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A method for extracting and detecting active components of lignans in Schisandra chinensis

A technology of lignans and active ingredients, which is applied in the field of extraction and detection of lignans active ingredients in Schisandra chinensis, and achieves the effects of less organic solvent consumption, high resolution, and fast and convenient overall process.

Active Publication Date: 2019-07-26
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report on the separation and detection of lignans in Schisandra chinensis by microemulsion capillary electrokinetic chromatography (MEEKC)

Method used

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  • A method for extracting and detecting active components of lignans in Schisandra chinensis
  • A method for extracting and detecting active components of lignans in Schisandra chinensis
  • A method for extracting and detecting active components of lignans in Schisandra chinensis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The selection of embodiment 1 adsorbent

[0058] Weigh 5 parts of schisandra medicinal material powder (origin Liaoning) each 25mg and add to 5 groups of mortars respectively, and weigh different kinds of adsorbents (molecular sieve TS-1, diatomaceous earth, neutral Al 2 o 3 , silica gel and C 18 ) each 50 mg, respectively added to 5 groups of mortars and grinded with Schisandra powder for 150 s. Take 5 1mL solid-phase extraction columns, add sieve plates to the bottom, fill the extraction columns with ground powder respectively, and add sieve plates to the top after filling the packing. Take 0.5mL of methanol, inject it into the extraction column, use a syringe to properly pressurize the extraction column, and use a 1.5mL centrifuge tube to collect the eluate at the bottom. After the elution, put the centrifuge tube into the centrifuge, 13000rpm Centrifuge for 5 minutes, take the supernatant and get the lignans active ingredient extract (crude drug amount is 50 mg / m...

Embodiment 2

[0064] The selection of embodiment 2 adsorbent consumption

[0065] Weigh 5 portions of schisandra herb powder, 25 mg each, and add them to 5 groups of mortars, and weigh molecular sieve TS-1 with different masses (12.5 mg, 25 mg, 37.5 mg, 50 mg, 75 mg), add them to 5 groups of mortars and schisandra The medicinal material powder is ground for 150s. Take 5 1mL solid-phase extraction columns, add sieve plates to the bottom, fill the extraction columns with ground powder respectively, and add sieve plates to the top after filling the packing. Take 0.5mL of methanol, inject it into the extraction column, use a syringe to properly pressurize the extraction column, and use a 1.5mL centrifuge tube to collect the eluate at the bottom. After the elution, put the centrifuge tube into the centrifuge, 13000rpm Centrifuge for 5 minutes, take the supernatant and get the lignans active ingredient extract (crude drug amount is 50 mg / ml), dilute the extract according to microemulsion: extrac...

Embodiment 3

[0070] Embodiment 3 grinding time selection

[0071] Weigh 5 parts of schisandra medicinal material powder, 25 mg each, and add them to 5 groups of mortars, and weigh 5 parts of 50 mg molecular sieve TS-1, respectively, and add them to 5 groups of mortars for different grinding times (90s, 150s, 180s) . Take 5 1mL solid-phase extraction columns, add sieve plates to the bottom, fill the extraction columns with ground powder respectively, and add sieve plates to the top after filling the packing. Take 0.5mL of methanol, inject it into the extraction column, use a syringe to properly pressurize the extraction column, and use a 1.5mL centrifuge tube to collect the eluate at the bottom. After the elution, put the centrifuge tube into the centrifuge, 13000rpm Centrifuge for 5 minutes, take the supernatant and get the lignans active ingredient extract (crude drug amount is 50mg / ml), microemulsion: extract = 4:1 (v / v) dilute the extract, filter with a 0.22 μm filter membrane Afterwa...

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Abstract

The invention discloses a method for extracting and detecting effective components of lignans in schisandra chinensis. The method includes steps of mixing schisandra chinensis extracting fluid with microemulsion b; filtering the mixture by a 0.22 miu m filter film, and taking the filter fluid as a sample to be tested; detecting by a microemulsion electric capillary chromatography, and obtaining the chromatogram map of the sample to be tested; according to the peak area and the standard curve corresponding to the effective components of the lignans in a chromatogram of the sample to be tested, obtaining the content of the effective components of lignans in the sample to be tested. The matrix solid-phase dispersing extraction technology taking molecular sieve as adsorbent is combined with the microemulsion electric capillary chromatography, and can efficiently and environment-friendly extract and detect contents of effective components of lignans in schisandra chinensis medicinal materials.

Description

[0001] (1) Technical field [0002] The invention relates to a method for analyzing and detecting lignan components in Chinese medicine Schisandra chinensis by using a combined technique of matrix solid phase extraction (MSPD) and capillary electrokinetic chromatography (CE). -1 is used as an adsorbent, combined with matrix solid-phase extraction technology and high-performance capillary chromatography technology, to extract and detect a new method for the content of lignans in Chinese medicine Schisandra chinensis. [0003] (2) Background technology [0004] Schisandra chinensis is the mature fruit of Schisandra chinensis (Turcz.) Baill, a plant of Magnoliaceae. As a blindly tonic drug, it has a long history of medicinal use. It is mainly used for the treatment of chronic cough, dyspnea, nocturnal emission, enuresis, frequent urination, spontaneous sweating, night sweats, thirsty wounds, short Qi and pulse deficiency, internal heat and thirst, heart net insomnia, etc. . Lign...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C41/34C07C41/36C07C43/23C07D317/70C07C43/21G01N30/60
CPCC07C41/34C07C41/36C07D317/70G01N30/60C07C43/23C07C43/21
Inventor 楚楚郑丽琼魏蒙蒙颜继忠
Owner ZHEJIANG UNIV OF TECH
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