN BIOTECH CO LTD
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Problems solved by technology
The carboxyl magnetic beads prepared by the existing method have poor magnetic field responsiveness, the raw materials for the preparation of magnetic microspheres are expensive, the equipment is complicated, it is not easy to realize control, the process repeatability is poor, the product quality is unstable, and it is not easy to produce in large quantities; the preparation process It is not convenient to control the activation rate and coupling rate of the magnetic beads in the immunomagnetic beads, which increases the risk of cross-linking of the magnetic beads and cannot guarantee the batch stability of the cross-linking of the magnetic beads
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[0026] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.
[0027] 1. A method for preparing carboxyl magnetic beads for magnetic particle chemiluminescence immunoassay reagents, comprising the following steps:
[0028] (1) Prepare 150ml of metal ion solution, (FeCl 3 ·6H 2 (2) and sodium acetate (NaAc) are mixed into metal ion solution wherein the concentration of iron ion is 0.01mol / L with the mol ratio of 1:8, and mechanical stirring is until fully dissolving;
[0029] (2) Carrying out solvothermal reaction at 150-300°C for 4-24h;
[0030] (3) wash the magnetic microspheres with ethanol and water respectively for 3 times;
[0031] (4) Dry at 60° C. for 4 hours to obtain 1.1 g of magnetic microspheres with superparamagn...
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Abstract
The invention discloses a method for preparing carboxyl magnetic beads for a magnetic particle chemiluminescence immune assay reagent. The method comprises the following steps: S1, preparing Fe3O4 nanometer magnetic microspheres; S2, preparing silica magnetic microspheres; S3, aminating the magnetic microspheres; S4, preparing carboxyl magnetic beads; S5, performing magnetic field response screening on the prepared carboxyl magnetic beads. The carboxyl magnetic beads prepared by the method provided by the invention have uniform magnetic field responsiveness, and the magnetic microspheres are cheap and readily available in raw material, simple in equipment, easy to control, high in process repeatability, stable in product quality, safe and reliable in operation, uniform in magnetic field responsiveness and easy for large-scale production. According to the invention, the activating rate and the coupling rate of the magnetic beads can be well controlled in the preparation of immunomagnetic beads, the risk of cross-linking the magnetic beads is reduced, and the stability of batches of magnetic bead coupling is guaranteed.
Description
technical field [0001] The invention relates to a preparation method of carboxyl magnetic beads for magnetic particle chemiluminescence immunoassay reagents. Background technique [0002] Magnetic microspheres are a kind of spherical composite materials with diameters in nanometer or micrometer scale. The core of the magnetic microsphere is composed of superparamagnetic materials such as ferric oxide or ferric oxide, and the outer periphery is coated with polymer materials such as polystyrene or dextran. The core material has superparamagnetism, so that the magnetic beads can move to the direction of the magnetic field under the action of an external magnetic field to achieve the purpose of separation; the peripheral polymer material can be activated by physical or chemical methods to produce amino (NH2-), carboxyl (COOH-) or Groups such as epoxy groups (—CH(O)) can be coupled with biologically active molecules such as proteins. The coupled magnetic beads are widely used i...
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IPC IPC(8): B01J13/02H01F7/02G01N33/531
CPCB01J13/02G01N33/531H01F7/0231
Inventor 郭翠刘振国刘振华程云燕
Owner JIANGSU ZECEN BIOTECH CO LTD
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