MiRNA markers for detecting and evaluating effect of strength training or combination thereof and use thereof
A technology for markers and endurance training, applied in the field of miRNA markers or their combinations, can solve the problems of sample preservation, intra-assay and inter-assay variation, lack of muscle damage, poor repeatability, etc., to save cost and time, and avoid complicated detection , easily detectable effects
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Embodiment 1
[0047] Example 1: Specific miRNA expression profile screening of strength training programs
[0048] (1) The research subjects were healthy young males without strength training experience, and all subjects had no neuromuscular, metabolic, hormone-related and cardiovascular diseases. During the whole experiment period, all subjects did not take drugs and maintained similar living and eating habits. The 45 subjects were randomly divided into 3 groups, 15 people in each group (see Table 1 for the details of the volunteers), and they each performed one of the muscle endurance training, muscle hypertrophy training, and muscle maximum strength training programs. Each set of strength training includes 5 movements, namely bench press, half squat, pull-down in sitting position, overhead lift and standing dumbbell curl, and the quiet state before training is used as the control. The subjects were forbidden to perform corresponding strength training after physical exercise for 3 days, ...
Embodiment 2
[0068] Example 2: TaqMan probe Real-time PCR method for measuring miRNA in plasma and determining the miRNA with specific changes as a biomarker for evaluating the effect of strength training
[0069] Reverse transcription was performed using miRNA-specific reverse primers to obtain a cDNA (product of ABI) that contained a common stem-loop structure but belonged to a specific miRNA. The cDNA reverse reaction system of miRNA is: 2 μl RNA, 2 μl 5×AMV buffer, 1 μl 10 mmol of various dNTP mixtures (Takara Company), 0.5 μl AMV (Takara Company) and 1 μl miRNA mature body reverse transcription primer (provided by ABI Company of the United States, dedicated For reverse transcription of miRNA), the volume was made up to 10 μl with 3.5 μl DEPC water. Then Real-time PCR reaction was carried out, and it was found that the two miRNAs (miR-208b and miR-532) had significant differences before and after muscle endurance training ( figure 2 ), six miRNAs (miR-133a, miR-133b, miR-206, miR-21,...
Embodiment 3
[0080] Example 3: Statistical analysis of the relationship between miRNAs specifically changed in plasma after muscle endurance training and traditional marker hormones and muscle damage markers
[0081] In order to further verify the feasibility of the above three miRNAs that specifically change after strength training as biomarkers for evaluating the effect of strength training, correlation analysis was performed between miRNAs and 8 traditional strength training biomarkers. Correlation analysis found that immediately after muscle endurance training, 1 hour and 24 hours after training, miR-532 levels were significantly correlated with IL-10 and IGF-1 ( Figure 5 ). Immediately after muscle maximal strength training, miR-133a levels were significantly correlated with cortisol and testosterone / cortisol values ( Figure 6 ).
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