Detection method of capsaicin content in emplastrum

A detection method, capsaicin technology, applied in the detection field of capsaicin, can solve the problems such as the inability to evaluate the uniformity of capsaicin, the inability to establish the corresponding relationship of capsaicin, and achieve the effects of high accuracy, high recovery rate and good stability

Inactive Publication Date: 2017-03-29
ZHEJIANG DINGTAI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no specific detection method for the content of capsaicin in the plaster, so it is impossible to establish the corresponding relationship between the content of capsaicin and the efficacy, and it is also impossible to evaluate the uniformity of the content distribution of capsaicin in the plaster during the production process.

Method used

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  • Detection method of capsaicin content in emplastrum
  • Detection method of capsaicin content in emplastrum
  • Detection method of capsaicin content in emplastrum

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Experimental program
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Embodiment

[0017] 1. Prepare the test solution: take three batches of different batches of capsaicin plaster, each with 100cm 2 , cut into narrow strips, removed the lid liner, placed in a stoppered Erlenmeyer flask, precisely added 100ml of methanol-tetrahydrofuran mixed solvent with a volume ratio of 1:1, sealed tightly, weighed, heated and refluxed for 2 hours, and allowed to cool , and then weighed, with a volume ratio of 1:1 methanol-tetrahydrofuran mixed solvent to make up for the lost weight, shake well, and filter to obtain the test solution;

[0018] 2. Prepare the reference solution: take an appropriate amount of capsaicin, weigh it accurately, add methanol-tetrahydrofuran mixed solvent with a volume ratio of 1:1 to make a reference solution containing 50 μg of capsaicin per 1 ml;

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Abstract

The invention relates to the chemical substance content detection technology, and in particular relates to a detection method of the capsaicin. The invention aims at providing the detection method of the capsaicin content in the emplastrum, which has the advantages of simplicity in operation, strong specificity, good reproducibility, and high accuracy. The detection method comprises the following steps: dissolving the capsaicin in the emplastrum by use of a methanol-tetrahydrofuran mixed solvent in volume ratio of 1:1, measuring the capsaicin content by use of the high-performance liquid chromatography, wherein the high-performance liquid chromatography condition is as follows: octadecylsilane chemically bonded silica is taken as a filling agent, and acetonitrile-0.1% phosphoric acid liquid in volume ratio of 46:54 is taken as a mobile phase, and the detection wavelength is 205nm. The detection method disclosed by the invention is strong in specificity and good in repeatability, the RSD of the sample parallel testing is only 1.58%; the linearity is good, the linearity relation with the peak area is good when the capsaicin is between 10microgram / ml to 100microgram / ml; the recovery rate is high, and the accuracy is high.

Description

technical field [0001] The invention relates to a chemical substance content detection technology, in particular to a detection method of capsaicin. Background technique [0002] At present, there are many kinds of plasters on the market, most of which contain capsaicin, which has anti-inflammatory, analgesic, and muscle soreness effects, and its content will have a direct impact on the efficacy of plasters. However, there is currently no specific detection method for the content of capsaicin in the plaster, so it is impossible to establish the corresponding relationship between the content of capsaicin and the drug effect, and it is also impossible to evaluate the uniformity of the content distribution of capsaicin in the plaster during the production process. Therefore, the establishment of an easy-to-operate, reproducible, and high-accuracy detection method for capsaicin content in plasters suitable for enterprises will not only help enterprises to carry out production te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张利华徐建峰
Owner ZHEJIANG DINGTAI PHARMA
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