A kind of cultivation method of Sumei black pig
A breeding method and technology for black pigs, applied in animal husbandry and other directions, can solve the problems of accelerated breeding, fertility and adaptability defects, growth rate and lean meat rate and meat quality defects, etc., to achieve the effect of improving pertinence and accuracy
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Embodiment 1
[0026] The traditional breeding of embodiment 1 Samui black pig
[0027] In order to breed a new breed of black pig with high litter performance and strong vitality. Meishan pigs and Berkshire pigs were selected as materials and bred through crossbreeding and group subbreeding. Meishan pigs and Berkshire pigs were selected as parents for crossbreeding to obtain the first generation of Sumei. The individual breeds were relatively pure, and the appearance conformed to the characteristics of each breed. The average litter size of the parents was more than 12, and the body was healthy. Carry out the crossbreeding of Meishan pig and Berkshire pig, each accounting for 50% of blood hybridization. On the basis of conforming to the body shape, the lower part of the limbs is white, and the rest is black, and the number of nipples reaches 8 pairs (more than 16), the selection is based on the body weight at 180 days and the fat thickness at 180 days, and the ratio of male to female is 1....
Embodiment 2
[0053] Embodiment 2 Mismatch PCR-RFLP technology assisted selection
[0054] On the basis of the traditional breeding in Example 1, we used mismatched PCR-RFLP (Dra III) technology and PCR-RFLP (Hinf I) technology to detect two functional genes that affect pig growth traits and lean meat percentage traits, IGF2 Gene, ob gene polymorphism, according to individual gene polymorphism for auxiliary selection. (Note: Insulin-like growth factor 2 (IGF2) plays an important regulatory role in fetal growth and muscle growth, and is the main candidate gene that affects pig lean meat mass; ob gene and its expression product "leptin" (leptin) May reduce feed intake, increase metabolic rate and reduce fat growth.)
[0055] We detected the polymorphism of the known mutation A3072G in intron 3 of the IGF2 gene by using mismatch PCR-RFLP (Dra III) technology. The results showed that 3 genotypes and 2 isotypes were detected at this site. When carrying out molecular markers on the introduced B...
Embodiment 3
[0062] Embodiment 3 establishes BLUP model assisted breeding
[0063] The BLUP model was established on the basis of Examples 1 and 2: this project uses an individual animal model, in which the measured generation and sex are used as fixed effects, individual number is used as an animal effect, and litter number is used as an unrelated random effect.
[0064] Y=Xb+Zu+Sl+e
[0065] Among them, Y is the vector of observed values, b is the vector of fixed effects, u is the vector of random animal effects, l is the vector of litter effects, e is the vector of random residual effects, X, Z, and S are the vectors corresponding to fixed effects, animal effects and Design matrix for the nest effect.
[0066] MTDFREML software was used to estimate the genetic parameters of related traits in Sumei black pigs, and the heritability of the measured traits was between 0.28 and 0.62; are 0.49 and 0.43, which belong to high heritability traits, and the individual selection method is suitabl...
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