Folium cortex eucommiae effective position, and preparation method and application thereof

A technology of effective parts and Eucommia leaves, applied in the effective parts of Eucommia leaves, the field of preparation

Inactive Publication Date: 2017-01-04
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological research has found that eucommia leaves have the effects of lowering blood pressure, protecting the liver and diuresis, strengthening bones and muscles, anti-tumor, anti-oxidation, calming abortion, and anti-aging. At present, there are no reports about eucommia leaves protecting the brain nerve damage caused by alcohol

Method used

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  • Folium cortex eucommiae effective position, and preparation method and application thereof
  • Folium cortex eucommiae effective position, and preparation method and application thereof
  • Folium cortex eucommiae effective position, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for preparing the effective part of eucommia leaves, comprising the following steps: after pulverizing 500 g of dried eucommia leaves, ultrasonically extracting them twice with 4000 mL of 40% (v / v, the same below) ethanol respectively, and ultrasonically extracting them for the first time 20min, the second ultrasonic extraction for 30min, filtered, combined two filtrates, concentrated under reduced pressure, adsorbed by HPD100 macroporous adsorption resin, and then washed with water until the effluent was colorless (discarded), and then used 6 times the column volume 20 % ethanol, 6 times the column volume of 50% ethanol for gradient elution, combined the eluent collected by gradient elution, concentrated under reduced pressure to recover the solvent, and dried to obtain 98.8 g of extract, which was the effective part of Eucommia ulmoides leaves.

[0017] Using gallic acid as the reference substance, the total phenolic acid content in the effective parts of Euco...

Embodiment 2

[0019] A method for preparing the effective part of eucommia leaves, comprising the following steps: after pulverizing 200 g of dried eucommia leaves, extracting them twice with 1800 mL of 60% (v / v, the same below) ethanol respectively, and dispersing them for the first time Formula extraction for 3 minutes, the second bulk extraction for 5 minutes, filtration, combined two filtrates, concentrated under reduced pressure, adsorbed by HPD600 macroporous adsorption resin, and then washed with water until the effluent was colorless (discarded), and then sequentially washed with 6 25% ethanol for twice column volume and 55% ethanol for 6 times column volume for gradient elution, and the eluent collected by gradient elution was combined, concentrated under reduced pressure to recover solvent, and dried to obtain 35.2 g of extract, which was the effective part of Eucommia ulmoides leaves.

[0020] Using gallic acid as the reference substance, the total phenolic acid content in Eucommi...

Embodiment 3

[0022] A method for preparing the effective part of Eucommia ulmoides leaves, comprising the following steps: after pulverizing 100 g of dried eucommia leaves, ultrasonically extracting them twice with 1000 mL of 70% (v / v, the same below) ethanol respectively, and ultrasonically extracting them for the first time 20 min, the second ultrasonic extraction for 40 min, filtered, combined two filtrates, concentrated under reduced pressure, adsorbed by D101 macroporous adsorption resin, and then washed with water until the effluent was colorless (discarded), and then used 6 times column Gradient elution with 30% ethanol by volume and 60% ethanol by 6 times the column volume, combined the eluents collected by gradient elution, concentrated under reduced pressure to recover the solvent, and dried to obtain 10.5g of extract, which was the effective part of Eucommia ulmoides leaves.

[0023] Using gallic acid as the reference substance, the content of total phenolic acid in Eucommia ulmo...

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Abstract

The invention relates to a preparation method of a folium cortex eucommiae effective position. The method comprises the following steps of performing ultrasound or scattered extraction on crushed folium cortex eucommiae by 30 to 80-percent ethyl alcohol; after the extraction liquid concentration, performing adsorption by macroporous adsorption resin; then, sequentially performing water washing, 20-60-percent ethyl alcohol gradient elution; merging elution liquid collected in gradient elution; performing concentration and drying to obtain the folium cortex eucommiae effective position. Through experiments, the folium cortex eucommiae effective position has the obvious protection effect on the mouse brain nerve DNA injury induced by alcohol; through the protection mechanism, the anti-oxidization function of the mouse brain tissues can be enhanced; the body is protected from further injury by free radicals; the folium cortex eucommiae effective position can be used for preparing brain nerve protection health care products, food or medicine.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to an effective part of Eucommia ulmoides leaves, a preparation method and its new application in preparing food, health care products and medicines for protecting brain nerve damage caused by alcohol. Background technique [0002] Alcohol abuse is a problem that seriously endangers human health worldwide, causing brain damage, cognitive deficits, loss of motor coordination and corresponding changes in psychological functions. The central nervous system is the main target of ethanol, and its genotoxicity is one of the important mechanisms of ethanol-toxic diseases. At present, it has been proved that the acute and long-term administration of ethanol can damage the DNA of brain cells in different brain regions of mice to varying degrees, and then cause pathological changes such as necrosis, apoptosis, and inflammation of brain nerve cells, and eventually lead to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/46A61P25/00A61P25/28A61P25/32A61P39/06A23L33/105
CPCA61K36/46A23V2002/00A61K2236/333A61K2236/39A61K2236/51A61K2236/55A23V2200/322
Inventor 丛悦郭敬功王江英武彦彤刘习平
Owner HENAN UNIVERSITY
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