Method for producing gamma-polyglutamic acid from activated sludge and straws through fermentation

A technology of polyglutamic acid and activated sludge is applied in the field of bioengineering, which can solve the problem of high cost of fermentation medium and achieve the effect of low treatment cost

Active Publication Date: 2017-01-04
SHANDONG JIANZHU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for producing gamma-polyglutamic acid by fermentation of activated sludge and stra

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] Use the following method to prepare γ-polyglutamic acid:

[0025] 1. The straw is crushed mechanically and sieved through a 20-40 mesh sieve.

[0026] 2. Use a plate and frame filter press to dehydrate the sludge to reduce the moisture content of the sludge cake to below 75% and mix it with straw. The mixing ratio of sludge and straw: straw 30%, activated sludge 70 %.

[0027] 3. Add 1mol / L nitric acid solution until the sludge and straw are completely immersed, and hydrolyze at 70℃ for 3h.

[0028] 4. After the hydrolysate is naturally cooled to room temperature, add 10mol / L sodium hydroxide solution to adjust the pH to 7.0, and centrifuge at 4000r / min to obtain the hydrolysate.

[0029] 5. Add 4% sodium glutamate to each 1000mL hydrolysate to make a fermentation medium, sterilize at 121°C for 15 minutes for later use.

[0030] 6. Inoculate 3% Bacillus subtilis Z-115, and ferment for 80h at 37°C.

[0031] 7. Centrifuge the fermentation broth at 4000r / min for 1...

Example Embodiment

[0032] Example 2

[0033] The method of Example 1 is used to prepare γ-polyglutamic acid. The difference is that the mixing ratio of sludge and straw used in step 2 is 80% straw and 20% activated sludge; the process used in step 3 is: adding 1 mol / L nitric acid solution, until immersed in sludge and straw, hydrolyze at 85 ℃ for 2h. The final yield of γ-polyglutamic acid was 9.4 g / L.

Example Embodiment

[0034] Example 3

[0035] The method of Example 1 was used to prepare γ-polyglutamic acid. The difference is that the process used in step 6 is: inoculate 5% of Bacillus subtilis, ferment for 90h at 37°C, and finally γ-polyglutamic acid The yield is 10.2g / L.

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PUM

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Abstract

The invention provides a method for producing gamma-polyglutamic acid from activated sludge and straws through fermentation. The method comprises the following steps: grinding the straws; carrying out dehydration treatment on sludge, and mixing the dehydrated sludge with the straws; adding nitric acid for hydrolysis; adding alkali for neutralization; preparing a fermentation medium; adding bacillus subtilis for fermentation; and carrying out solid-liquid separation, and extracting gamma-polyglutamic acid in fermentation liquid. The method has the beneficial effects that the technological process is simple, and the operation is convenient; and the environmental pollution caused by the activated sludge and agricultural wastes is reduced, meanwhile, the wastes are recycled, the activated sludge and the straws are subjected to resource utilization so as to produce gamma-polyglutamic acid, and the production cost of gamma-polyglutamic acid is low.

Description

technical field [0001] The invention relates to a method for producing gamma-polyglutamic acid by fermenting activated sludge and straw, which belongs to the field of bioengineering. Background technique [0002] In the sewage treatment process, the activated sludge method is one of the most widely used technologies. This method plays a very important role in water treatment because of its advantages of high efficiency, low cost and less land occupation. However, this method During the process of sewage treatment, a large amount of residual sludge will be produced, usually accounting for 0.5%-1.0% of the total sewage. In an ordinary secondary sewage treatment plant, the investment required for sludge treatment accounts for about 30%-40% of the total investment, and the operating cost accounts for about 20%-50% of the total operating cost. Sludge digestion, dehydration, and then landfill is a commonly used disposal method in domestic large-scale sewage treatment plants. Lan...

Claims

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Application Information

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IPC IPC(8): C12P13/02C12R1/125
Inventor 张超马永山陈文兵赵翔王成菁王琴单蜜蜜
Owner SHANDONG JIANZHU UNIV
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