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Diethylstilbestrol, diethylstilbestrol and hexestrol standard sample in rabbit meat, preparation method and application

A technology of diethylstilbestrol and diethylstilbestrol, applied in the field of analytical chemistry, can solve problems such as affecting the reliability and validity of results, failing to reflect the accuracy of the method, and antioxidants affecting the use results, etc. Easy to store, long shelf life effect

Inactive Publication Date: 2017-02-15
辽宁省检验检疫科学技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the detection of non-steroidal anabolic hormone drug residues in the prior art cannot truly reproduce the extraction efficiency of the drug in animals after natural metabolism, it cannot reflect the accuracy of the method, and affects the reliability and effectiveness of the results. Antioxidants affect the technical problem of use results, an object of the present invention is to provide the preparation method and application of standard samples of diethylstilbestrol, diethylstilbestrol, and hexestrol in rabbit meat

Method used

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  • Diethylstilbestrol, diethylstilbestrol and hexestrol standard sample in rabbit meat, preparation method and application
  • Diethylstilbestrol, diethylstilbestrol and hexestrol standard sample in rabbit meat, preparation method and application
  • Diethylstilbestrol, diethylstilbestrol and hexestrol standard sample in rabbit meat, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of preparation method of diethylstilbestrol, diethylstilbestrol, hexestrol standard sample in rabbit meat, its concrete steps are as follows:

[0034] (1) Selection of experimental animals: Select healthy individual Rex rabbits with a body weight of 2 kg in a rabbit farm, and the sexes are randomized. Isolated at 25°C, fed once a day at 8:00 a.m., domesticated for 3 days and then used for standby, and fasted the day before administration;

[0035] Drug feeding: Weigh 1.93g each of the three drugs (diethylstilbestrol, diethylstilbestrol and hexestrol), and add them to 2.0kg feed at the same time, stir evenly, that is, prepare three kinds of drugs with a concentration of 0.97g / kg. Feed A. Each rabbit was fed 87g of the above-mentioned feed A every day, that is, the dosage of the three drugs was 42mg / kg body weight; once a day, for 5 consecutive days;

[0036] (2) 5 days after the administration, that is, 8 hours after the last administration, take the muscle afte...

Embodiment 2

[0042] In this embodiment, the preparation process in Example 1 is repeated, and the different experimental conditions are:

[0043] 1) The body weight of experimental animals is 2.5kg;

[0044] 2) Weigh 0.2 g each of diethylstilbestrol, diethylstilbestrol and hexestrol;

[0045] 3) The dosage of the three drugs is 3.5 mg / kg body weight;

[0046] 4) Continuous administration for 6 days;

[0047] 5) Slaughter 6 hours after the last administration;

[0048] 6) Pre-freeze at -80°C for 0.5h, vacuum freeze at 0.01mbar for 8h, and quickly heat up and dry for 2h;

[0049] 7) Pass through a 50-mesh sieve;

[0050] 8) Store in cold storage at 5°C.

Embodiment 3

[0052] In this embodiment, the preparation process in Example 1 is repeated, and the different experimental conditions are:

[0053] 1) The body weight of the experimental animals is 1.5kg;

[0054] 2) Weigh 2.0 g each of diethylstilbestrol, diethylstilbestrol and diethylstilbestrol;

[0055] 3) The dosage of the three drugs is 58mg / kg body weight;

[0056] 4) Continuous administration for 3 days;

[0057] 5) Slaughter 12 hours after the last administration;

[0058] 6) Pre-freezing at -30°C for 10 hours, vacuum freezing at 1.0 mbar for 15 hours, and rapid heating and drying for 10 hours;

[0059] 7) pass through 80 mesh sieve;

[0060] 8) Store in cold storage at 8°C.

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Abstract

The invention provides a diethylstilbestrol, diethylstilbestrol and hexestrol standard sample in rabbit meat, a preparation method and application, and belongs to the technical field of analytical chemistry. A rabbit is fed with feedstuff containing diethylstilbestrol, diethylstilbestrol and hexestrol, so that tissues in the body of the rabbit contains the diethylstilbestrol, the diethylstilbestrol and the hexestrol, after administration is finished, the rabbit is killed, and muscles of the rabbit are taken, are homogenized and homogenized, are processed into meat paste, are freeze-dried under vacuum, and are sieved, bottled and packed to obtain a rabbit meat standard sample containing the diethylstilbestrol, the diethylstilbestrol and the hexestrol. By the method, the problems that a target object and matrix combination situation is not completely consistent to a real detecting sample, so that treatment results of extracting, purifying and the like are quite different from daily analysis samples are solved. The standard sample is a material standard sample, is uniform and stable, does not contain a preservative or an antioxidant, is easy to store, and is used for proficiency testing of a non-steroidal anabolic hormones drug residue testing item, verification of a detecting method, calibration of a testing instrument, and quality control and assessment of a testing result.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to the preparation of a natural matrix standard sample of veterinary drug residues, in particular to a standard sample of diethylstilbestrol, diethylstilbestrol, and hexestrol residual matrix freeze-dried powder in rabbit meat and Its preparation method and application. Background technique [0002] Diethylstilbestrol (DES), Hexoestrolum (HEX) and Dienestrol (DS) are all 1,2-stilbene compounds, which are non-steroidal anabolic hormones. All three are colorless crystals or white crystalline powders, easily soluble in methanol, soluble in ethanol, ether or fatty oil, slightly soluble in chloroform, almost insoluble in water; soluble in dilute sodium hydroxide solution . It has the effect of increasing protein deposition and reducing fat deposition, so it is widely used to promote the growth of livestock and poultry, increase lean meat percentage, etc., and diethylstilbestrol has be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28G01N2001/2893
Inventor 王秋艳林维宣王贵滨牛承辉
Owner 辽宁省检验检疫科学技术研究院
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