Method for relieving panax pseudoginseng autotoxin damage by exogenously adding ascorbic acid

A technology of ascorbic acid and self-toxic substances, applied in the field of plant protection, can solve the problems of no public literature reports, continuous cropping obstacles restricting the production of Panax notoginseng, etc., to overcome the decline in oxygen free radical scavenging ability, enhance antioxidant ability, and reduce usage Effect

Inactive Publication Date: 2017-04-26
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Panax notoginseng is one of the most valuable Chinese herbal medicines in my country, but continuous cropping obstacles seriously restrict the production of panax notoginseng
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Method used

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  • Method for relieving panax pseudoginseng autotoxin damage by exogenously adding ascorbic acid
  • Method for relieving panax pseudoginseng autotoxin damage by exogenously adding ascorbic acid
  • Method for relieving panax pseudoginseng autotoxin damage by exogenously adding ascorbic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: utilize exogenous source to add ascorbic acid to strengthen root system cell antioxidant detoxification ability

[0014] After the annual Panax notoginseng seedlings grow in the experimental shade for 7 months, select healthy Panax notoginseng plants and bring them back to the laboratory, rinse the soil on the roots with tap water, rinse them with sterile water three times, and put them into a 200mL tissue culture bottle Perform liquid culture. In order to exclude the influence of 1‰ volume percentage of methanol on the root system of Panax notoginseng, all treatments were added with 1 volume percentage of methanol. A total of 5 treatments were set up in the test, that is, the concentration of 1.0mg / L Rg 1 Treat 0h, 3h, 12h, 24h and 48h respectively. Four repetitions were set up for each treatment, and eight Panax notoginseng seedlings were placed in each repetition. The test adopts the reverse sequence method for treatment. All treatments are sampled a...

Embodiment 2

[0021] Embodiment 2: Utilize ascorbic acid seed soaking treatment to improve the emergence rate of Panax notoginseng

[0022] After the Panax notoginseng seeds are collected, remove the pulp and dry the moisture on the seed surface, and prepare the ascorbic acid solution with a concentration of 0, 0.1, 1.0, 10.0, 50.0, 100.0 mg / L with deionized water with a volume percentage of 0.01% Tween 80 to soak the seeds for 10 minutes , after taking it out, dry the moisture on the surface of the seeds. Then the processed Panax notoginseng seeds are sown in the addition concentration that is 1.0mg / L autotoxic saponin Rg 1 In the soil, the seeds of Panax notoginseng soaked in ascorbic acid were planted in the new soil without adding autotoxic saponin as the control treatment. Three replicates were set up for each treatment, and 1000 seeds were sown in each replicate. After sowing, manage according to conventional methods, and investigate the emergence rate of the control treatment when ...

Embodiment 3

[0027] Embodiment 3: L-ascorbic acid seed coating process promotes the emergence of Panax notoginseng seeds

[0028] After the Panax notoginseng seeds are collected, the moisture on the surface of the seeds is removed from the pulp and dried, and the Shilax seed coating agent containing ascorbic acid concentration of 0, 5.0, 50.0, 500.0, 2500.0, 5000.0 mg / L is used respectively according to the drug seed mass ratio of 1:50. Proportional mixing is performed for coating treatment, and the seed coating agent is dried and then sown. The Panax notoginseng seeds of different treatments were sown in the concentration of 1.0mg / L autotoxic saponin Rg 1In the soil, the Panax notoginseng seeds treated only with the seed coating agent Shilax were planted in the new soil without adding autotoxic saponin as the control treatment. Three replicates were set for each treatment. 1000 seeds were sown per replicate. After sowing, manage according to conventional methods, and investigate the em...

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Abstract

The invention relates to a method for relieving panax pseudoginseng autotoxin damage by exogenously adding an ascorbic acid and belongs to the technical field of plant protection. The concrete technical scheme of the method is as follows: carrying out seed soaking treatment on a panax pseudoginseng seed by using an L-ascorbic acid solution with the concentration of 0.1-50.0mg / L; or dissolving the L-ascorbic acid solution into a seed coating celest to prepare an L-ascorbic acid seed coating with the concentration of 50.0-2500.0mg / L, and then, carrying out coating treatment on the panax pseudoginseng seed; or carrying out root soaking treatment on a seedling by using the L-ascorbic acid solution with the concentration of 0.1-50.0mg / L. The beneficial effects of the method lie in that due to the adoption of the method for exogenously adding the ascorbic acid, the resistance of panax pseudoginseng is improved, the probability of root rot hazards caused by cell injury generated by autotoxins in a panax pseudoginseng growth process is reduced, the use amount of a pesticide is reduced, and the method is a measure for ecologically, safely and effectively relieving continuous cropping obstacles.

Description

technical field [0001] The invention relates to a method for alleviating damage caused by autotoxic substances of Panax notoginseng by adding ascorbic acid from an external source, and belongs to the technical field of plant protection. Background technique [0002] Panax notoginseng is one of the bulk valuable Chinese herbal medicines in my country, but continuous cropping obstacles seriously restrict the production of panax notoginseng. Existing studies have shown that the soil that has been planted with Panax notoginseng cannot be continuously cropped after 10 to 20 years, and the obstacle of continuous cropping has become the main factor restricting the healthy development of the Panax notoginseng industry. Over the years, many scholars have carried out in-depth research on the causes of continuous cropping obstacles of Panax notoginseng, and believe that continuous cropping obstacles are mainly related to the infection of root rot fungi, the accumulation of autotoxic su...

Claims

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Application Information

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IPC IPC(8): A01N43/08A01N25/32A01P21/00A01C1/00A01C1/06A01G1/00
CPCA01N43/08A01C1/00A01C1/06A01G22/00A01N25/32
Inventor 朱书生杨敏钏有聪许彦国刘屹湘黄惠川何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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