Controlled pressurization chromatography system and chromatography method thereof

Abstract

The present invention discloses a controlled pressurization chromatography system and a chromatography method thereof. The system comprises: a liquid storage device provided with a liquid inlet, a gas inlet and a first liquid outlet, wherein the first liquid outlet position is provided with a first flow rate controller and a sample injector; a pressurization device provided with a pressurized gas outlet, wherein the pressurized gas outlet is connected to the gas inlet, and a pressure regulator and a pressure display device are arranged at the pressurized gas outlet position; a buffer device connected to the gas inlet and the pressurized gas outlet; and a chromatography device, wherein a chromatography space is limited inside the chromatography device, a filler layer is arranged inside the chromatography space, the sample injector is embedded into the chromatography space from the upper end of the chromatography device and is positioned above the filler layer, the lower end of the chromatography device is provided with a second liquid outlet, and a second flow rate controller is arranged at the second liquid outlet position. With the technical scheme of the present invention, the pressure controlling during the elution process can be achieved so as to ensure the safety of the experiment; and with the use of the sample injector, the batched adding of the sample to be separated and the eluent can be achieved so as to significantly improve the separation efficiency of the sample to be separated.

Description

technical field [0001] The invention belongs to the field of pharmacy or chemical synthesis, and specifically relates to a controllable pressure chromatography system and a chromatography method. Background technique [0002] Column chromatography is a common separation and purification method in the field of pharmaceutical or chemical synthesis. At present, the common chromatography column is an atmospheric chromatography column. Occasionally, it is manually pressurized with a suction ear ball, and the speed is uncontrollable. The column material used is Ordinary glass, when the filler absorbs solvent and expands, it will make it difficult for the chromatography column to withstand uncontrollable pressure, which will easily cause experimental safety accidents. On the other hand, the chromatographic process requires the use of a large amount of organic solvents, and the solvents in the common chromatographic columns volatilize seriously, which not only causes losses, but als...

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Problems solved by technology

[0002] Column chromatography is a common separation and purification method in the field of pharmaceutical or chemical synthesis. At present, the common chromatography column is an atmospheric chromatography column. Occasionally, it is manually pressurized with a suction ear ball, and the speed is uncontrollable. The column material used is Ordinary glass, when the filler absorbs solvent and expands, it will make it difficult for t

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