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Methods of tissue culture and fast propagation of Baiji seedlings

A technology of tissue culture rapid propagation and small seedlings, applied in the field of plant seedlings, can solve the problems of low survival rate of tissue culture seedlings domestication

Active Publication Date: 2019-07-12
贵州盛茂白芨开发有限公司
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Problems solved by technology

[0005] Aiming at the technical problem of low domestication and survival rate of existing tissue cultured seedlings, the present invention provides a rapid propagation method for tissue cultured seedlings

Method used

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  • Methods of tissue culture and fast propagation of Baiji seedlings
  • Methods of tissue culture and fast propagation of Baiji seedlings
  • Methods of tissue culture and fast propagation of Baiji seedlings

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Embodiment 1

[0029] The rapid propagation method of bletilla seedling tissue culture comprises the steps:

[0030] Step 1: Preparation of culture soil. After placing the culture soil in a plate, loosen it, put it in a steam pot for sterilization, and then add 50ml of coconut milk and 50ml of potatoes to the culture plate with 1 / 2MS medium formula Juice, 6g agar, configured as a nutrient layer, sterilized by high-temperature steam at 120 degrees for 10 minutes, after cooling naturally, evenly spread a layer of culture soil with a thickness of 8 mm on the medium, then gently compact it and scrape it flat, Then dig a planting pit on the surface of the culture soil, and the bottom of the planting pit is 4mm away from the medium;

[0031] Step 2: explant preparation, select fresh 10,000 Baiji seeds collected in brown pods as tissue culture explants, place Baiji seeds in 70% ethanol solution and soak for 5 minutes, After taking out and draining, use a sealing knife to cut the white and seeds in...

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Abstract

The invention belongs to the field of growing plant seedlings, and particularly discloses a tissue culture and rapid propagation method of bletilla striata seedlings. The method comprises a first step of preparing the culture soil, a second step of preparing the explant, a third step of sowing, a fourth step of cultivating initial seedlings, a fifth step of hardening seedlings initially, a sixth step of hardening seedlings under natural conditions and a seventh step of gaining. At present, a tissue culture and rapid propagation method of bletilla striata seedlings is required badly to solve the technical problem of low domestication survival rate of some existing seedlings.

Description

technical field [0001] The invention relates to the field of plant seedling cultivation, in particular to a method for tissue culture and rapid propagation of bletilla seedlings. Background technique [0002] In recent years, the price of Baiji, a Chinese herbal medicine, has been rising year by year. The transaction price of the four major medicinal materials trading markets has exceeded 500 yuan / kg, and the demand is greater than the supply. Baiji is a terrestrial orchid plant. Baiji is native to China. It is widely distributed in the provinces of the Yangtze River Basin and can be cultivated in most parts of the country. [0003] Due to its own biological characteristics, the natural reproduction coefficient of Baiji is low, and now the method of tissue culture is mainly used to breed Baiji seedlings. Tissue culture propagation with Bletilla seeds is simple in terms of tissue culture technical difficulty. In the past two years, a large number of Bletilla seedlings have a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/25A01C1/00
CPCA01C1/00A01G22/00
Inventor 刘文明
Owner 贵州盛茂白芨开发有限公司
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