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Sample separator and sample separation/adsorption device

A sample and appliance technology, applied in the field of sample separation appliances and sample separation and adsorption devices, can solve the problems of stable detection of biological molecules, unstable transfer state, etc.

Inactive Publication Date: 2016-12-14
SHARP LIFE SCI CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In the technology described in the above-mentioned patent documents, if the contact area of ​​the position where the sample separation part contacts the transfer membrane changes, the state of the transfer becomes unstable, and stable detection of biomolecules becomes difficult.

Method used

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  • Sample separator and sample separation/adsorption device
  • Sample separator and sample separation/adsorption device
  • Sample separator and sample separation/adsorption device

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Experimental program
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no. 1 approach

[0028] Below, refer to figure 1 , figure 2 , the sample separation tool according to the first embodiment of the present invention will be described. In addition, in all of the following drawings, the dimensions, ratios, and the like of each component are suitably different in order to facilitate viewing of the drawings.

[0029] The sample separation device and the sample separation device described below according to the present invention can separate a sample which is a mixture of biomolecules by gel electrophoresis, and are suitable for use in detection operations.

[0030] In addition, in this specification, "biological molecule" refers to a biologically related molecule that participates in a reaction in a living body, and may include a naturally derived molecule, a synthetic molecule, either or both of a naturally derived molecule and a synthetic molecule. any of the complexes.

[0031] Biomolecules to be analyzed are usually macromolecular compounds, preferably bio...

no. 2 approach

[0073] image 3 , 4 It is an explanatory diagram for explaining the sample separation device according to the second embodiment of the present invention, and is a partial cross-sectional view showing the shape of the tip side (discharge port side) of the sample separation device. In the sample separation device according to the second embodiment, the shape of the front end side of the sample separation device is different from that of the sample separation device 1 of the first embodiment. Therefore, the description of components common to the separation tool 1 is omitted.

[0074] image 3 The illustrated sample separator 2 (separator 2 ) is provided with a holding member 31 at the end of the container 10 on the discharge port 10 b side. The holding member 31 has a through-hole 31 a communicating with the discharge port 10 b of the housing portion 10 . The through hole 31a is provided such that the opening diameter L2 on the side opposite to the discharge port 10b is smal...

no. 3 approach

[0081] Figure 5 , 6It is an explanatory diagram for explaining the sample separation and adsorption device 100 according to the third embodiment of the present invention. In the following description, the sample separation and adsorption device 100 may be simply referred to as "separation and adsorption device 100". Figure 5 is a schematic perspective view of the separation and adsorption device 100 . Image 6 It is a cross-sectional view of the separation and adsorption device 100, and is a diagram showing a state in use.

[0082] Such as Figure 5 , 6 As shown, the separation and adsorption device 100 of this embodiment has a sample separation unit 110 and a sample adsorption unit 120 .

[0083] The sample separation unit 110 has a first buffer solution tank 111 , a first electrode 112 , a bridge portion 113 , and the above-mentioned sample separation tool according to the present invention. exist Figure 5 In the diagram, the separation device 1 shown in the first ...

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Abstract

A sample separator (1) comprising an accommodation part (10) that accommodates a separation medium for electrophoresis, and a support (20) formed from a porous material. The accommodation part (10) includes an internal space (10c) filled with the separation medium, and is provided with a supply port (10a) and discharge port (10b) that are in communication with the internal space (10c). The support (20) is provided so as to block the discharge port (10b).

Description

technical field [0001] The invention relates to a sample separation appliance and a sample separation and adsorption device. [0002] This application claims priority based on Japanese Patent Application No. 2014-245857 for which it applied in Japan on December 4, 2014, and uses the content here. Background technique [0003] In molecular biology and biochemistry, individual biomolecules such as proteins and nucleic acids are separated from a sample (sample) containing a plurality of proteins and nucleic acids and analyzed for detection. Polyacrylamide gel electrophoresis (Poly-Acrylamide Gel Electrophoresis) is known as a method for separating individual biomolecules from a sample. PAGE is a method of detecting differences in molecular weights of biomolecules using a gel (separation medium) containing polyacrylamide as a carrier molecule. [0004] In addition, each separated biomolecule is adsorbed and held by a resinous sheet called a transfer film. This operation is so...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/44704G01N27/44739G01N27/44743G01N27/44747G01N27/447G01N33/6803
Inventor 绿川宇一田中毅大木博后藤真一
Owner SHARP LIFE SCI CORP