A human blood type identification method based on red blood cell optical tweezers Raman spectroscopy

A spectrum technology, optical tweezers Raman technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of long reaction time, dependence of detection results, and low detection accuracy of detection samples, so as to reduce spectral differences and ensure reliability effect

Active Publication Date: 2019-07-23
FUJIAN NORMAL UNIV
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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems existing in the current human blood type differential diagnosis: long reaction time, the need to introduce exogenous monoclonal antibody substances, low detection accuracy for detection samples with low antibody titers, and greatly dependent detection results Based on the subjective judgment of doctors, a human blood type identification method based on red blood cell optical tweezers Raman spectroscopy technology is proposed

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  • A human blood type identification method based on red blood cell optical tweezers Raman spectroscopy
  • A human blood type identification method based on red blood cell optical tweezers Raman spectroscopy
  • A human blood type identification method based on red blood cell optical tweezers Raman spectroscopy

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Embodiment 1

[0035] Concrete technical details of the present invention are set forth as follows according to the implementation to human body ABO blood group identification:

[0036] (1) Preparation of red blood cells to be tested: 30 cases of fresh blood from people with different blood types (A, B, AB, O) were collected, and the blood types of the experimental samples were determined by clinical blood type testing. Mix the obtained fresh blood with PBS buffer, centrifuge, set the centrifugation speed to 1000r / s, centrifuge for 3 minutes, remove the supernatant after centrifugation, add PBS again for centrifugation, repeat this procedure three times to remove other impurities in red blood cells . Finally, the pure activated erythrocytes were evenly mixed with RPMI1640 cell culture medium to make a erythrocyte suspension and placed in the sample pool for testing. Refit the bottom of the petri dish loaded with red blood cells. Specifically, open a small hole with a radius of 1 cm at the b...

Embodiment 2

[0044] (1) Prepare an unknown red blood cell sample to be tested: collect 1 ml of fresh blood from a person with an unknown blood type, mix the obtained fresh blood with PBS buffer, and centrifuge at a speed of 1000 r / s for 3 minutes , remove the supernatant after centrifugation, add PBS again for centrifugation, and repeat this procedure three times to remove other impurities in the red blood cells. Finally, the pure active red blood cells were evenly mixed with RPMI1640 cell culture medium to make a red blood cell suspension in the sample pool for testing.

[0045] (2) Using optical tweezers Raman spectroscopy to test the red blood cell Raman spectrum: using the optical potential well generated by highly converged laser optical tweezers to move a single red blood cell in the sample pool to a relatively independent area, and adjust the objective lens to suspend the red blood cell in the sample At the height of 20-25 μm at the bottom of the pool, perform Raman spectrum detecti...

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Abstract

The invention relates to the technical field of human blood type identification, in particular to a human blood type identifying method based on the erythrocyte laser tweezers Raman spectroscopy. According to the human blood type identifying method, single viable erythrocytes are subjected to Raman spectrum detection by the laser tweezers Raman spectroscopy, so that blood types can be identified rapidly and accurately. The human blood type identifying method specially includes subjecting the viable erythrocytes to real-time Raman spectrum detection by the laser tweezers Raman spectroscopy in an environment close to a human physiological environment so as to obtain Raman spectrum characteristic signals of different blood types of erythrocytes; deeply excavating Raman spectrum characteristic fingerprint information of different blood types of erythrocytes so as to rapidly identify different blood types. The human blood type identifying method has the advantages that the nature of the erythrocytes can be maintained maximally through label-free detection of the viable erythrocytes, and rapidity in detection process and objective, reliable and accurate identification results are achieved; the human blood type identifying method is a novel method for rapid clinical identification of the human blood types.

Description

technical field [0001] The invention relates to the application of laser spectrum technology in medical diagnosis and the field of cell biology. More specifically, the present invention relates to the use of optical tweezers Raman spectroscopy technology to perform Raman spectroscopy detection on individual active red blood cells of different blood types, which can realize rapid and accurate identification of human blood types. Background technique [0002] Human blood types mainly include four types: A, B, AB, and O. Blood of different blood types contains different red blood cells. This difference is mainly manifested in the presence of different antigens on the outer surface of red blood cells (RBC). For example, the outer surface of RBC in type A blood has only antigen A; the outer surface of RBC in type B blood has only antigen B; and the outer surface of RBC in type AB blood has both antigen A and B. In clinical practice, human blood group identification is an extreme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 冯尚源林多郑祖赐王启文翁存程李志华李永增陈荣
Owner FUJIAN NORMAL UNIV
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