Large-scale network array single cell capture microfluidic device
A single-cell, large-scale technology, applied in the field of cell capture, can solve problems such as non-reusability, lower capture efficiency, and device failure, and achieve the effect of reducing the risk of device clogging and improving capture efficiency
Active Publication Date: 2017-01-04
TSINGHUA UNIV
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Problems solved by technology
However, the probability of whether cells can enter the capture unit in the flow channel presents a Gaussian distribution, which reduces the capture efficiency of this method. High-throughput cell inflow is required to ensure the cell capture rate of each unit. In addition, most devices use The serial connection method of the capture unit creates the risk of device failure due to blockage and cannot be reused
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[0025] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary only for explaining the present invention and should not be construed as limiting the present invention.
[0026] In describing the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientations or positional relationships indicated by "vertical", "horizontal", "top", "bottom", "inner" and "outer" are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and Simplified descriptions, rather than indicating or implying that the device or element refe...
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The invention discloses a large-scale network array single cell capture microfluidic device. The microfluidic device comprises a cell capture cavity, wherein a flow inlet and a flow outlet are formed in the cell capture cavity; a cell capture device is arranged in the cell capture cavity and comprises a plurality of single cell capture units; the plurality of single cell capture units are interconnected according to a preset structure to form a preset shape; each single cell capture unit comprises a capture runner and bypass runners; a narrow mouth is formed in the capture runner and is matched with the size of cells to be captured; the capture runner and bypass runners of each single cell capture unit are communicated with the capture runners and bypass runners of adjacent single cell capture units. The microfluidic device has the advantages that the single cell capture units are distributed in a network form, thus achieving a multipath structure during capture; the microfluidic device has adaptability and can achieve the effects of improving the capture efficiency and simultaneously reducing the risk of device blockage.
Description
technical field [0001] The invention relates to the technical field of cell capture, and specifically designs a large-scale network array single cell capture microfluidic device. Background technique [0002] Currently, single cell capture devices in the related art improve throughput by arranging a large number of capture units in a flow channel, or using multiple channels in parallel. When the cell suspension is injected, the cells enter the designed capture unit in a random manner, a small number of cells are confined in the flow channel through narrow openings or grooves in the flow channel, while most of the other cells flow out of the outlet with the suspension. However, the probability of whether cells can enter the capture unit in the flow channel presents a Gaussian distribution, which reduces the capture efficiency of this method. High-throughput cell inflow is required to ensure the cell capture rate of each unit. In addition, most devices use The serial connecti...
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Login to View More IPC IPC(8): C12M1/00
CPCC12M23/16C12M23/34C12M47/04
Inventor王文会米璐黄亮
OwnerTSINGHUA UNIV