A method for measuring quercetin-7-glucoside content in sorghum red pigment

A technology for sorghum red pigment and glucoside, which is applied in the field of high performance liquid chromatography for determining the content of quercetin-7-glucoside in sorghum red pigment, which can solve the problem of inappropriateness, cannot fully reflect the content, and has not been detected in sorghum. Red pigment and other problems, to achieve the effect of good reproducibility and reliable stability

Active Publication Date: 2019-12-17
CHANGZHI MEDICAL COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, the evaluation of the quality and performance of sorghum red pigment mainly depends on GB 1886.32-2015 food safety national standard food additive sorghum red color value to evaluate, but this evaluation method is still inappropriate and cannot fully reflect the The content of each component; therefore, it is necessary to quantitatively detect the main components in sorghum red pigment to ensure that each component maintains a certain content in sorghum red pigment
[0006] At present, there is no report on the method for determining the content of quercetin-7-glucoside, the main component of sorghum red pigment

Method used

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  • A method for measuring quercetin-7-glucoside content in sorghum red pigment
  • A method for measuring quercetin-7-glucoside content in sorghum red pigment
  • A method for measuring quercetin-7-glucoside content in sorghum red pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Selection of detection wavelength.

[0035] Use a diode array detector (PDA) to scan the quercetin-7-glucoside standard solution at a full wavelength of 200-800 nm, and obtain a full-wavelength ultraviolet-visible spectral scanning image and a PDA 3D image (see Figure 1~2 ),Depend on figure 1 It can be seen that quercetin-7-glucoside has a maximum absorption at a wavelength of 486 nm, which is determined by figure 2 It can be seen that the quercetin-7-glucoside chromatogram has a stable baseline at 486 nm, less interference and good sensitivity; therefore, 486 nm was selected as the detection wavelength.

Embodiment 2

[0036] Example 2 Selection of mobile phase.

[0037] The mobile phase compositions of different volume ratios were compared and screened, and methanol-water (60:40, V / V), methanol-0.1% acetic acid solution (60:40, V / V), methanol-1% acetic acid solution (60:40, V / V), methanol-1% acetic acid solution (50:50, V / V), methanol-1% acetic acid solution (30:70, V / V) and acetonitrile-1% acetic acid solution ( 40:60, V / V) etc. on quercetin-7-glucoside in C 18 Influence of On-Column Retention Behavior. It was found that when the mobile phase was methanol-1% acetic acid solution (60:40, V / V) as the mobile phase, the peak shape of quercetin-7-glucoside was good, the sample separation was good, and the retention time was moderate ( 7.2 min), see the effect Figure 3~4 .

Embodiment 3

[0038] Example 3 Preparation of standard solution.

[0039] Accurately weigh 10.20 mg of quercetin-7-glucoside standard sample into a 10 ml volumetric flask, add ethanol-hydrochloric acid solution (pH3.0) to dissolve and dilute to the mark, shake well, and make a concentration of 1.02 mg / ml Quercetin-7-glucoside standard stock solution was diluted step by step with ethanol-hydrochloric acid solution (pH3.0) to the required concentration when used.

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Abstract

The invention belongs to the field of analytical chemistry and discloses a high-performance liquid chromatographic analytical method for measuring the content of quercetin-7-glucoside in sorghum red pigment; in the method, octadecylsilane-bonded silica gel is used as a filling agent for a chromatographic column, methanol-acetic acid solution is used as a mobile phase, the content of quercetin-7-glucoside can be quantitatively measured, and the quality of sorghum red pigment is effectively controlled. The method is highly specific and accurate, has good repeatability, and is simple to perform.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a high-performance liquid chromatography method for determining the content of quercetin-7-glucoside (3,5,3',4'-tetrahydroxyflavone-7-glucoside) in sorghum red pigment Methods. Background technique [0002] Sorghum red, also known as sorghum red, sorghum pigment, etc., is a natural pigment extracted from sorghum husk. It is dark reddish brown powder, soluble in water and ethanol aqueous solution, insoluble in petroleum ether and chloroform ; The main components are apigenin (Apigenin; 5,7,4'-trihydroxyflavone) and quercetin-7-glucoside (Quercetin-7-glucoside; 3,5,3',4'-tetrahydroxyflavone- 7-glucoside). Sorghum red pigment has natural, soft, non-toxic, no special smell, low price and good coloring performance. It can be used in various foods according to production needs; and blood pressure functions. [0003] The molecular formula of quercetin-7-glucoside is C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 申科敏胡晓琴
Owner CHANGZHI MEDICAL COLLEGE
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