Method for producing pinellia-ternate seed stems

A production method, pinellia technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of disease occurrence, soft rot, etc., and achieve the goals of reducing diseases, strong stress resistance, and reducing infestation Effect

Active Publication Date: 2017-05-10
遵义市龙驰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term accumulation of pathogens, artificially

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of pinellia seed stem production method, its steps are as follows:

[0020] 1) Treatment of petiole of explants: select robust petioles of Pinellia lobata with leaves that have not yet unfolded as explants, scrub the surface of petioles of Pinellia pinellia with 75% ethanol, rinse with tap water, and then use 75% (V / V, volume ratio ) soaked in ethanol for 30 s, rinsed with sterilized deionized water for 3 times, and then washed with 0.1% (M / V, volume to mass ratio) HgCl 2 Soak for 8-10 min, rinse with sterilized deionized water for 5 times, and finally dry the surface of the material with sterile filter paper to complete the cleaning and sterilization treatment, and then cut into 0.5-1 cm small pieces.

[0021] 2) In vitro corm induction: Inoculate the cut petiole on the in vitro corm induction medium: 1 / 2MS+6-BA 0.3mg / L+IBA 0.05 mg / L+sugar 3%+agar 0.3%, pH 5.8~ 6.0, 25~27 ℃, illuminance 1600 Lx, light 12h / d, after 100~120 days of cultivation, the medium in the ...

Embodiment 2

[0025] In Example 1, step 2), in vitro corm induction, the medium used was replaced by: 1 / 2MS+6-BA 0.5 mg / L+IBA 0.1 mg / L+white sugar 3%+agar 0.3%, and the rest of the steps were the same .

[0026] It has been proved by experiments that the above-mentioned isolated corm induction medium: 1 / 2MS+6-BA 0.3~0.5 mg / L+IBA 0.05~0.1 mg / L+white sugar 3%+agar 0.3%, is used for the tissue culture of Pinellia petiole to induce The effect is good, after 100-120 days of induced culture, isolated bulbs for greenhouse acclimatization can be obtained. The germ infection rate is small, the production process is simple, the output value is high, and it is suitable for large-scale production.

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Abstract

The invention discloses a method for producing pinellia-ternate seed stems, and belongs to the field of traditional Chinese medicinal material planting. The method includes the steps of explant treating, in-vitro corm induction, greenhouse domestication and the like. Petioles are selected as explant for tissue culturing, pollution is low, the induction effect is good, the induction effect of in-vitro corm induction culturing is good, the petioles are subjected to induction culturing for 100 days to 120 days, and in-vitro corms with buds for greenhouse domestication can be obtained. By means of the method for producing the pinellia-ternate seed stems, it is achieved that the in-vitro corms are obtained through direct induction of the explant, the callus induction process, the embryoid induction process, the rooting induction process are reduced, one step is only needed, and the in-vitro corm induction step is greatly simplified. Soilless culturing is adopted in greenhouse domestication, infection of outside germs is reduced, disease is reduced, and the method has the high resistance. The in-vitro corms are sowed on a net with the certain specification, and the harvesting efficiency is improved. A large amount of high-quality pinellia-ternate seed stems can be harvested after domestication culturing is carried out for one season for supplying large-area cultivation of a field, the whole production technology is simple and easy to operate, operating is easy, and the method is largely suitable for larger-scale production.

Description

technical field [0001] The invention relates to the field of planting Chinese medicinal materials, in particular to a method for producing pinellia seed stems. Background technique [0002] Pinellia is also called Triple Step Tiao, Shou Tian, ​​etc., and belongs to the Araceae family. Widely distributed in the Yangtze River Basin, Northeast China, North China and other regions of China. Medicinal plant, has the functions of drying dampness and resolving phlegm, lowering adverse flow and relieving vomiting, and reducing boils and swelling when used raw. It is used by veterinarians to treat throat lock. [0003] The propagation of pinellia is divided into tuber propagation, bulbil propagation and seed propagation. The male and female inflorescences of Pinellia pinellia are in the same spathe, which is cross-pollination of the same plant. Generally, spathes can only be extracted from larger tubers, and the germination rate of the seeds formed by them is very low under natural...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01H4/00A01G31/00
CPCA01G2/00A01G31/00A01H4/008Y02P60/21
Inventor 刘红美梁大勇方小波石建龙
Owner 遵义市龙驰生物科技有限公司
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