A human blood type identification method based on red blood cell optical tweezers Raman spectroscopy
A spectrum technology, optical tweezers Raman technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of long reaction time, dependence of detection results, and low detection accuracy of detection samples, so as to reduce spectral differences and ensure reliability effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0035] Concrete technical details of the present invention are set forth as follows according to the implementation to human body ABO blood group identification:
[0036] (1) Preparation of red blood cells to be tested: 30 cases of fresh blood from people with different blood types (A, B, AB, O) were collected, and the blood types of the experimental samples were determined by clinical blood type testing. Mix the obtained fresh blood with PBS buffer, centrifuge, set the centrifugation speed to 1000r / s, centrifuge for 3 minutes, remove the supernatant after centrifugation, add PBS again for centrifugation, repeat this procedure three times to remove other impurities in red blood cells . Finally, the pure activated erythrocytes were evenly mixed with RPMI1640 cell culture medium to make a erythrocyte suspension and placed in the sample pool for testing. Refit the bottom of the petri dish loaded with red blood cells. Specifically, open a small hole with a radius of 1 cm at the b...
Embodiment 2
[0044] (1) Prepare an unknown red blood cell sample to be tested: collect 1 ml of fresh blood from a person with an unknown blood type, mix the obtained fresh blood with PBS buffer, and centrifuge at a speed of 1000 r / s for 3 minutes , remove the supernatant after centrifugation, add PBS again for centrifugation, and repeat this procedure three times to remove other impurities in the red blood cells. Finally, the pure active red blood cells were evenly mixed with RPMI1640 cell culture medium to make a red blood cell suspension in the sample pool for testing.
[0045] (2) Using optical tweezers Raman spectroscopy to test the red blood cell Raman spectrum: using the optical potential well generated by highly converged laser optical tweezers to move a single red blood cell in the sample pool to a relatively independent area, and adjust the objective lens to suspend the red blood cell in the sample At the height of 20-25 μm at the bottom of the pool, perform Raman spectrum detecti...
PUM
Property | Measurement | Unit |
---|---|---|
radius | aaaaa | aaaaa |
thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com