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A kind of anti-chicken Newcastle disease virus peptide nucleic acid and its application

A chicken Newcastle disease virus and peptide nucleic acid technology, applied in the field of animal medicine, can solve the problems of unfavorable situation, irregular use of vaccines, huge economic losses, etc., achieve no toxic side effects, inhibit NDV replication, and have good antiviral effect Effect

Active Publication Date: 2017-11-17
南京瑞碧斯生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0018] Despite the widespread use of Newcastle disease vaccines, the current situation is still not optimistic. Clinically, it is mostly atypical Newcastle disease, which causes huge economic losses. At the same time, the use of vaccines is also extremely irregular. Therefore, the poultry breeding industry has strengthened prevention and control NDV appears extremely important

Method used

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  • A kind of anti-chicken Newcastle disease virus peptide nucleic acid and its application
  • A kind of anti-chicken Newcastle disease virus peptide nucleic acid and its application
  • A kind of anti-chicken Newcastle disease virus peptide nucleic acid and its application

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Embodiment Construction

[0035] The antisense nucleic acid sequence designed by the invention

[0036]

[0037] Main materials and reagents

[0038] virus strain

[0039] NDV strain: NS-ND9 strain, provided by the laboratory of Nansen Veterinary Diagnostic Technology Research Center.

[0040] cell line

[0041] CEF cells: 10-day-old SPF chicken embryo fibroblasts (CEF) prepared by conventional methods.

[0042] Synthesis of Antisense Nucleic Acids

[0043] Artificial synthesis of antisense nucleic acid is carried out by synthetic technology.

[0044] The synthesis of antisense nucleic acid In order to obtain good practical application effect, the peptide backbone is used to construct and introduce antisense nucleic acid bases to form peptide nucleic acid (PNA) with stable physical and chemical properties and specific spatial structure

[0045] Modification of Peptide Nucleic Acids

[0046] Peptide nucleic acid was modified with chitosan as a modifier.

[0047] Dosage Forms of Peptide Nucleic...

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Abstract

The invention relates to an anti-chicken newcastle disease virus peptide nucleic acid. Two groups of antisense nucleic acid sequences with high specificity are designed against the highly conserved regions of genes of HN and M proteins of the newcastle disease virus (NDV), can specifically bind to two key genes HN and M of the NDV, are distributed in different regions of an NDV genome, and are reversely complementary to target gene. The reverse nucleic acid sequences specifically bind to the corresponding target gene of the NDV (HN and M) according to the complementary base principle, induce degradation of the target gene by ribozyme and other molecules, block transcription and expression of the target gene and inhibit replication and assembling of the NDV in host in order to control the chicken newcastle disease virus.

Description

technical field [0001] The invention relates to a specific antiviral peptide nucleic acid (antisense oligonucleotide) for preventing and controlling chicken Newcastle disease virus (NDV), in particular to a pharmaceutical composition containing the antisense nucleic acid sequence, and its application in animal medicine the use of. Background technique [0002] Newcastle Disease (ND) is a highly contagious, acute septic avian infectious disease caused by Newcastle Disease Virus (NDV) infection, with respiratory and gastrointestinal mucosal bleeding as typical lesions. The virus mainly infects chickens, guinea fowls and turkeys, etc., and at least more than 200 kinds of birds can be infected. It has become one of the two most easily contagious and devastating diseases that endanger the poultry industry in the world. ND is extremely harmful to infected poultry, so all countries in the world have taken strict precautions against it. It is listed as one of the most harmful Clas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K48/00A61K31/7105A61P31/14
CPCA61K31/7105C12N15/1131C12N2310/3181C12N2320/30C12N2330/00
Inventor 张则斌韩健宝范建华徐步张敬峰徐孝宙马国辅黎先伟王远孝曲向阳
Owner 南京瑞碧斯生物科技有限公司
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