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Cell culturing apparatus

A cell culture and culture medium technology, applied in tissue cell/virus culture devices, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve the problems of time and cost, pollution of the culture system, etc. The effect of the risk of systematic errors

Inactive Publication Date: 2017-02-01
OLYMPUS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when the operator enters the operating space, it takes a lot of effort and cost to replace the disposable work clothes, etc.
In addition, the operation process performed by the operator becomes a chance of contamination of the culture system

Method used

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  • Cell culturing apparatus
  • Cell culturing apparatus
  • Cell culturing apparatus

Examples

Experimental program
Comparison scheme
Effect test

no. 1 Embodiment approach

[0036] Hereinafter, a cell culture device according to a first embodiment of the present invention will be described with reference to the drawings.

[0037] The cell culture device 100 of this embodiment is figure 1 The device having the structure shown is a device for replacing the culture medium A in the culture bag (cell culture bag) 2 provided in the incubator.

[0038] The cell culture apparatus 100 includes a culture bag 2 for accommodating cells and a medium A for culturing the cells, a medium holding unit 3, a medium supply unit 4 for supplying the medium A from the medium holding unit 3 to the culture bag 2, and a Medium discharge members 5 and 6 for discharging the medium A from the culture bag 2 .

[0039] The medium holding member 3 is constituted by, for example, a box-shaped container, is arranged inside the incubator 1, and is used to hold the medium (cell culture solution) A inside. The temperature of the medium A is maintained at the temperature inside the...

no. 2 Embodiment approach

[0057] Next, a cell culture device according to a second embodiment of the present invention will be described below with reference to the drawings. The cell culture device 200 of this embodiment is image 3 The device with the configuration shown is different from the first embodiment in that the medium supply unit 4 has a supply speed adjusting member 10 on the tubular member. Other than that, it is the same as the first embodiment.

[0058] The supply speed adjusting part 10 is arranged on the tubular member (pipe, etc.) of the culture medium supply part 4, applies a radial external force to the tubular member to deform it, and restricts the flow rate of the solution by reducing the cross-sectional area of ​​the inner cavity of the tubular member to suppress the flow rate. . On the other hand, when the external force is released, the tubular member returns to its original state due to the elastic force of the tubular member, and the flow rate can be increased. In this wa...

no. 3 Embodiment approach

[0065] Next, a cell culture device according to a third embodiment of the present invention will be described below with reference to the drawings. The cell culture device 300 of this embodiment is Figure 5 The device with the configuration shown is different from the first embodiment in that it has a culture medium holding member 31 and a liquid level sensor 32 inside the culture medium holding member 3 . Other than that, it is the same as the first embodiment.

[0066] The medium storage member 31 has a medium storage container 31a, and is connected to the opening (supply port 3b) of the medium holding member 3 via a tubular member 31c such as a tube. A liquid delivery pump 31d such as a peristaltic pump is provided on the tubular member 31c, and the medium A in the medium storage container 31a can be supplied to the medium holding member 3 by the liquid delivery pump 31d. The medium A in the medium storage container 31a is maintained at a temperature (for example, 4°C) s...

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PUM

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Abstract

A cell culturing apparatus (100) according to the present invention includes a culture-medium retaining means (3) that retains a culture medium (A) for culturing a cell, a culture bag (2) provided with a feed port (2a) and a discharge port (2b), a culture-medium feeding means (4) that connects the culture-medium retaining means (3) and the feed port (2a) and that feeds the culture medium (A) from the culture-medium retaining means (3) to the culture bag (2), a negative-pressure supplying means (6) that supplies negative pressure to the discharge port (2b), and a waste retaining means (5) that retains the culture medium (A) from the discharge port (2b).

Description

technical field [0001] The present invention relates to a cell culture device capable of automatically replacing the culture medium in a culture bag (cell culture bag). Background technique [0002] In recent years, with the development of stem cell research and regenerative medicine, it is required to prepare a large number of cells for clinical use. Preparation of cells for clinical use requires manipulation under strict standards. Therefore, when the operator enters the operating space, it takes a lot of effort and cost to replace the disposable work clothes and the like. In addition, the operation steps performed by the operator may cause contamination of the culture system. Therefore, it is required to reduce the number of times the operator enters the operation space to perform operations as much as possible, and for possible operations, it is required to be performed automatically instead of manually. [0003] When culturing cells, the medium (cell culture solution...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36C12M1/34C12M1/00
CPCC12M23/58C12M29/14C12M29/26C12M41/44C12M41/48C12M45/20C12M23/14C12M29/10C12M33/04C12M41/12C12M41/40C12M29/00C12M41/00C12M41/14
Inventor 木村博之南达哉真柄泰典
Owner OLYMPUS CORP