Application of NK cell stock solution in preparation of medicine for treating skin diseases

A technology of NK cells and skin diseases, applied in skin diseases, animal cells, drug combinations, etc., can solve the problems of single treatment effect, large toxic and side effects, and various types, and achieve the goal of improving the immune system, significant curative effect, and undiminished effect Effect

Inactive Publication Date: 2017-01-25
SHANGHAI MINGDA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art, and to provide an application of NK cell stock solution in the preparation of medicines for treating skin diseases, so as to solve the problems of various kinds of medicines for skin diseases, single treatment effect, long course of treatment, large toxic and side effects, etc. technical problem

Method used

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  • Application of NK cell stock solution in preparation of medicine for treating skin diseases

Examples

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Effect test

Embodiment 1

[0020] 1. Materials

[0021] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0022] 2. Method

[0023] 2.1 Preparation of NK cell stock solution

[0024] Step 1: PBMC extraction:

[0025] Centrifuge the fresh blood at 740g for 10 minutes to obtain the upper layer of light yellow plasma and the lower layer of blood cells, mix the lower layer of blood cells with normal saline at a volume ratio of 1:1, take a 50ml centrifuge tube, and spread the diluted blood cell suspension on 15ml of lymphocyte separation medium Centrifuge at 400g for 30 minutes. Collect the white membranous cell layer in the middle, add physiological saline to wash, centrifuge at 400g for 10 minutes, discard the supernatant, and repeat the washing once. Finally, the cell pellet was resuspended with an appropriate amount of cu...

Embodiment 2

[0058] The present embodiment provides a broad-spectrum external pharmaceutical preparation for the treatment of skin diseases. The pharmaceutical preparation is a SNK solution, which consists of 50% NK cell stock solution, 10.0% glyceryl mono and distearate, ethyl p-hydroxybenzoate 0.5% ester, 0.5% borax, 5.0% dimethyl sulfoxide, 1.0-10.0% sorbic acid and appropriate amount of sterile water.

[0059] Utilize above-mentioned SNK liquid to treat tinea manus, infantile eczema, prickly heat and mosquito bite, after 3 days, symptom alleviates, and can fully recover in 7 days.

Embodiment 3

[0061] A kind of broad-spectrum external pharmaceutical preparation for treating skin diseases provided by the present embodiment, said pharmaceutical preparation is a kind of SNK ointment, consists of 40% of NK cell stock solution, 10.0% of white petrolatum, 10.0% of glyceryl mono and distearate, 0.5% ethyl p-hydroxybenzoate, 0.5% borax, 5.0% dimethyl sulfoxide, 10.0% sorbic acid, and the balance is water.

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Abstract

The invention discloses an application of an NK cell stock solution in preparation of a medicine for treating skin diseases. An NK cell is an important immune cell for an organism, and not only is related to tumor resistance, viral infection resistance and immune regulation, but also takes part in happening of hypersensitive responses and autoimmune diseases in some cases. The NK cell stock solution contains main components of NK cell excreted proteins and cell factors, including MIP-1alpha, MIP-1beta, RANTES, monocyte chemoattractant proteins, interleukin, colony stimulating factors, tumor necrosis factors, interferon and perforin, and these proteins and cell factors can be absorbed by the skin and directly act on target cells to kill or inhibit pathogenic microorganisms including bacteria, fungi, viruses and the like, improve an immune system of the skin, repair the damaged skin, and achieve an aim of comprehensively treating various skin diseases; and after the use of the NK cell stock solution, a protective film is formed on the surface of the skin, which is beneficial for rapid repair of the damaged skin and prevention of repeated aggravation of an illness.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to the application of an NK cell stock solution in the preparation of medicines for treating skin diseases. Background technique [0002] In medicine, dermatosis is a disease related to the skin, and it is one of common diseases and frequently-occurring diseases that seriously affect people's health, such as leprosy, scabies, fungal diseases, skin bacterial infections, etc. Dermatosis is the skin (including hair and nails) affected by internal and external factors, its shape, structure and function all change, resulting in pathological process, and correspondingly produce various clinical manifestations. The incidence rate of skin diseases is very high, most of which are relatively mild, and often do not affect health, but a few of them are severe and even life-threatening. [0003] There are many types of skin diseases, including fungal, bacterial and viral infectious s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/17A61P17/00C12N5/0783
Inventor 王苏鸣王驹于新东
Owner SHANGHAI MINGDA BIOTECH CO LTD
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