Serum-free medium applicable to chondrocyte in vitro culture and preparation method of serum-free medium

Abstract

The invention belongs to the technical field of cell mediums, and in particular relates to a serum-free medium applicable to chondrocyte in vitro culture and a preparation method of the serum-free medium. The medium consists of a basal culture medium and additives, wherein the additives, in terms of final concentration, include the following components: 2-3 [mu]M of butanediamine, 10-20 [mu]g / mL of serine protease inhibitor protein, 15-25ng / mL of fibronectin, 25-35 [mu]g / mL of transferrin, 5-10 [mu]g / mL of insulin, 1-10ng / mL of transforming growth factors, 3-15ng / mL of epidermal growth factors, 2-5mg / mL of polylysine, 1-3 [mu]M of arachidonic acid, 4-10 [mu]M of linolenic acid, 4-11ng / mL of serum spread factors, 15-25 [mu]g / mL of vitamin C, 10-30 [mu]g / mL of parthenolide and 20-32 [mu]g / mL of flavone. The medium provided by the invention is relatively low in cost.

Description

technical field

[0001] The invention belongs to the technical field of cell culture medium, and in particular relates to a serum-free culture medium suitable for culturing chondrocytes in vitro and a preparation method thereof. Background technique

[0002] Articular cartilage is mainly avascular tissue composed of chondrocytes and extracellular matrix. It mainly relies on joint movement and extrusion to absorb nutrients. Therefore, the self-repair ability of cartilage after injury is relatively weak. one of the problems. In recent years, with the development of tissue engineering technology, autologous cartilage transplantation has been applied in the clinical treatment of cartilage injury at home and abroad. Clinical research has limited patient tissues, and because chondrocytes are terminally differentiated cells, their in vitro proliferation ability is limited and they are easy to dedifferentiate, so the cultivation and expansion of in vitro chondrocytes has become the ...

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