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temperature sensitive biosensor

A biosensor and temperature-sensitive technology, applied in the field of bioengineering, can solve the problems of poor temperature sensitivity and insignificant changes in the orientation of liquid crystal droplets, and achieve the effect of high sensitivity and accuracy

Inactive Publication Date: 2018-11-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the liquid crystal droplet temperature sensor has the problem that the orientation change of the liquid crystal droplet is not obvious and the temperature sensitivity is poor.
[0003] Therefore, temperature-sensitive biosensors composed of liquid crystal droplets need to be improved

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0053] A liquid crystal droplet was prepared by using a cell breaker, assembled to form a temperature-sensitive biosensor, and the optical changes of the sensor were observed with a polarizing microscope at a specific temperature.

[0054] (1) Add 10 μL 5CB liquid crystal droplets to 50 μL 20 μM DNA amphiphile DNA-C18, and emulsify with a cell disruptor at 20% power for 30 seconds to prepare liquid crystal droplets. Use a microscope to observe the shape of liquid crystal droplets, such as Figure 4 As shown, the prepared liquid crystal droplets have a large number and relatively uniform size.

[0055] (2) Take 10 μL of the liquid crystal droplet prepared in step (1) and place it in a low-suction PC tube, then add 10 μL of 20 μM DNAlinker (R 1 +R 2 ) solution, let it stand for about 5 minutes and let it self-assemble to form a temperature-sensitive biosensor. Among them, DNA linker (R 1 +R 2 ) has the nucleotide sequence shown in SEQ ID NO: 1 and 2, the melting point T of t...

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Abstract

The invention discloses a temperature sensitive biosensor. The temperature sensitive biosensor comprises liquid crystal molecules, amphiphilic DNA molecules and a connection DNA. Each amphiphilic DNA molecule has a hydrophobic end and a hydrophilic end, and the hydrophobic end is connected with one liquid crystal molecule. Two ends of the connection DNA are connected with the hydrophilic ends of the amphiphilic DNA molecules. The temperature sensitive biosensor can sensitively reflect change in environmental temperature by polymerization and depolymerization of the connection DNA, so that the temperature change can be mastered by observing the state of the liquid crystal droplet.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a temperature-sensitive biosensor. Background technique [0002] The liquid crystal state is a "soft state of matter" that lies between solid crystals and disordered liquids. Liquid crystal molecules have long-range order and optical anisotropy, and have been widely used in the field of flat-panel displays. In 1998, the Abbott research group of the University of Wisconsin in the United States first used liquid crystal molecules to construct biosensors, opening up a new field of liquid crystal applications. Liquid crystal biosensors are easy to miniaturize and array, and have the advantages of simple structure, simple and fast detection, etc., and have shown broad application prospects in the fields of life sciences, clinical medicine and food safety. At present, liquid crystal droplet temperature sensors have the problems of insignificant orientation change of liquid crystal dropl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01K11/00
CPCG01K11/00
Inventor 杨忠强梁晓刘冬生李妍田艺张懿旸
Owner TSINGHUA UNIV