A dechlorinated edeonella strain and its application
A chloroedeon bacteria and dechlorination technology, applied in the field of microorganisms, can solve problems such as environmental pollution, unfavorable agricultural sustainable development, soil structure damage, etc. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0035] The process of isolation and identification of strains is as follows: the collected fresh root samples of Jujuncao grown in Juncao Institute of Fujian Agriculture and Forestry University were washed with distilled water, and then the roots, stems and leaves were cut off and placed in three sterilized In a petri dish, use 3% hydrogen peroxide (H 2 o 2 ) soaked for 4 minutes to remove rotten substances on the surface of roots, stems and leaves, washed once with sterile water, and then washed with 0.1% mercury chloride (HgCl 2 ) soak for 5 minutes, and vibrate and wash 5 times in sterile water, 5-8 minutes each time. Cut the thoroughly sterilized roots, stems, and leaves with a sterilized scalpel, inoculate them into three test tubes containing 5ml NFB semi-solid medium, seal them with rubber stoppers, and place them in an artificial incubator at 28°C To cultivate. The whole operation is completed under sterile conditions. After the bacteria grow, inject 1 / 10 volume of...
Embodiment 2
[0038] The activated single colonies were cultured in LB liquid culture based on 28°C for 48 hours, and the obtained culture solution OD 600 When the value is 0.6, the preparation concentration is 6×10 8 cfu / mL bacterial solution, with the same volume of clear water inoculated as the control, 15ml of the bacterial solution was poured into each pot of barley, and the bacterial solution was re-irrigated once every 1d. After 15 days, compared with the uninoculated control, the barley leaf length, The root length, fresh weight, and root weight all reached significant differences, and had obvious growth-promoting effects on barley. Among them, the leaf length increased by 26.1%, the root increased by 21.2%, the root weight increased by 48.2.2%, the fresh weight increased by 100.2.%, and the dry weight increased by 63.6.% (Table 1). The effect of promoting barley growth is shown in figure 1 .
[0039] Table 1 Growth-promoting effect of strain FZJG233 inoculated with rice
[0040...
Embodiment 3
[0042] The strain of the invention can be used as a bacteriostatic agent to inhibit the occurrence of rice blast fungus.
[0043] Take 10 μl of the overnight cultured endophytic bacteria droplet in the middle of the PDA medium, cut out the 0.3cm×0.3cm size of the blast pathogen bacterium block cultured for 3-5d on the side of the edge of the PDA medium, and culture it at 28°C for 1-2d , 3 species of Magnaporthe grisea were cultured in confrontation in each petri dish, and each was repeated 3 times. The results were observed after 3-5 days of culture, and the endophyte FZJG233 had an antagonistic effect on rice blast pathogen ( figure 2 ).
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More